SKU MA1023
Size 100μg/vial
Reactivity Human
Clonality Monoclonal
Clone Number C6G9
Host Mouse
Ig Isotype Mouse IgG1
Applications IHC, WB

Overview

Product Name Anti-CEACAM1/Cd66A Monoclonal Antibody
SKU/Catalog Number MA1023
Storage & Handling At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.
Size 100μg/vial
Description Mouse IgG monoclonal antibody for CEA, carcinoembryonic antigen-related cell adhesion molecule 1 (CEACAM1) detection. Tested with WB, IHC-P in Human. No cross reactivity with other proteins.
Cite This Product Anti-CEACAM1/Cd66A Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # MA1023)
Host Mouse
Immunogen Carcinoembryonic antigen(CEA) isolated from a human colon adenocarcinoma cell line.
Reactivity Human

Assay Details

Assay Dilutions Overview

Immunohistochemistry(Paraffin-embedded Section), 0.5-2μg/ml, Human, By Heat
Western blot, 0.5-1μg/ml, Human

Boster's Secondary Antibodies And IHC, WB Kits

The following reagents are used to generate the images below.

Boster recommends Enhanced Chemiluminescent Kit with anti-Mouse IgG (EK1001) for Western blot, and HRP Conjugated anti-Mouse IgG Super Vision Assay Kit (SV0001-1) for IHC(P).

Images And Assay Conditions

/antibody/ma1023 1 WB anti ceacam1 cd66a antibody.jpg

Anti-CEA antibody (monoclonal), MA1023, Western blotting
Lane 1: Recombinant Human CEA Protein 10ng
Lane 2: Recombinant Human CEA Protein 5ng
Lane 3: Recombinant Human CEA Protein 2

/antibody/ma1023 2 IHC anti ceacam1 cd66a antibody.jpg

Anti-CEA antibody (monoclonal), MA1023, IHC(P)
IHC(P): Human Intestinal Cancer Tissue

/antibody/ma1023 3 IHC anti ceacam1 cd66a antibody.jpg

Anti-CEA antibody (monoclonal), MA1023, IHC(P)
IHC(P): Human Intestinal Cancer Tissue

/antibody/ma1023 4 IHC anti ceacam1 cd66a antibody.jpg

Anti-CEA antibody (monoclonal), MA1023, IHC(P)
IHC(P): Human Rectal Cancer Tissue

Target Info

Protein Target Info (Source: Uniprot.org)

Uniprot Id P16573
Gene Name Ceacam1
Protein Name Carcinoembryonic antigen-related cell adhesion molecule 1
Tissue Specificity Expressed in epithelia, vessel endothelia, leukocytes and platelets.
Alternative Names Carcinoembryonic antigen-related cell adhesion molecule 1;ATP-dependent taurocolate-carrier protein;Cell-CAM 105;C-CAM 105;Ecto-ATPase;GP110;pp120;CD66a;Ceacam1;
Subcellular Localization Cell membrane; Single-pass type I membrane protein. Canalicular domain of hepatocyte plasma membranes.
Molecular Weight 57264 MW

*if product is indicated to react with multiple species, protein info is based on the human gene.

Ontology

Protein Function This protein is a calcium-independent cell adhesion molecule with homophilic binding properties. May play a role in the formation and maintenance of the specialized membrane structure of the apical surface of the hepatocytes.
Background Carcinoembryonic antigen is a complex immunoreactive glycoprotein with a molecular weight of 180,000 comprising 60% carbohydrate. It is found in adenocarcinomas of endodermally derived digestive system epithelia and in fetal colon.Carcinoembryonic antigen is one of the most widely used tumor markers in serum immunoassay determinations of carcinoma.

Order Product (MA1023)

Promotion:

Buy primary get secondary antibody for free.
Option Price
30ug sample size $99
100ug $280
100ug+Free HRP Secondary BA1050 $280
100ug+Free Biotin Secondary BA1001 $280
Ships in 5-7 business days.

Troubleshooting

Download troubleshooting handbooks for IHC, Western blot and ELISA for FREE.

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Guaranteed product quality

We promise all of our products perform as described in datasheets.

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Publications

Li H, Chen L, Zeng S, Li X, Zhang X, Lin C, Zhang M, Xie S, He Y, Shu S, Yang L, Tang S, Fu X. Exp Cell Res. 2015 Mar 1;332(1):67-77. Doi: 10.1016/J.Yexcr.2015.01.014. Epub 2015 Jan 30. Matrigel Basement Membrane Matrix Induces Eccrine Sweat Gland...
Hu C, Zheng J, Su X, Wang J, Wu W, Hu S. Anal Chem. 2013 Nov 5;85(21):10612-9. Doi: 10.1021/Ac4028005. Epub 2013 Oct 11. Ultrasensitive All-Carbon Photoelectrochemical Bioprobes For Zeptomole Immunosensing Of Tumor Markers By An Inexpensive Visibl...

Customer Q&As

Q: Is your western blot protocol provided from the website applicable for all your antibodies? Keyword: applications, WB
A: The protocol is applicable for all our antibodies in WB, the NC Membrane(0.45μm or 0.22μm) and transfer time(70 mins or 50 mins) depends on the protein molecular weight, details can be found in included protocol.
Q: Can I conjugate markers to this antibody? Can I link custom conjugates to this antibody? Keyword: conjugation
A: The antibody is stored with BSA and cannot be conjugated with markers. Carrier free antibodies are available upon request. Please contact support@bosterbio.com
Q: Can you provide monoclonal antibodies carrier free?
A: We cannot.
Q: What are some alternative names that could be used to describe this product?
A: Some common names include but are not limited to cea antibody, carcinoembryonic antigen antibody