Anti-CD86 Antibody Picoband™

B7-2/CD86 antibody

Boster Bio Anti-CD86 Antibody Picoband™ catalog # A00220-1. Tested in ELISA, WB applications. This antibody reacts with Mouse, Rat.

Product Info Summary

SKU: A00220-1
Size: 100 μg/vial
Reactive Species: Mouse, Rat
Host: Rabbit
Application: ELISA, WB

Product Name

Anti-CD86 Antibody Picoband™

View all B7-2/CD86 Antibodies

SKU/Catalog Number

A00220-1

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-CD86 Antibody Picoband™ catalog # A00220-1. Tested in ELISA, WB applications. This antibody reacts with Mouse, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-CD86 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00220-1)

Host

Rabbit

Contents

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E. coli-derived rat CD86 recombinant protein (Position: V29-T248).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

A00220-1 is reactive to CD86 in Mouse, Rat

Applications

A00220-1 is guaranteed for ELISA, WB Boster Guarantee

Observed Molecular Weight

60-80 kDa

Calculated molecular weight

37.682kDa

Background of B7-2/CD86

Cluster of Differentiation 86 (also known as CD86 and B7-2) is a protein expressed on antigen-presenting cells that provides costimulatory signals necessary for T cell activation and survival. The CD86 gene encodes a type I membrane protein that is a member of the immunoglobulin superfamily. Using fluorescence in situ hybridization mapping, the CD86, like CD80, was mapped to human 3q21. The antigen presentation coactivators B71 and B72, which are important in other immune-mediated thyroid diseases, are important for lymphocytic infiltration and the immune response against thyroid carcinoma.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Innovating Scientists Reward

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml
Direct ELISA, 0.1-0.5μg/ml

Validation Images & Assay Conditions

Gene/Protein Information For CD86 (Source: Uniprot.org, NCBI)

Gene Name

CD86

Full Name

T-lymphocyte activation antigen CD86

Weight

37.682kDa

Alternative Names

Activation B7-2 antigen; B70; B7-2 antigen; B72; B7-2; B-lymphocyte activation antigen B7-2; BU63; CD28 antigen ligand 2; CD28LG2B7-2 antigen); CD86 antigen; CD86 molecule; CD86; CTLA-4 counter-receptor B7.2; FUN-1; LAB72; MGC34413; T-lymphocyte activation antigen CD86 CD86 B7-2, B7.2, B70, CD28LG2, LAB72 CD86 molecule T-lymphocyte activation antigen CD86|B-lymphocyte activation antigen B7-2|BU63|CD86 antigen (CD28 antigen ligand 2, B7-2 antigen)|CTLA-4 counter-receptor B7.2|FUN-1

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on CD86, check out the CD86 Infographic

CD86 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for CD86: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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16 Customer Q&As for Anti-CD86 Antibody Picoband™

Question

Is a blocking peptide available for product anti-CD86 antibody (A00220-1)?

Verified Customer

Verified customer

Asked: 2020-04-29

Answer

We do provide the blocking peptide for product anti-CD86 antibody (A00220-1). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2020-04-29

Question

I see that the anti-CD86 antibody A00220-1 works with WB, what is the protocol used to produce the result images on the product page?

Verified Customer

Verified customer

Asked: 2020-04-10

Answer

You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2020-04-10

Question

I was wanting to use your anti-CD86 antibody for WB for mouse leukocyte on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for mouse leukocyte identification?

Verified Customer

Verified customer

Asked: 2020-04-01

Answer

As indicated on the product datasheet, A00220-1 anti-CD86 antibody has been validated for ELISA, WB on mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in mouse leukocyte in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2020-04-01

Question

We are currently using anti-CD86 antibody A00220-1 for mouse tissue, and we are satisfied with the ELISA results. The species of reactivity given in the datasheet says mouse, rat. Is it likely that the antibody can work on horse tissues as well?

Verified Customer

Verified customer

Asked: 2019-12-16

Answer

The anti-CD86 antibody (A00220-1) has not been tested for cross reactivity specifically with horse tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in horse you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2019-12-16

Question

Please see the WB image, lot number and protocol we used for leukocyte using anti-CD86 antibody A00220-1. Please let me know if you require anything else.

Verified Customer

Verified customer

Asked: 2019-11-14

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2019-11-14

Question

Would anti-CD86 antibody A00220-1 work for WB with leukocyte?

Verified Customer

Verified customer

Asked: 2019-09-20

Answer

According to the expression profile of leukocyte, CD86 is highly expressed in leukocyte. So, it is likely that anti-CD86 antibody A00220-1 will work for WB with leukocyte.

Boster Scientific Support

Answered: 2019-09-20

Question

We have seen staining in mouse brain. What should we do? Is anti-CD86 antibody supposed to stain brain positively?

Verified Customer

Verified customer

Asked: 2019-08-06

Answer

From what I have seen in literature brain does express CD86. From what I have seen in Uniprot.org, CD86 is expressed in leukocyte, brain, foreskin, among other tissues. Regarding which tissues have CD86 expression, here are a few articles citing expression in various tissues:
Brain, Pubmed ID: 14702039, 15489334
Foreskin, Pubmed ID: 7541777

Boster Scientific Support

Answered: 2019-08-06

Question

We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for leukocyte using anti-CD86 antibody A00220-1. Let me know if you need anything else.

Verified Customer

Verified customer

Asked: 2019-08-05

Answer

I appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2019-08-05

Question

We ordered your anti-CD86 antibody for ELISA on foreskin a few years ago. I am using mouse, and We are going to use the antibody for WB next. I am interested in examining foreskin as well as leukocyte in our next experiment. Could you please give me some suggestion on which antibody would work the best for WB?

Verified Customer

Verified customer

Asked: 2019-03-13

Answer

I viewed the website and datasheets of our anti-CD86 antibody and it appears that A00220-1 has been validated on mouse in both ELISA and WB. Thus A00220-1 should work for your application. Our Boster satisfaction guarantee will cover this product for WB in mouse even if the specific tissue type has not been validated. We do have a comprehensive range of products for WB detection and you can check out our website bosterbio.com to find out more information about them.

Boster Scientific Support

Answered: 2019-03-13

Question

We need using your anti-CD86 antibody for cellular response to metal ion studies. Has this antibody been tested with western blotting on tissue lysate? We would like to see some validation images before ordering.

A. Edwards

Verified customer

Asked: 2019-02-15

Answer

We appreciate your inquiry. This A00220-1 anti-CD86 antibody is tested on rat liver tissue, spleen tissue, tissue lysate, mouse thymus tissue, brain tissue. It is guaranteed to work for ELISA, WB in mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.

Boster Scientific Support

Answered: 2019-02-15

Question

Is this A00220-1 anti-CD86 antibody reactive to the isotypes of CD86?

P. Krishna

Verified customer

Asked: 2018-12-05

Answer

The immunogen of A00220-1 anti-CD86 antibody is E. coli-derived rat CD86 recombinant protein (Position: V29-T248). Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2018-12-05

Question

Does A00220-1 anti-CD86 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

Verified Customer

Verified customer

Asked: 2018-10-15

Answer

You can see on the product datasheet, A00220-1 anti-CD86 antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2018-10-15

Question

My question regarding product A00220-1, anti-CD86 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

Verified Customer

Verified customer

Asked: 2018-08-09

Answer

We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A00220-1 anti-CD86 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2018-08-09

Question

My question regards to test anti-CD86 antibody A00220-1 on mouse leukocyte for research purposes, then I may be interested in using anti-CD86 antibody A00220-1 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

Verified Customer

Verified customer

Asked: 2018-05-22

Answer

The products we sell, including anti-CD86 antibody A00220-1, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2018-05-22

Question

Do you have a BSA free version of anti-CD86 antibody A00220-1 available?

A. Wu

Verified customer

Asked: 2017-02-22

Answer

I appreciate your recent telephone inquiry. I can confirm that some lots of this anti-CD86 antibody A00220-1 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2017-02-22

Question

My team were content with the WB result of your anti-CD86 antibody. However we have been able to see positive staining in leukocyte cell membrane using this antibody. Is that expected? Could you tell me where is CD86 supposed to be expressed?

K. Jones

Verified customer

Asked: 2015-09-23

Answer

From what I have seen in literature, leukocyte does express CD86. Generally CD86 expresses in cell membrane. Regarding which tissues have CD86 expression, here are a few articles citing expression in various tissues:
Brain, Pubmed ID: 14702039, 15489334
Foreskin, Pubmed ID: 7541777

Boster Scientific Support

Answered: 2015-09-23

Order DetailsPrice
A00220-1

100μg

$370
A00220-1-10ug

10μg sample (liquid)

$99
A00220-1-Biotin

100 μg Biotin conjugated

$570
A00220-1-Cy3

100 μg Cy3 conjugated

$570
A00220-1-Dylight488

100 μg Dylight488 conjugated

$570
A00220-1-Dylight550

100 μg Dylight550 conjugated

$570
A00220-1-Dylight594

100 μg Dylight594 conjugated

$570
A00220-1-FITC

100 μg FITC conjugated

$570
A00220-1-HRP

100 μg HRP conjugated

$570
A00220-1-APC

100 μg APC conjugated

$670
A00220-1-PE

100 μg PE conjugated

$670
A00220-1-carrier-free

Carrier Free

$370

More conjugates/formats

Free Secondary Antibody

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A00220-1
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00

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