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SKU:MA1018
Pack Size:100μg/vial
Sample Size:30ug for $99, contact us for details
Clonality:Monoclonal
Clone Number:DCS-193
Application:WB
Price: $200.00
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Overview

Product Name Anti-CDC25C Antibody (Monoclonal, DCS-193)
SKU/Catalog Number MA1018
Description Mouse IgG monoclonal antibody for CDC25C, cell division cycle 25 homolog C (S. pombe) (CDC25C) detection. Tested with WB in Human. No cross reactivity with other proteins.
Cite This Product Anti-CDC25C Antibody (Monoclonal, DCS-193) DCS-193 (Boster Biological Technology, Pleasanton CA, USA, Catalog # MA1018)
Replacement Item This antibody may replace the following items: sc-56269|sc-6950|sc-13138|sc-5620|sc-55513|sc-327 from Santa Cruz Biotechnology.
Host Mouse
Isotype Mouse IgG1
Validated Species Human
Application WB

*Our Boster Guarantee covers the use of this product in the above tested applications.

**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.

Recommended Detection Systems Boster recommends Enhanced Chemiluminescent Kit with anti-Mouse IgG (EK1001) for Western blot.
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2017!
Immunogen Recombinant human Cdc25c.
Cross Reactivity No cross reactivity with other proteins
Pack Size 100μg/vial

Properties

Clonality Monoclonal
Clone Number DCS-193
Form Lyophilized
Contents Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.
*carrier free antibody available upon request.
Concentration Add 1ml of PBS buffer will yield a concentration of 100ug/ml.
Storage At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing.
Purification Ascites
Isotype Mouse IgG1

Protein Target Info (Source: Uniprot.org)

You can check the tissue specificity below for information on selecting positive and negative control.

Gene Name Cdc25c
Protein Name M-phase inducer phosphatase 3
Molecular Weight 50896 MW
Protein Function CDC25C: Functions as a dosage-dependent inducer in mitoticcontrol. Tyrosine protein phosphatase required for progression ofthe cell cycle. When phosphorylated, highly effective inactivating G2 cells into prophase. Directly dephosphorylates CDK1and activates its kinase activity.
Sequence Similarities Belongs to the MPI phosphatase family.
Subcellular Localization CDC25C: Nucleus
Cdc25c: Nucleus.
Uniprot ID D4ADT2
Alternative Names Protein Cdc25c ;Cdc25c ;
Research Areas |cell biology|cell cycle|kinases/phosphatases| epigenetics and nuclear signaling|phospatases|
*if product is indicated to react with multiple species, protein info is based on the human gene.

Background for M-phase inducer phosphatase 3

Cell division sycle(CDC25) gene product is a protein-tyrosine phosphatase, activates a partially purified p34(cdc2)/cyclin B complex. The cdc25 protein also shares homology with a protein phosphatase with activity against both tyrosine and serine(and thus probably threonine) phosphate residues. CDC25C is expressed predominantly in G2 phase in healer cells. The human gene encodes a protein with a predicted molecular mass of 53,000 daltons whose C-terminal domain shares about 37% sequence identity with the fission yeast cdc25 mitotic inducer. CDC25C gene is mapped to 5q31.
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Publications

Ling H, Lu Lf, He J, Xiao Gh, Jiang H, Su Q. Oncol Rep. 2014 Nov;32(5):2274-82. Doi: 10.3892/Or.2014.3417. Epub 2014 Aug 19. Diallyl Disulfide Selectively Causes Checkpoint Kinase-1 Mediated G2/M Arrest In Human Mgc803 Gastric Cancer Cell Line.

FAQs

Q: Is your western blot protocol provided from the website applicable for all your antibodies? Keyword: applications, WB
A: The protocol is applicable for all our antibodies in WB, the NC Membrane(0.45μm or 0.22μm) and transfer time(70 mins or 50 mins) depends on the protein molecular weight, details can be found in included protocol.
Q: Can I conjugate markers to this antibody? Can I link custom conjugates to this antibody? Keyword: conjugation
A: The antibody is stored with BSA and cannot be conjugated with markers. Carrier free antibodies are available upon request. Please contact support@bosterbio.com
Q: Can you provide monoclonal antibodies carrier free?
A: We cannot.
Q: What are some alternative names that could be used to describe this product?
A: Some common names include but are not limited to cdc25 antibody, cdc25c antibody