Anti-Cdk4 Antibody Picoband™

CDK4 antibody

Boster Bio Anti-Cdk4 Antibody Picoband™ catalog # PB9535. Tested in IF, IHC, WB applications. This antibody reacts with Human, Mouse, Rat. Cited in 10 publication(s).

Product Info Summary

SKU: PB9535
Size: 100 μg/vial
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: IF, IHC, WB

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Product Name

Anti-Cdk4 Antibody Picoband™

View all CDK4 Antibodies

SKU/Catalog Number

PB9535

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-Cdk4 Antibody Picoband™ catalog # PB9535. Tested in IF, IHC, WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Cdk4 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9535)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E.coli-derived human Cdk4 recombinant protein (Position: G201-E303). Human Cdk4 shares 94.2% amino acid (aa) sequence identity with both mouse and rat Cdk4.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins

Reactive Species

PB9535 is reactive to CDK4 in Human, Mouse, Rat

Applications

PB9535 is guaranteed for IF, IHC, WB Boster Guarantee

Observed Molecular Weight

34 kDa

Calculated molecular weight

33.73kDa

Background of CDK4

Cyclin-dependent kinase-4 (CDK4) is a protein-serine kinase involved in the cell cycle. Human cell division is regulated primarily at the G1-to-S or the G2-to-M boundaries within the cell cycle. The complexes formed by CDK4 and the D-type cyclins are involved in the control of cell proliferation during the G1 phase. CDK4 is inhibited by p16, also known as cyclin-dependent kinase inhibitor-2. CDK4 is mapped to 12q14. And CDK4 expression and activity are required for cytokine responsiveness in T cells.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Mouse, Rat, By Heat
Immunofluorescence, 5 μg/ml, Human

Validation Images & Assay Conditions

Gene/Protein Information For CDK4 (Source: Uniprot.org, NCBI)

Gene Name

CDK4

Full Name

Cyclin-dependent kinase 4

Weight

33.73kDa

Superfamily

protein kinase superfamily

Alternative Names

CDK4; Cell division protein kinase 4; CMM3; cyclin-dependent kinase 4; EC 2.7.11; EC 2.7.11.22; melanoma cutaneous malignant, 3; MGC14458; PSK-J3; PSK-J3cell division kinase 4 CDK4 CMM3, PSK-J3 cyclin dependent kinase 4 cyclin-dependent kinase 4|cell division protein kinase 4

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on CDK4, check out the CDK4 Infographic

CDK4 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for CDK4: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

Hello CJ!

PB9535 has been cited in 10 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Effect of Bone Morphogenetic Protein-2 on Proliferation and Apoptosis of Gastric Cancer Cells

Platycodin D, a metabolite of Platycodin grandiflorum, inhibits highly metastatic MDA-MB-231 breast cancer growth in vitro and in vivo by targeting the MDM2 oncogene

Combined Anti-Cancer Effects of Platycodin D and Sorafenib on Androgen-Independent and PTEN-Deficient Prostate Cancer

Dynamic changes of cell cycle elements in the ischemic brain after bone marrow stromal cells transplantation in rats

Glucagon-like peptide 1 treatment reverses vascular remodelling by downregulating matrix metalloproteinase 1 expression through inhibition of the ERK1/2/NF-κB signalling pathway

OAMDP, a novel podophyllotoxin derivative, induces apoptosis, cell cycle arrest and autophagy in hepatoma HepG2 cells

Lu Kong,Yongya Wu,Wangcheng Hu,Lin Liu,Yuying Xue,Geyu Liang,Mechanisms underlying reproductive toxicity induced by nickel nanoparticles identified by comprehensive gene expression analysis in GC-1 spg cells,Environmental Pollution,2021,116556,ISSN 0269-7
Species: Mouse
PB9535 usage in article: APP:WB, SAMPLE:GC-1 CELL, DILUTION:1:1000

Effect of Bone Morphogenetic Protein-2 on Proliferation and Apoptosis of Gastric Cancer Cells

Deng L, Lu Y, Zhao X, Sun Y, Shi Y, Fan H, Liu C, Zhou J, Nie Y, Wu K, Fan D, Guo X. Biochem Biophys Res Commun. 2013 Oct 18;440(2):322-9. Doi: 10.1016/J.Bbrc.2013.09.079. Epub 2013 Sep 25. Ran Gtpase Protein Promotes Human Pancreatic Cancer Proli...

Liu W, Dai Q, Lu N, Wei L, Ha J, Rong J, Mu R, You Q, Li Z, Guo Q. Biochem Cell Biol. 2011 Jun;89(3):287-98. Doi: 10.1139/O10-162. Epub 2011 Apr 14. Lyg-202 Inhibits The Proliferation Of Human Colorectal Carcinoma Hct-116 Cells Through Induction O...

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5 Customer Q&As for Anti-Cdk4 Antibody Picoband™

Question

We are currently using anti-Cdk4 antibody PB9535 for mouse tissue, and we are satisfied with the IHC results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on zebrafish tissues as well?

Verified Customer

Verified customer

Asked: 2020-03-09

Answer

The anti-Cdk4 antibody (PB9535) has not been validated for cross reactivity specifically with zebrafish tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in zebrafish you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2020-03-09

Question

We have seen staining in rat embryo. Any tips? Is anti-Cdk4 antibody supposed to stain embryo positively?

Verified Customer

Verified customer

Asked: 2019-09-19

Answer

Based on literature embryo does express CDK4. Based on Uniprot.org, CDK4 is expressed in right ovary, embryo, muscle, among other tissues. Regarding which tissues have CDK4 expression, here are a few articles citing expression in various tissues:
Embryo, Pubmed ID: 14702039
Muscle, Pubmed ID: 15489334

Boster Scientific Support

Answered: 2019-09-19

Question

I am interested in using your anti-Cdk4 antibody for regulation of insulin receptor signaling pathway studies. Has this antibody been tested with western blotting on mouse intestine tissue? We would like to see some validation images before ordering.

Verified Customer

Verified customer

Asked: 2019-06-25

Answer

We appreciate your inquiry. This PB9535 anti-Cdk4 antibody is validated on rat thymus tissue, gaster tissue, tissue lysate, hela whole cell lysate, a549 whole cell lysate, skov whole cell lysate, 22rv1 whole cell lysate, mouse intestine tissue, lung cancer tissue. It is guaranteed to work for IHC, WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.

Boster Scientific Support

Answered: 2019-06-25

Question

We ordered your anti-Cdk4 antibody for IHC on muscle in the past. I am using human, and We are going to use the antibody for WB next. I would like examining muscle as well as right ovary in our next experiment. Could give a recommendation on which antibody would work the best for WB?

K. Wu

Verified customer

Asked: 2017-03-27

Answer

I looked at the website and datasheets of our anti-Cdk4 antibody and it seems that PB9535 has been validated on human in both IHC and WB. Thus PB9535 should work for your application. Our Boster satisfaction guarantee will cover this product for WB in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for WB detection and you can check out our website bosterbio.com to find out more information about them.

Boster Scientific Support

Answered: 2017-03-27

Question

Our team were happy with the WB result of your anti-Cdk4 antibody. However we have seen positive staining in right ovary cytoplasm. using this antibody. Is that expected? Could you tell me where is CDK4 supposed to be expressed?

R. Zhao

Verified customer

Asked: 2013-06-13

Answer

From what I have seen in literature, right ovary does express CDK4. Generally CDK4 expresses in cytoplasm. Regarding which tissues have CDK4 expression, here are a few articles citing expression in various tissues:
Embryo, Pubmed ID: 14702039
Muscle, Pubmed ID: 15489334

Boster Scientific Support

Answered: 2013-06-13

Order DetailsPrice
PB9535

100μg

$370
PB9535-10ug

10μg sample (liquid)

$99
PB9535-Biotin

100 μg Biotin conjugated

$570
PB9535-Cy3

100 μg Cy3 conjugated

$570
PB9535-Dylight488

100 μg Dylight488 conjugated

$570
PB9535-Dylight550

100 μg Dylight550 conjugated

$570
PB9535-Dylight594

100 μg Dylight594 conjugated

$570
PB9535-FITC

100 μg FITC conjugated

$570
PB9535-HRP

100 μg HRP conjugated

$570
PB9535-APC

100 μg APC conjugated

$670
PB9535-PE

100 μg PE conjugated

$670
PB9535-carrier-free

Carrier Free

$370

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