Product Info Summary
SKU: | A00220-1 |
---|---|
Size: | 100 μg/vial |
Reactive Species: | Mouse, Rat |
Host: | Rabbit |
Application: | ELISA, WB |
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Product info
Product Name
Anti-CD86 Antibody Picoband™
SKU/Catalog Number
A00220-1
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-CD86 Antibody Picoband™ catalog # A00220-1. Tested in ELISA, WB applications. This antibody reacts with Mouse, Rat.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-CD86 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00220-1)
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E. coli-derived rat CD86 recombinant protein (Position: V29-T248).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A00220-1 is reactive to CD86 in Mouse, Rat
Applications
A00220-1 is guaranteed for ELISA, WB Boster Guarantee
Observed Molecular Weight
60-80 kDa
Calculated molecular weight
37.682kDa
Background of B7-2/CD86
Cluster of Differentiation 86 (also known as CD86 and B7-2) is a protein expressed on antigen-presenting cells that provides costimulatory signals necessary for T cell activation and survival. The CD86 gene encodes a type I membrane protein that is a member of the immunoglobulin superfamily. Using fluorescence in situ hybridization mapping, the CD86, like CD80, was mapped to human 3q21. The antigen presentation coactivators B71 and B72, which are important in other immune-mediated thyroid diseases, are important for lymphocytic infiltration and the immune response against thyroid carcinoma.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Reconsitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml
Direct ELISA, 0.1-0.5μg/ml
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of CD86 using anti-CD86 antibody (A00220-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: rat spleen tissue lysate,
Lane 2: rat liver tissue lysate,
Lane 3: rat PC-12 cell lysate,
Lane 4: mouse spleen tissue lysate,
Lane 5: mouse thymus tissue lysate,
Lane 6: mouse brain tissue lysate,
Lane 7: human COLO-320 cell lysate.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD86 antigen affinity purified polyclonal antibody (Catalog # A00220-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CD86 at approximately 60-80KD. The expected band size for CD86 is at 36KD.
Protein Target Info & Infographic
Gene/Protein Information For CD86 (Source: Uniprot.org, NCBI)
Gene Name
CD86
Full Name
T-lymphocyte activation antigen CD86
Weight
37.682kDa
Alternative Names
Activation B7-2 antigen; B70; B7-2 antigen; B72; B7-2; B-lymphocyte activation antigen B7-2; BU63; CD28 antigen ligand 2; CD28LG2B7-2 antigen); CD86 antigen; CD86 molecule; CD86; CTLA-4 counter-receptor B7.2; FUN-1; LAB72; MGC34413; T-lymphocyte activation antigen CD86 CD86 B7-2, B7.2, B70, CD28LG2, LAB72 CD86 molecule T-lymphocyte activation antigen CD86|B-lymphocyte activation antigen B7-2|BU63|CD86 antigen (CD28 antigen ligand 2, B7-2 antigen)|CTLA-4 counter-receptor B7.2|FUN-1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CD86, check out the CD86 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CD86: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-CD86 Antibody Picoband™ (A00220-1)
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No publications found for A00220-1
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Customer Reviews
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Customer Q&As
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Find answers in Q&As, reviews.
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Submit your question
16 Customer Q&As for Anti-CD86 Antibody Picoband™
Question
Is a blocking peptide available for product anti-CD86 antibody (A00220-1)?
Verified Customer
Verified customer
Asked: 2020-04-29
Answer
We do provide the blocking peptide for product anti-CD86 antibody (A00220-1). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2020-04-29
Question
I see that the anti-CD86 antibody A00220-1 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2020-04-10
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2020-04-10
Question
I was wanting to use your anti-CD86 antibody for WB for mouse leukocyte on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for mouse leukocyte identification?
Verified Customer
Verified customer
Asked: 2020-04-01
Answer
As indicated on the product datasheet, A00220-1 anti-CD86 antibody has been validated for ELISA, WB on mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in mouse leukocyte in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2020-04-01
Question
We are currently using anti-CD86 antibody A00220-1 for mouse tissue, and we are satisfied with the ELISA results. The species of reactivity given in the datasheet says mouse, rat. Is it likely that the antibody can work on horse tissues as well?
Verified Customer
Verified customer
Asked: 2019-12-16
Answer
The anti-CD86 antibody (A00220-1) has not been tested for cross reactivity specifically with horse tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in horse you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-12-16
Question
Please see the WB image, lot number and protocol we used for leukocyte using anti-CD86 antibody A00220-1. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-11-14
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-11-14
Question
Would anti-CD86 antibody A00220-1 work for WB with leukocyte?
Verified Customer
Verified customer
Asked: 2019-09-20
Answer
According to the expression profile of leukocyte, CD86 is highly expressed in leukocyte. So, it is likely that anti-CD86 antibody A00220-1 will work for WB with leukocyte.
Boster Scientific Support
Answered: 2019-09-20
Question
We have seen staining in mouse brain. What should we do? Is anti-CD86 antibody supposed to stain brain positively?
Verified Customer
Verified customer
Asked: 2019-08-06
Answer
From what I have seen in literature brain does express CD86. From what I have seen in Uniprot.org, CD86 is expressed in leukocyte, brain, foreskin, among other tissues. Regarding which tissues have CD86 expression, here are a few articles citing expression in various tissues:
Brain, Pubmed ID: 14702039, 15489334
Foreskin, Pubmed ID: 7541777
Boster Scientific Support
Answered: 2019-08-06
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for leukocyte using anti-CD86 antibody A00220-1. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-08-05
Answer
I appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-08-05
Question
We ordered your anti-CD86 antibody for ELISA on foreskin a few years ago. I am using mouse, and We are going to use the antibody for WB next. I am interested in examining foreskin as well as leukocyte in our next experiment. Could you please give me some suggestion on which antibody would work the best for WB?
Verified Customer
Verified customer
Asked: 2019-03-13
Answer
I viewed the website and datasheets of our anti-CD86 antibody and it appears that A00220-1 has been validated on mouse in both ELISA and WB. Thus A00220-1 should work for your application. Our Boster satisfaction guarantee will cover this product for WB in mouse even if the specific tissue type has not been validated. We do have a comprehensive range of products for WB detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2019-03-13
Question
We need using your anti-CD86 antibody for cellular response to metal ion studies. Has this antibody been tested with western blotting on tissue lysate? We would like to see some validation images before ordering.
A. Edwards
Verified customer
Asked: 2019-02-15
Answer
We appreciate your inquiry. This A00220-1 anti-CD86 antibody is tested on rat liver tissue, spleen tissue, tissue lysate, mouse thymus tissue, brain tissue. It is guaranteed to work for ELISA, WB in mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2019-02-15
Question
Is this A00220-1 anti-CD86 antibody reactive to the isotypes of CD86?
P. Krishna
Verified customer
Asked: 2018-12-05
Answer
The immunogen of A00220-1 anti-CD86 antibody is E. coli-derived rat CD86 recombinant protein (Position: V29-T248). Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2018-12-05
Question
Does A00220-1 anti-CD86 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2018-10-15
Answer
You can see on the product datasheet, A00220-1 anti-CD86 antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2018-10-15
Question
My question regarding product A00220-1, anti-CD86 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2018-08-09
Answer
We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A00220-1 anti-CD86 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2018-08-09
Question
My question regards to test anti-CD86 antibody A00220-1 on mouse leukocyte for research purposes, then I may be interested in using anti-CD86 antibody A00220-1 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2018-05-22
Answer
The products we sell, including anti-CD86 antibody A00220-1, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2018-05-22
Question
Do you have a BSA free version of anti-CD86 antibody A00220-1 available?
A. Wu
Verified customer
Asked: 2017-02-22
Answer
I appreciate your recent telephone inquiry. I can confirm that some lots of this anti-CD86 antibody A00220-1 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2017-02-22
Question
My team were content with the WB result of your anti-CD86 antibody. However we have been able to see positive staining in leukocyte cell membrane using this antibody. Is that expected? Could you tell me where is CD86 supposed to be expressed?
K. Jones
Verified customer
Asked: 2015-09-23
Answer
From what I have seen in literature, leukocyte does express CD86. Generally CD86 expresses in cell membrane. Regarding which tissues have CD86 expression, here are a few articles citing expression in various tissues:
Brain, Pubmed ID: 14702039, 15489334
Foreskin, Pubmed ID: 7541777
Boster Scientific Support
Answered: 2015-09-23