Data & Images
|Product Name||Anti-Cdc25B Antibody|
|Description||Rabbit IgG polyclonal antibody for M-phase inducer phosphatase 2(CDC25B) detection. Tested with WB, IHC-P in Human;Rat.|
|Cite This Product||Anti-Cdc25B Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1546)|
|Replacement Item||This antibody may replace the following items: sc-56357|sc-6949|sc-5619|sc-6948|sc-65504|sc-56266|sc-326|sc-70825 from Santa Cruz Biotechnology.|
|Validated Species||Human, Rat|
|Predicted Species||Canine, Horse, Monkey, Rabbit|
*This antibody is predicted to react with the above species based on antigen sequence similarities. Our Boster Guarantee covers the use of this product with the above species.
*Our Boster Guarantee covers the use of this product in the above tested applications.
**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.
|Recommended Detection Systems||Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P).
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2017!
|Immunogen||A synthetic peptide corresponding to a sequence at the C-terminus of human Cdc25B(541-559aa DYRPMNHEAFKDELKTFRL), different from the related mouse sequence by three amino acids.|
|Cross Reactivity||No cross reactivity with other proteins|
|Contents||Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
*carrier free antibody available upon request.
|Concentration||Add 0.2ml of distilled water will yield a concentration of 500ug/ml.|
|Storage||At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing.|
|Purification||Immunogen affinity purified.|
Protein Target Info (Source: Uniprot.org)
You can check the tissue specificity below for information on selecting positive and negative control.
|Protein Name||M-phase inducer phosphatase 2|
|Molecular Weight||64987 MW|
|Protein Function||Tyrosine protein phosphatase which functions as a dosage-dependent inducer of mitotic progression. Required for G2/M phases of the cell cycle progression and abscission during cytokinesis in a ECT2-dependent manner. Directly dephosphorylates CDK1 and stimulates its kinase activity. The three isoforms seem to have a different level of activity. .|
|Sequence Similarities||Belongs to the MPI phosphatase family.|
|Subcellular Localization||Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Cytoplasm, cytoskeleton, spindle pole.|
|Alternative Names||M-phase inducer phosphatase 2;22.214.171.124;Dual specificity phosphatase Cdc25B;CDC25B;CDC25HU2;|
|Research Areas|||cell biology|cell cycle|kinases/phosphatases| epigenetics and nuclear signaling|phospatases| cancer||
Background for M-phase inducer phosphatase 2
Dilution Ratios/Recommended Concentrations
At Boster we strive to provide the best Anti-Cdc25B Antibody by testing all applications on non-spiked tissues and cell lines to ensure that the affinity of the antibody is enough to react to the endogenouse level of the target protein. Read more about our QC panel here.
|Recommended dilution ratios are listed below:|
Immunohistochemistry(Paraffin-embedded Section), 0.5-1μg/ml, Human, Rat, By Heat|
Western blot, 0.1-0.5μg/ml, Rat, Human
**Boster provides high sensitivity secondary antibody kits for Western blotting and IHC. For more info see Related Products below.
Anti-Cdc25B Antibody Images
Click the images to enlarge.
Lane 1: Rat Brain Tissue Lysate
Lane 2: Rat Kidney Tissue Lysate
Lane 3: Rat Lung Tissue Lysate
IHC(P): Human Lung Cancer Tissue
1. Post-translational modification:phosphorylation, methylation, glycosylation etc. These modifications prevent SDS molecules from binding to the target protein and thus make the band size appear larger than expected
2. Post-translational cleavage: this can cause smaller bands and or multiple bands
3. Alternative splicing: the same gene can have alternative splicing patterns generating different size proteins, all with reactivities to the antibody.
4. Amino Acid R chain charge: SDS binds to positive charges. The different size and charge of the Amino Acid side chains can affect the amount of SDS binding and thus affect the observed band size.
5. Multimers: Multimers are usually broken up in reducing conditions. However if the interactions between the multimers are strong, the band may appear higher.,