Anti-CD86 Antibody Picoband™

B7-2/CD86 antibody

Boster Bio Anti-CD86 Antibody Picoband™ catalog # PB9251. Tested in IHC, WB applications. This antibody reacts with Human, Mouse. Cited in 1 publication(s).

Product Info Summary

SKU: PB9251
Size: 100 μg/vial
Reactive Species: Human, Mouse
Host: Rabbit
Application: IHC, WB

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Product Name

Anti-CD86 Antibody Picoband™

View all B7-2/CD86 Antibodies

SKU/Catalog Number

PB9251

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-CD86 Antibody Picoband™ catalog # PB9251. Tested in IHC, WB applications. This antibody reacts with Human, Mouse.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-CD86 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9251)

Host

Rabbit

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E.coli-derived human CD86 recombinant protein (Position: A24-F329). Human CD86 shares 50% amino acid (aa) sequence identity with mouse CD86.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins

Reactive Species

PB9251 is reactive to CD86 in Human, Mouse

Applications

PB9251 is guaranteed for IHC, WB Boster Guarantee

Observed Molecular Weight

60-80 kDa

Calculated molecular weight

37.682kDa

Background of B7-2/CD86

Cluster of Differentiation 86 (also known as CD86 and B7-2) is a protein expressed on antigen-presenting cells that provides costimulatory signals necessary for T cell activation and survival. The CD86 gene encodes a type I membrane protein that is a member of the immunoglobulin superfamily. Using fluorescence in situ hybridization mapping, the CD86, like CD80, was mapped to human 3q21. The antigen presentation coactivators B71 and B72, which are important in other immune-mediated thyroid diseases, are important for lymphocytic infiltration and the immune response against thyroid carcinoma.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml
Immunohistochemistry(Paraffin-embedded Section), 2-5μg/ml

Validation Images & Assay Conditions

Gene/Protein Information For CD86 (Source: Uniprot.org, NCBI)

Gene Name

CD86

Full Name

T-lymphocyte activation antigen CD86

Weight

37.682kDa

Alternative Names

Activation B7-2 antigen; B70; B7-2 antigen; B72; B7-2; B-lymphocyte activation antigen B7-2; BU63; CD28 antigen ligand 2; CD28LG2B7-2 antigen); CD86 antigen; CD86 molecule; CD86; CTLA-4 counter-receptor B7.2; FUN-1; LAB72; MGC34413; T-lymphocyte activation antigen CD86 CD86 B7-2, B7.2, B70, CD28LG2, LAB72 CD86 molecule T-lymphocyte activation antigen CD86|B-lymphocyte activation antigen B7-2|BU63|CD86 antigen (CD28 antigen ligand 2, B7-2 antigen)|CTLA-4 counter-receptor B7.2|FUN-1

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on CD86, check out the CD86 Infographic

CD86 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for CD86: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

Hello CJ!

PB9251 has been cited in 1 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Near infrared light-triggered on-demand Cur release from Gel-PDA@Cur composite hydrogel for antibacterial wound healing
Species: Rat

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5 Customer Q&As for Anti-CD86 Antibody Picoband™

Question

My boss were happy with the WB result of your anti-CD86 antibody. However we have been able to see positive staining in foreskin cell membrane using this antibody. Is that expected? Could you tell me where is CD86 supposed to be expressed?

Verified Customer

Verified customer

Asked: 2020-04-22

Answer

According to literature, foreskin does express CD86. Generally CD86 expresses in cell membrane. Regarding which tissues have CD86 expression, here are a few articles citing expression in various tissues:
Brain, Pubmed ID: 14702039, 15489334
Foreskin, Pubmed ID: 7541777

Boster Scientific Support

Answered: 2020-04-22

Question

We are currently using anti-CD86 antibody PB9251 for human tissue, and we are satisfied with the ICC results. The species of reactivity given in the datasheet says human. Is it likely that the antibody can work on zebrafish tissues as well?

Verified Customer

Verified customer

Asked: 2019-12-26

Answer

The anti-CD86 antibody (PB9251) has not been tested for cross reactivity specifically with zebrafish tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in zebrafish you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2019-12-26

Question

We have seen staining in human foreskin. Any tips? Is anti-CD86 antibody supposed to stain foreskin positively?

Verified Customer

Verified customer

Asked: 2019-07-12

Answer

Based on literature foreskin does express CD86. Based on Uniprot.org, CD86 is expressed in leukocyte, brain, foreskin, among other tissues. Regarding which tissues have CD86 expression, here are a few articles citing expression in various tissues:
Brain, Pubmed ID: 14702039, 15489334
Foreskin, Pubmed ID: 7541777

Boster Scientific Support

Answered: 2019-07-12

Question

We are interested in using your anti-CD86 antibody for negative regulation of t cell proliferation studies. Has this antibody been tested with western blotting on human raji whole cell lysates? We would like to see some validation images before ordering.

Verified Customer

Verified customer

Asked: 2019-06-04

Answer

I appreciate your inquiry. This PB9251 anti-CD86 antibody is tested on human raji whole cell lysates, u937 cells. It is guaranteed to work for Flow Cytometry, IHC-F, ICC, WB in human. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.

Boster Scientific Support

Answered: 2019-06-04

Question

We ordered your anti-CD86 antibody for IHC-F on brain in a previous project. I am using human, and We want to use the antibody for WB next. My question regards examining brain as well as leukocyte in our next experiment. Could give a recommendation on which antibody would work the best for WB?

H. Yang

Verified customer

Asked: 2018-12-14

Answer

I viewed the website and datasheets of our anti-CD86 antibody and it appears that PB9251 has been validated on human in both IHC-F and WB. Thus PB9251 should work for your application. Our Boster satisfaction guarantee will cover this product for WB in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for WB detection and you can check out our website bosterbio.com to find out more information about them.

Boster Scientific Support

Answered: 2018-12-14

Order DetailsPrice
PB9251

100μg

$370
PB9251-10ug

10μg sample (liquid)

$99
PB9251-Biotin

100 μg Biotin conjugated

$570
PB9251-Cy3

100 μg Cy3 conjugated

$570
PB9251-Dylight488

100 μg Dylight488 conjugated

$570
PB9251-Dylight550

100 μg Dylight550 conjugated

$570
PB9251-Dylight594

100 μg Dylight594 conjugated

$570
PB9251-FITC

100 μg FITC conjugated

$570
PB9251-HRP

100 μg HRP conjugated

$570
PB9251-APC

100 μg APC conjugated

$670
PB9251-PE

100 μg PE conjugated

$670
PB9251-carrier-free

Carrier Free

$370

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