Anti-CDC45/Cdc45L Rabbit Monoclonal Antibody

CDC45L antibody

Boster Bio Anti-CDC45/Cdc45L Rabbit Monoclonal Antibody catalog # M01367. Tested in WB, IHC, ICC/IF, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.

Product Info Summary

SKU: M01367
Size: 100 μl
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: Flow Cytometry, IF, IHC, ICC, WB

Product Name

Anti-CDC45/Cdc45L Rabbit Monoclonal Antibody

View all CDC45L Antibodies

SKU/Catalog Number

M01367

Size

100 μl

Form

Liquid

Description

Boster Bio Anti-CDC45/Cdc45L Rabbit Monoclonal Antibody catalog # M01367. Tested in WB, IHC, ICC/IF, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-CDC45/Cdc45L Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01367)

Host

Rabbit

Contents

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.

Clonality

Monoclonal

Clone Number

AD-3

Isotype

Rabbit IgG

Immunogen

A synthesized peptide derived from human CDC45

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

Detects ~20kDa. Does not cross-react with αB-crystallin, βL-crystallin, ΒH- crystallin, γ-crystallin, HSP25, HSP27 or HSP47 proteins.

Reactive Species

M01367 is reactive to CDC45 in Human, Mouse, Rat

Applications

M01367 is guaranteed for Flow Cytometry, IF, IHC, ICC, WB Boster Guarantee

Observed Molecular Weight

40-60 kDa

Calculated molecular weight

65.569kDa

Background of CDC45L

Furin is likely to represent the ubiquitous endoprotease activity within constitutive secretory pathways and capable of cleavage at the RX (K/R) R consensus motif.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Innovating Scientists Reward

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Reconsitution

Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB 1:500-1:2000
IHC 1:50-1:200
ICC/IF 1:50-1:200
FC 1:50

Validation Images & Assay Conditions

Gene/Protein Information For CDC45 (Source: Uniprot.org, NCBI)

Gene Name

CDC45

Full Name

Cell division control protein 45 homolog

Weight

65.569kDa

Superfamily

CDC45 family

Alternative Names

CDC45 cell division cycle 45-like (S. cerevisiae); cell division cycle 45 homolog (S. cerevisiae); cell division cycle 45-like 2; human CDC45; S.cerevisiae, homolog)-like CDC45 CDC45LL2, MGORS7, PORC-PI-1, CDC45 cell division cycle 45 cell division control protein 45 homolog|CDC45 cell division cycle 45 homolog|CDC45 cell division cycle 45-like|CDC45-related protein|cell division cycle 45 homolog|cell division cycle 45-like 2|human CDC45

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on CDC45, check out the CDC45 Infographic

CDC45 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for CDC45: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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4 Customer Q&As for Anti-CDC45/Cdc45L Rabbit Monoclonal Antibody

Question

I see that the anti-CDC45/Cdc45L Rabbit Monoclonal antibody M01367 works with WB, what is the protocol used to produce the result images on the product page?

Verified Customer

Verified customer

Asked: 2019-06-18

Answer

You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2019-06-18

Question

I was wanting to use your anti-CDC45/Cdc45L Rabbit Monoclonal antibody for WB for rat cervix carcinoma on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for rat cervix carcinoma identification?

Verified Customer

Verified customer

Asked: 2018-07-25

Answer

It shows on the product datasheet, M01367 anti-CDC45/Cdc45L Rabbit Monoclonal antibody has been validated for IF, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in rat cervix carcinoma in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2018-07-25

Question

My lab would like to test anti-CDC45/Cdc45L Rabbit Monoclonal antibody M01367 on rat cervix carcinoma for research purposes, then I may be interested in using anti-CDC45/Cdc45L Rabbit Monoclonal antibody M01367 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

Verified Customer

Verified customer

Asked: 2017-09-11

Answer

The products we sell, including anti-CDC45/Cdc45L Rabbit Monoclonal antibody M01367, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2017-09-11

Question

My question regarding product M01367, anti-CDC45/Cdc45L Rabbit Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

A. Patel

Verified customer

Asked: 2016-12-08

Answer

We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M01367 anti-CDC45/Cdc45L Rabbit Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2016-12-08

Order DetailsPrice
M01367

100μL

$370
M01367-30ul

30μL sample

$99
M01367-carrier-free

Carrier Free

$370

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Free Secondary Antibody

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M01367
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00

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