Data & Images
|Product Name||Anti-Cdk4 Antibody|
|Description||Rabbit IgG polyclonal antibody for Cyclin-dependent kinase 4(CDK4) detection. Tested with WB in Human;Mouse;Rat.|
|Cite This Product||Anti-Cdk4 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1428-1)|
|Replacement Item||This antibody may replace the following items: sc-601|sc-23896|sc-260|sc-56361|sc-749|sc-56277|sc-53636|sc-166373|sc-136241|sc-70831|sc-70832|sc-393653|sc-601-G|sc-260-G|sc-393653-X from Santa Cruz Biotechnology.|
|Validated Species||Human, Mouse, Rat|
*This antibody is predicted to react with the above species based on antigen sequence similarities. Our Boster Guarantee covers the use of this product with the above species.
*Our Boster Guarantee covers the use of this product in the above tested applications.
**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.
|Recommended Detection Systems||Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot.
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2017!
|Immunogen||A synthetic peptide corresponding to a sequence at the C-terminus of mouse Cdk4(283-303aa RISAFRALQHSYLHKEESDAE), different from the related rat sequence by one amino acid, and from the related human sequence by four amino acids.|
|Cross Reactivity||No cross reactivity with other proteins|
|Contents||Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
*carrier free antibody available upon request.
|Concentration||Add 0.2ml of distilled water will yield a concentration of 500ug/ml.|
|Storage||At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing.|
|Purification||Immunogen affinity purified.|
Protein Target Info (Source: Uniprot.org)
You can check the tissue specificity below for information on selecting positive and negative control.
|Protein Name||Cyclin-dependent kinase 4|
|Molecular Weight||33751 MW|
|Protein Function||Ser/Thr-kinase component of cyclin D-CDK4 (DC) complexes that phosphorylate and inhibit members of the retinoblastoma (RB) protein family including RB1 and regulate the cell-cycle during G(1)/S transition. Phosphorylation of RB1 allows dissociation of the transcription factor E2F from the RB/E2F complexes and the subsequent transcription of E2F target genes which are responsible for the progression through the G(1) phase. Hypophosphorylates RB1 in early G(1) phase. Cyclin D-CDK4 complexes are major integrators of various mitogenenic and antimitogenic signals. Also phosphorylates SMAD3 in a cell-cycle-dependent manner and represses its transcriptional activity. Component of the ternary complex, cyclin D/CDK4/CDKN1B, required for nuclear translocation and activity of the cyclin D-CDK4 complex (By similarity). .|
|Sequence Similarities||Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.|
|Subcellular Localization||Cytoplasm . Nucleus . Membrane . Cytoplasmic when non- complexed. Forms a cyclin D-CDK4 complex in the cytoplasm as cells progress through G(1) phase. The complex accumulates on the nuclear membrane and enters the nucleus on transition from G(1) to S phase. Also present in nucleoli and heterochromatin lumps. Colocalizes with RB1 after release into the nucleus (By similarity). .|
|Alternative Names||Cyclin-dependent kinase 4;22.214.171.124;CRK3;Cell division protein kinase 4;PSK-J3;Cdk4;Crk3;|
|Research Areas|||cell biology|cell cycle|kinases/phosphatases|cdks| epigenetics and nuclear signaling| cancer| isotype/loading controls|cdk4||
Background for Cyclin-dependent kinase 4
Dilution Ratios/Recommended Concentrations
At Boster we strive to provide the best Anti-Cdk4 Antibody by testing all applications on non-spiked tissues and cell lines to ensure that the affinity of the antibody is enough to react to the endogenouse level of the target protein. Read more about our QC panel here.
|Recommended dilution ratios are listed below:|
Western blot, 0.1-0.5μg/ml, Human, Rat, Mouse|
**Boster provides high sensitivity secondary antibody kits for Western blotting and IHC. For more info see Related Products below.
Anti-Cdk4 Antibody Images
Click the images to enlarge.
Lane 1: Rat Thymus Tissue Lysate
Lane 2: HELA Cell Lysate
Lane 3: MCF-7 Cell Lysate
Lane 4: A549 Cell Lysate
Lane 5: COLO320 Cell Lysate
Lane 6: JURKAT Cell Lysate
1. Post-translational modification:phosphorylation, methylation, glycosylation etc. These modifications prevent SDS molecules from binding to the target protein and thus make the band size appear larger than expected
2. Post-translational cleavage: this can cause smaller bands and or multiple bands
3. Alternative splicing: the same gene can have alternative splicing patterns generating different size proteins, all with reactivities to the antibody.
4. Amino Acid R chain charge: SDS binds to positive charges. The different size and charge of the Amino Acid side chains can affect the amount of SDS binding and thus affect the observed band size.
5. Multimers: Multimers are usually broken up in reducing conditions. However if the interactions between the multimers are strong, the band may appear higher.,