Data & Images
|Product Name||Anti-CEACAM1/Cd66A Monoclonal Antibody|
|Description||Mouse IgG monoclonal antibody for CEA, carcinoembryonic antigen-related cell adhesion molecule 1 (CEACAM1) detection. Tested with WB, IHC-P in Human. No cross reactivity with other proteins.|
|Cite This Product||Anti-CEACAM1/Cd66A Monoclonal Antibody C6G9 (Boster Biological Technology, Pleasanton CA, USA, Catalog # MA1023)|
|Replacement Item||This antibody may replace the following items: sc-166453|sc-8226|sc-25627|sc-59812|sc-166404|sc-8222|sc-70450|sc-8224 from Santa Cruz Biotechnology.|
*Our Boster Guarantee covers the use of this product in the above tested applications.
**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.
|Recommended Detection Systems||Boster recommends Enhanced Chemiluminescent Kit with anti-Mouse IgG (EK1001) for Western blot, and HRP Conjugated anti-Mouse IgG Super Vision Assay Kit (SV0001-1) for IHC(P).
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2017!
|Immunogen||Carcinoembryonic antigen(CEA) isolated from a human colon adenocarcinoma cell line.|
|Cross Reactivity||No cross reactivity with other proteins|
|Contents||Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.
*carrier free antibody available upon request.
|Concentration||Add 1ml of PBS buffer will yield a concentration of 100ug/ml.|
|Storage||At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.|
Protein Target Info (Source: Uniprot.org)
You can check the tissue specificity below for information on selecting positive and negative control.
|Protein Name||Carcinoembryonic antigen-related cell adhesion molecule 1|
|Molecular Weight||57264 MW|
|Protein Function||This protein is a calcium-independent cell adhesion molecule with homophilic binding properties. May play a role in the formation and maintenance of the specialized membrane structure of the apical surface of the hepatocytes.|
|Tissue Specificity||Expressed in epithelia, vessel endothelia, leukocytes and platelets.|
|Sequence Similarities||Belongs to the immunoglobulin superfamily. CEA family.|
|Subcellular Localization||Cell membrane; Single-pass type I membrane protein. Canalicular domain of hepatocyte plasma membranes.|
|Alternative Names||Carcinoembryonic antigen-related cell adhesion molecule 1;ATP-dependent taurocolate-carrier protein;Cell-CAM 105;C-CAM 105;Ecto-ATPase;GP110;pp120;CD66a;Ceacam1;|
|Research Areas|||tags & cell markers|cell type markers|tumor associated| cancer|tumor immunology|tumor-associated antigens|oncoproteins/suppressors|tumor suppressors|tumor biomarkers|tumor antigens| microbiology|protein|human protein|receptor||
Background for Carcinoembryonic antigen-related cell adhesion molecule 1
Dilution Ratios/Recommended Concentrations
At Boster we strive to provide the best Anti-CEACAM1/Cd66A Monoclonal Antibody by testing all applications on non-spiked tissues and cell lines to ensure that the affinity of the antibody is enough to react to the endogenouse level of the target protein. Read more about our QC panel here.
|Recommended dilution ratios are listed below:|
Immunohistochemistry(Paraffin-embedded Section), 0.5-2μg/ml, Human, By Heat
Western blot, 0.5-1μg/ml, Human
**Boster provides high sensitivity secondary antibody kits for Western blotting and IHC. For more info see Related Products below.
Anti-CEACAM1/Cd66A Monoclonal Antibody Images
Click the images to enlarge.
Lane 1: Recombinant Human CEA Protein 10ng
Lane 2: Recombinant Human CEA Protein 5ng
Lane 3: Recombinant Human CEA Protein 2
IHC(P): Human Intestinal Cancer Tissue
IHC(P): Human Intestinal Cancer Tissue
IHC(P): Human Rectal Cancer Tissue