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SKU:MA1019
Clonality:Monoclonal
Clone Number:DCS-180
Application:WB
Price: $200.00
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Overview

Product Name Anti-CDC6 Antibody (Monoclonal, DCS-180)
Description Mouse IgG monoclonal antibody for CDC6, cell division cycle 6 homolog (S. cerevisiae) (CDC6) detection. Tested with WB in Human. No cross reactivity with other proteins.
Cite This Product Anti-CDC6 Antibody (Monoclonal, DCS-180) DCS-180 (Boster Biological Technology, Pleasanton CA, USA, Catalog # MA1019)
Replacement Item This antibody may replace the following items: sc-70826|sc-70827|sc-28553|sc-8341|sc-56273|sc-13136|sc-32507|sc-9964|sc-6318|sc-6317 from Santa Cruz Biotechnology.
Host Mouse
Isotype Mouse IgG1
Validated Species Human
Application WB

*Our Boster Guarantee covers the use of this product in the above tested applications.

**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.

Recommended Detection Systems Boster recommends Enhanced Chemiluminescent Kit with anti-Mouse IgG (EK1001) for Western blot.
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2017!
Immunogen Recombinant human Cdc6.
Cross Reactivity No cross reactivity with other proteins
Pack Size 100μg/vial

Properties

Clonality Monoclonal
Clone Number DCS-180
Form Lyophilized
Contents Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.
*carrier free antibody available upon request.
Concentration Add 1ml of PBS buffer will yield a concentration of 100ug/ml.
Storage At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing.
Purification Ascites
Isotype Mouse IgG1

Protein Target Info (Source: Uniprot.org)

You can check the tissue specificity below for information on selecting positive and negative control.

Gene Name Cdc6
Protein Name Cell division control protein 6 homolog
Molecular Weight 62614 MW
Protein Function Involved in the initiation of DNA replication. Also participates in checkpoint controls that ensure DNA replication is completed before mitosis is initiated.
Sequence Similarities Belongs to the CDC6/cdc18 family.
Subcellular Localization Nucleus . Cytoplasm . The protein is nuclear in G1 and cytoplasmic in S-phase cells. .
Uniprot ID O89033
Alternative Names Cell division control protein 6 homolog;CDC6-related protein;p62(cdc6);Cdc6;
Research Areas |epigenetics and nuclear signaling|dna / rna|dna synthesis| cell biology|cell cycle|cell division|other cell division antibodies|
*if product is indicated to react with multiple species, protein info is based on the human gene.

Background for Cell division control protein 6 homolog

In yeasts, Cdc6(Saccharomyces cerevisiae) and Cdc18(Schizosaccharomyces pombe) associate with the origin recognition complex(ORC) proteins to render cells competent for DNA replication. Cdc6 is overexpressed in human cancers, where it has a critical regulatory role in addition to DNA replication. Yan et al.(1998) showed that Cdc6 is expressed selectively in proliferating but not quiescent mammalian cells, both in culture and within tissues in intact animals. During the transition from a growth-arrested to a proliferative state, transcription of mammalian Cdc6 is regulated by E2F proteins, as revealed by a functional analysis of the human Cdc6 promoter and by the ability of exogenously expressed E2F proteins to stimulate the endogenous Cdc6 gene. They conclude that expression of human Cdc6 is regulated in response to mitogenic signals though transcriptional control mechanisms involving E2F proteins, and that Cdc6 is required for initiation of DNA replication in mammalian cells.
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FAQs

Q: Is your western blot protocol provided from the website applicable for all your antibodies? Keyword: applications, WB
A: The protocol is applicable for all our antibodies in WB, the NC Membrane(0.45μm or 0.22μm) and transfer time(70 mins or 50 mins) depends on the protein molecular weight, details can be found in included protocol.
Q: Can I conjugate markers to this antibody? Can I link custom conjugates to this antibody? Keyword: conjugation
A: The antibody is stored with BSA and cannot be conjugated with markers. Carrier free antibodies are available upon request. Please contact support@bosterbio.com
Q: Can you provide monoclonal antibodies carrier free?
A: We cannot.
Q: What are some alternative names that could be used to describe this product?
A: Some common names include but are not limited to p62 antibody, cdc6 antibody