Product Info Summary
SKU: | A04573 |
---|---|
Size: | 100μl |
Reactive Species: | Human, Mouse |
Host: | Rabbit |
Application: | WB |
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Product info
Product Name
Anti-Cdc16/Apc6 Antibody
SKU/Catalog Number
A04573
Size
100μl
Form
Liquid
Description
Boster Bio Anti-Cdc16/Apc6 Antibody catalog # A04573. Tested in WB applications. This antibody reacts with Human, Mouse.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Cdc16/Apc6 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A04573)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Clonality
Polyclonal
Clone Number
5G9
Isotype
IgG
Immunogen
Synthesized peptide derived from human Cdc16 around the non-phosphorylation site of S560.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A04573 is reactive to CDC16 in Human, Mouse
Applications
A04573 is guaranteed for WB Boster Guarantee
Observed Molecular Weight
39 kDa
Calculated molecular weight
71.656kDa
Background of CDC16
May play an important role in modulating bone turnover. Promotes the adhesion of osteoblast cells and inhibits the binding of fibrinogen to integrin receptors. In addition, inhibits osteocalcin production.
Pennica D., Swanson T.A., Welsh J.W. J.Proc. Natl. Acad. Sci. U.S.A. 95:14717-14722(1998)
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Innovating Scientists Reward
If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB, 1:500-1:2000
Validation Images & Assay Conditions
Click image to see more details
Western Blot (WB) analysis of specific cells using Cdc16 Polyclonal antibody.
Protein Target Info & Infographic
Gene/Protein Information For CDC16 (Source: Uniprot.org, NCBI)
Gene Name
CDC16
Full Name
Cell division cycle protein 16 homolog
Weight
71.656kDa
Superfamily
APC6/CDC16 family
Alternative Names
ANAPC6subunit 6; CDC16 (cell division cycle 16, S. cerevisiae, homolog); CDC16 cell division cycle 16 homolog (S. cerevisiae); CDC16 homolog; CDC16Hs; cell division cycle 16 homolog (S. cerevisiae); cell division cycle protein 16 homolog; Cyclosome subunit 6 CDC16 ANAPC6, APC6Hs, CUT9, CDC16 cell division cycle 16 cell division cycle protein 16 homolog|anaphase-promoting complex, subunit 6|cell division cycle 16 homolog|cyclosome subunit 6
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CDC16, check out the CDC16 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CDC16: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Cdc16/Apc6 Antibody (A04573)
Hello CJ!
No publications found for A04573
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Customer Q&As
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12 Customer Q&As for Anti-Cdc16/Apc6 Antibody
Question
My lab would like to test anti-Cdc16/Apc6 antibody A04573 on mouse right uterine tube for research purposes, then I may be interested in using anti-Cdc16/Apc6 antibody A04573 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2020-03-04
Answer
The products we sell, including anti-Cdc16/Apc6 antibody A04573, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2020-03-04
Question
I was wanting to use your anti-Cdc16/Apc6 antibody for WB for mouse right uterine tube on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for mouse right uterine tube identification?
Verified Customer
Verified customer
Asked: 2019-12-16
Answer
As indicated on the product datasheet, A04573 anti-Cdc16/Apc6 antibody has been tested for WB on human, mouse tissues. We have an innovator award program that if you test this antibody and show it works in mouse right uterine tube in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-12-16
Question
Can you help my question with product A04573, anti-Cdc16/Apc6 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-12-04
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A04573 anti-Cdc16/Apc6 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-12-04
Question
Is a blocking peptide available for product anti-Cdc16/Apc6 antibody (A04573)?
Verified Customer
Verified customer
Asked: 2019-08-08
Answer
We do provide the blocking peptide for product anti-Cdc16/Apc6 antibody (A04573). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2019-08-08
Question
Thanks for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for right uterine tube using anti-Cdc16/Apc6 antibody A04573. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-06-10
Answer
Thanks for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-06-10
Question
Would anti-Cdc16/Apc6 antibody A04573 work for WB with right uterine tube?
J. Krishna
Verified customer
Asked: 2018-07-27
Answer
According to the expression profile of right uterine tube, CDC16 is highly expressed in right uterine tube. So, it is likely that anti-Cdc16/Apc6 antibody A04573 will work for WB with right uterine tube.
Boster Scientific Support
Answered: 2018-07-27
Question
Is there a BSA free version of anti-Cdc16/Apc6 antibody A04573 available?
Verified Customer
Verified customer
Asked: 2017-12-12
Answer
I appreciate your recent telephone inquiry. I can confirm that some lots of this anti-Cdc16/Apc6 antibody A04573 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2017-12-12
Question
Does A04573 anti-Cdc16/Apc6 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2017-07-04
Answer
You can see on the product datasheet, A04573 anti-Cdc16/Apc6 antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2017-07-04
Question
Does anti-Cdc16/Apc6 antibody A04573 work on goat WB with right uterine tube?
K. Jones
Verified customer
Asked: 2015-09-23
Answer
Our lab technicians have not tested anti-Cdc16/Apc6 antibody A04573 on goat. You can run a BLAST between goat and the immunogen sequence of anti-Cdc16/Apc6 antibody A04573 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated goat samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in goat right uterine tube in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2015-09-23
Question
Is this A04573 anti-Cdc16/Apc6 antibody reactive to the isotypes of CDC16?
S. Jha
Verified customer
Asked: 2015-06-16
Answer
The immunogen of A04573 anti-Cdc16/Apc6 antibody is Synthesized peptide derived from human Cdc16 around the non-phosphorylation site of S560. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2015-06-16
Question
I see that the anti-Cdc16/Apc6 antibody A04573 works with WB, what is the protocol used to produce the result images on the product page?
A. Walker
Verified customer
Asked: 2015-04-24
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2015-04-24
Question
See below the WB image, lot number and protocol we used for right uterine tube using anti-Cdc16/Apc6 antibody A04573. Please let me know if you require anything else.
N. Brown
Verified customer
Asked: 2013-02-19
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2013-02-19