|Product Name||Anti-CD82 Picoband™ Antibody|
|Storage & Handling||At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing.|
|Description||Rabbit IgG polyclonal antibody for CD82 antigen(CD82) detection. Tested with WB, IHC-P in Human.|
|Cite This Product||Anti-CD82 Picoband™ Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9170)|
|Contents/Buffer||Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.|
|Immunogen||E.coli-derived human CD82 recombinant protein (Position: A98-Y267). Human CD82 shares 69% and 67% amino acid (aa) sequences identity with mouse and rat CD82, respectively.|
Assay Dilutions Overview
Immunohistochemistry(Paraffin-embedded Section), 0.5-1μg/ml, Human, By Heat
Western blot, 0.1-0.5μg/ml, Human
Boster's Secondary Antibodies And IHC, WB Kits
The following reagents are used to generate the images below.Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P).
Images And Assay Conditions
Anti-CD82 Picoband antibody, PB9170, Western blotting
All lanes: Anti CD82 (PB9170) at 0.5ug/ml
WB: Recombinant Human CD82 Protein 0.5ng
Predicted bind size: 36KD
Observed bind size: 36KD
Anti- CD82 Picoband antibody, PB9170, Western blotting
All lanes: Anti CD82 (PB9170) at 0.5ug/ml
Lane 1: HL-60 Whole Cell Lysate at 40ug
Lane 2: CEM Whole Cell Lysate at 40ug
Lane 3: HUT Whole Cell Lysate at 40ug
Lane 4: U937 Whole Cell Lysate at 40ug
Lane 5: MCF-7 Whole Cell Lysate at 40ug
Predicted bind size: 30KD
Observed bind size: 30KD
Anti-CD82 Picoband antibody, PB9170, IHC(P)
IHC(P): Human Tonsil Tissue
Protein Target Info (Source: Uniprot.org)
|Protein Name||CD82 antigen|
|Tissue Specificity||Lymphoid specific.|
|Alternative Names||CD82 antigen;C33 antigen;IA4;Inducible membrane protein R2;Metastasis suppressor Kangai-1;Suppressor of tumorigenicity 6 protein;Tetraspanin-27;Tspan-27;CD82;CD82;KAI1, SAR2, ST6, TSPAN27;|
|Subcellular Localization||Membrane; Multi-pass membrane protein.|
|Molecular Weight||29626 MW|
*if product is indicated to react with multiple species, protein info is based on the human gene.
|Protein Function||Associates with CD4 or CD8 and delivers costimulatory signals for the TCR/CD3 pathway.|
|Research Areas||Adaptive Immunity, Cancer, Cancer Susceptibility, Cell Type Markers, Epigenetics And Nuclear Signaling, Immunology, Nk Cells, Oncoproteins/Suppressors, Platelets, T Cells, Transcription, Tumor Suppressors
*You can search these to find other products in these research areas.
|Background||CD82(Cluster of Differentiation 82), also named KAI1, is a protein that in humans encoded by the CD82 gene. The gene is mapped to 11p11.2. This metastasis suppressor gene product is a membrane glycoprotein that is a member of the transmembrane 4 superfamily. Expression of this gene has been shown to be downregulated in tumor progression of human cancers and can be activated by p53 through a consensus binding sequence in the promoter. The expression of CD82 protein appears to be correlated with lymph node metastasis in esophageal squamous cell carcinoma(ESCC). And the CD82 overexpression can suppress tumor invasiveness and metastatic potential by inducing MMP9 inactivation via upregulation of TIMP1.|
Other Recommended Resources
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1. Post-translational modification:phosphorylation, methylation, glycosylation etc. These modifications prevent SDS molecules from binding to the target protein and thus make the band size appear larger than expected
2. Post-translational cleavage: this can cause smaller bands and or multiple bands
3. Alternative splicing: the same gene can have alternative splicing patterns generating different size proteins, all with reactivities to the antibody.
4. Amino Acid R chain charge: SDS binds to positive charges. The different size and charge of the Amino Acid side chains can affect the amount of SDS binding and thus affect the observed band size.
5. Multimers: Multimers are usually broken up in reducing conditions. However if the interactions between the multimers are strong, the band may appear higher.,