Data & Images
|Product Name||Anti-CDC25C Antibody (Monoclonal, DCS-193)|
|Description||Mouse IgG monoclonal antibody for CDC25C, cell division cycle 25 homolog C (S. pombe) (CDC25C) detection. Tested with WB in Human. No cross reactivity with other proteins.|
|Cite This Product||Anti-CDC25C Antibody (Monoclonal, DCS-193) DCS-193 (Boster Biological Technology, Pleasanton CA, USA, Catalog # MA1018)|
|Replacement Item||This antibody may replace the following items: sc-56269|sc-6950|sc-13138|sc-5620|sc-55513|sc-327 from Santa Cruz Biotechnology.|
*Our Boster Guarantee covers the use of this product in the above tested applications.
**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.
|Recommended Detection Systems||Boster recommends Enhanced Chemiluminescent Kit with anti-Mouse IgG (EK1001) for Western blot.
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2017!
|Immunogen||Recombinant human Cdc25c.|
|Cross Reactivity||No cross reactivity with other proteins|
|Contents||Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.
*carrier free antibody available upon request.
|Concentration||Add 1ml of PBS buffer will yield a concentration of 100ug/ml.|
|Storage||At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing.|
Protein Target Info (Source: Uniprot.org)
You can check the tissue specificity below for information on selecting positive and negative control.
|Protein Name||M-phase inducer phosphatase 3|
|Molecular Weight||50896 MW|
|Protein Function||CDC25C: Functions as a dosage-dependent inducer in mitoticcontrol. Tyrosine protein phosphatase required for progression ofthe cell cycle. When phosphorylated, highly effective inactivating G2 cells into prophase. Directly dephosphorylates CDK1and activates its kinase activity.|
|Sequence Similarities||Belongs to the MPI phosphatase family.|
|Subcellular Localization||CDC25C: Nucleus
|Alternative Names||Protein Cdc25c ;Cdc25c ;|
|Research Areas|||cell biology|cell cycle|kinases/phosphatases| epigenetics and nuclear signaling|phospatases||
Background for M-phase inducer phosphatase 3
Dilution Ratios/Recommended Concentrations
At Boster we strive to provide the best Anti-CDC25C Antibody (Monoclonal, DCS-193) by testing all applications on non-spiked tissues and cell lines to ensure that the affinity of the antibody is enough to react to the endogenouse level of the target protein. Read more about our QC panel here.
|Recommended dilution ratios are listed below:|
Western blot, 1-2μg/ml, Human|
**Boster provides high sensitivity secondary antibody kits for Western blotting and IHC. For more info see Related Products below.