Product Info Summary
SKU: | M00511 |
---|---|
Size: | 100 μl |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | Flow Cytometry, IP, IHC, WB |
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Product info
Product Name
Anti-CDK5 Rabbit Monoclonal Antibody
SKU/Catalog Number
M00511
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-CDK5 Rabbit Monoclonal Antibody catalog # M00511. Tested in WB, IHC, IP, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-CDK5 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00511)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
DAH-3
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human Cdk5
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
Detects ~20kDa. Does not cross-react with αB-crystallin, βL-crystallin, ΒH- crystallin, γ-crystallin, HSP25, HSP27 or HSP47 proteins.
Reactive Species
M00511 is reactive to CDK5 in Human, Mouse, Rat
Applications
M00511 is guaranteed for Flow Cytometry, IP, IHC, WB Boster Guarantee
Observed Molecular Weight
30 kDa
Calculated molecular weight
33.304kDa
Background of CDK5
Dynamin-related GTPase required for mitochondrial fusion and regulation of apoptosis. May form a diffusion barrier for proteins stored in mitochondrial cristae. Proteolytic processing in response to intrinsic apoptotic signals may lead to disassembly of OPA1 oligomers and release of the caspase activator cytochrome C (CYCS) into the mitochondrial intermembrane space.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Innovating Scientists Reward
If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.
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Assay dilution & Images
Reconsitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:1000-1:5000
IHC 1:50-1:200
IP 1:50
FC 1:50
Validation Images & Assay Conditions
Click image to see more details
Western blot analysis of CDK5 expression in HeLa cell lysate.
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Immunohistochemical analysis of paraffin-embedded Human esophageal carcinoma, using the Antibody at 1:200 dilution.
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Immunohistochemical analysis of paraffin-embedded Rat stomach, using the Antibody at 1:200 dilution.
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Immunohistochemical analysis of paraffin-embedded Human stomach, using the Antibody at 1:200 dilution.
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Immunohistochemical analysis of paraffin-embedded Rat heart, using the Antibody at 1:200 dilution.
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Immunohistochemical analysis of paraffin-embedded Human prostate cancer, using the Antibody at 1:200 dilution.
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Immunohistochemical analysis of paraffin-embedded human kidney, using CDK5 Antibody .
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Immunofluorescent analysis using the Antibody at 1:50 dilution.
Protein Target Info & Infographic
Gene/Protein Information For CDK5 (Source: Uniprot.org, NCBI)
Gene Name
CDK5
Full Name
Cyclin-dependent-like kinase 5
Weight
33.304kDa
Superfamily
protein kinase superfamily
Alternative Names
CDK5; CDKN5; Cell division protein kinase 5; cyclin-dependent kinase 5; EC 2.7.11; EC 2.7.11.22; LIS7; protein kinase CDK5 splicing; PSSALRE; Serine/threonine-protein kinase PSSALRE; Tau protein kinase II catalytic subunit; TPKII catalytic subunit CDK5 LIS7, PSSALRE cyclin dependent kinase 5 cyclin-dependent-like kinase 5|TPKII catalytic subunit|cell division protein kinase 5|epididymis secretory sperm binding protein|protein kinase CDK5 splicing|serine/threonine-protein kinase PSSALRE|tau protein kinase II catalytic subunit
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CDK5, check out the CDK5 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CDK5: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-CDK5 Rabbit Monoclonal Antibody (M00511)
Hello CJ!
M00511 has been cited in 1 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
A new treatment for cognitive disorders related toin uteroexposure to alcohol
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Customer Reviews
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Customer Q&As
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6 Customer Q&As for Anti-CDK5 Rabbit Monoclonal Antibody
Question
Does anti-CDK5 Rabbit Monoclonal antibody M00511 work on feline WB with cervix carcinoma erythroleukemia?
Verified Customer
Verified customer
Asked: 2019-12-16
Answer
Our lab technicians have not tested anti-CDK5 Rabbit Monoclonal antibody M00511 on feline. You can run a BLAST between feline and the immunogen sequence of anti-CDK5 Rabbit Monoclonal antibody M00511 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated feline samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in feline cervix carcinoma erythroleukemia in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-12-16
Question
I see that the anti-CDK5 Rabbit Monoclonal antibody M00511 works with IP, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-11-15
Answer
You can find protocols for IP on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-11-15
Question
Is this M00511 anti-CDK5 Rabbit Monoclonal antibody reactive to the isotypes of CDK5?
H. Krishna
Verified customer
Asked: 2019-09-10
Answer
The immunogen of M00511 anti-CDK5 Rabbit Monoclonal antibody is A synthesized peptide derived from human Cdk5 . Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-09-10
Question
Is a blocking peptide available for product anti-CDK5 Rabbit Monoclonal antibody (M00511)?
Verified Customer
Verified customer
Asked: 2019-06-04
Answer
We do provide the blocking peptide for product anti-CDK5 Rabbit Monoclonal antibody (M00511). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2019-06-04
Question
I was wanting to use your anti-CDK5 Rabbit Monoclonal antibody for IP for rat testis on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for rat testis identification?
Verified Customer
Verified customer
Asked: 2017-05-25
Answer
As indicated on the product datasheet, M00511 anti-CDK5 Rabbit Monoclonal antibody has been validated for IP, IHC, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in rat testis in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2017-05-25
Question
I have a question about product M00511, anti-CDK5 Rabbit Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
V. Li
Verified customer
Asked: 2015-08-24
Answer
We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M00511 anti-CDK5 Rabbit Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2015-08-24