Data & Images
|Product Name||Anti-CD82 Picoband™ Antibody|
|Description||Rabbit IgG polyclonal antibody for CD82 antigen(CD82) detection. Tested with WB, IHC-P in Human.|
|Cite This Product||Anti-CD82 Picoband™ Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9170)|
|Replacement Item||This antibody may replace the following items: sc-1087|sc-17752|sc-5540|sc-5540-X from Santa Cruz Biotechnology.|
*Our Boster Guarantee covers the use of this product in the above tested applications.
**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.
|Recommended Detection Systems||Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P).
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2017!
|Immunogen||E.coli-derived human CD82 recombinant protein (Position: A98-Y267). Human CD82 shares 69% and 67% amino acid (aa) sequences identity with mouse and rat CD82, respectively.|
|Cross Reactivity||No cross reactivity with other proteins|
|Contents||Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
*carrier free antibody available upon request.
|Concentration||Add 0.2ml of distilled water will yield a concentration of 500ug/ml.|
|Storage||At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing.|
|Purification||Immunogen affinity purified.|
Protein Target Info (Source: Uniprot.org)
You can check the tissue specificity below for information on selecting positive and negative control.
|Protein Name||CD82 antigen|
|Molecular Weight||29626 MW|
|Protein Function||Associates with CD4 or CD8 and delivers costimulatory signals for the TCR/CD3 pathway.|
|Tissue Specificity||Lymphoid specific.|
|Sequence Similarities||Belongs to the tetraspanin (TM4SF) family.|
|Subcellular Localization||Membrane; Multi-pass membrane protein.|
|Alternative Names||CD82 antigen;C33 antigen;IA4;Inducible membrane protein R2;Metastasis suppressor Kangai-1;Suppressor of tumorigenicity 6 protein;Tetraspanin-27;Tspan-27;CD82;CD82;KAI1, SAR2, ST6, TSPAN27;|
|Research Areas|||immunology|adaptive immunity|t cells| immunology|cell type markers|nk cells|platelets| epigenetics and nuclear signaling|transcription|cancer susceptibility|tumor suppressors| cancer|oncoproteins/suppressors||
Background for CD82 antigen
Dilution Ratios/Recommended Concentrations
At Boster we strive to provide the best Anti-CD82 Picoband™ Antibody by testing all applications on non-spiked tissues and cell lines to ensure that the affinity of the antibody is enough to react to the endogenouse level of the target protein. Read more about our QC panel here.
|Recommended dilution ratios are listed below:|
Immunohistochemistry(Paraffin-embedded Section), 0.5-1μg/ml, Human, By Heat|
Western blot, 0.1-0.5μg/ml, Human
**Boster provides high sensitivity secondary antibody kits for Western blotting and IHC. For more info see Related Products below.
Anti-CD82 Picoband™ Antibody Images
Click the images to enlarge.
All lanes: Anti CD82 (PB9170) at 0.5ug/ml
WB: Recombinant Human CD82 Protein 0.5ng
Predicted bind size: 36KD
Observed bind size: 36KD
All lanes: Anti CD82 (PB9170) at 0.5ug/ml
Lane 1: HL-60 Whole Cell Lysate at 40ug
Lane 2: CEM Whole Cell Lysate at 40ug
Lane 3: HUT Whole Cell Lysate at 40ug
Lane 4: U937 Whole Cell Lysate at 40ug
Lane 5: MCF-7 Whole Cell Lysate at 40ug
Predicted bind size: 30KD
Observed bind size: 30KD
IHC(P): Human Tonsil Tissue
1. Post-translational modification:phosphorylation, methylation, glycosylation etc. These modifications prevent SDS molecules from binding to the target protein and thus make the band size appear larger than expected
2. Post-translational cleavage: this can cause smaller bands and or multiple bands
3. Alternative splicing: the same gene can have alternative splicing patterns generating different size proteins, all with reactivities to the antibody.
4. Amino Acid R chain charge: SDS binds to positive charges. The different size and charge of the Amino Acid side chains can affect the amount of SDS binding and thus affect the observed band size.
5. Multimers: Multimers are usually broken up in reducing conditions. However if the interactions between the multimers are strong, the band may appear higher.,