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SKU:MA1023
Pack Size:100μg/vial
Sample Size:30ug for $99, contact us for details
Clonality:Monoclonal
Clone Number:C6G9
Application:IHC-P, WB
Price: $200.00
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Overview

Product Name Anti-CEACAM1/Cd66A Antibody
SKU/Catalog Number MA1023
Description Mouse IgG monoclonal antibody for CEA, carcinoembryonic antigen-related cell adhesion molecule 1 (CEACAM1) detection. Tested with WB, IHC-P in Human. No cross reactivity with other proteins.
Cite This Product Anti-CEACAM1/Cd66A Antibody C6G9 (Boster Biological Technology, Pleasanton CA, USA, Catalog # MA1023)
Replacement Item This antibody may replace the following items: sc-166453|sc-8226|sc-25627|sc-59812|sc-166404|sc-8222|sc-70450|sc-8224 from Santa Cruz Biotechnology.
Host Mouse
Isotype Mouse IgG1
Validated Species Human
Application IHC-P, WB

*Our Boster Guarantee covers the use of this product in the above tested applications.

**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.

Recommended Detection Systems Boster recommends Enhanced Chemiluminescent Kit with anti-Mouse IgG (EK1001) for Western blot, and HRP Conjugated anti-Mouse IgG Super Vision Assay Kit (SV0001-1) for IHC(P).
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2017!
Immunogen Carcinoembryonic antigen(CEA) isolated from a human colon adenocarcinoma cell line.
Cross Reactivity No cross reactivity with other proteins
Pack Size 100μg/vial

Properties

Clonality Monoclonal
Clone Number C6G9
Form lyophilized
Contents Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.
*carrier free antibody available upon request.
Concentration Add 1ml of PBS buffer will yield a concentration of 100ug/ml.
Storage At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time. Avoid repeated freezing and thawing.
Purification Ascites
Isotype Mouse IgG1

Protein Target Info (Source: Uniprot.org)

You can check the tissue specificity below for information on selecting positive and negative control.

Gene Name Ceacam1
Protein Name Carcinoembryonic antigen-related cell adhesion molecule 1
Molecular Weight 57264 MW
Protein Function This protein is a calcium-independent cell adhesion molecule with homophilic binding properties. May play a role in the formation and maintenance of the specialized membrane structure of the apical surface of the hepatocytes.
Tissue Specificity Expressed in epithelia, vessel endothelia, leukocytes and platelets.
Sequence Similarities Belongs to the immunoglobulin superfamily. CEA family.
Subcellular Localization Cell membrane; Single-pass type I membrane protein. Canalicular domain of hepatocyte plasma membranes.
Uniprot ID P16573
Alternative Names Carcinoembryonic antigen-related cell adhesion molecule 1;ATP-dependent taurocolate-carrier protein;Cell-CAM 105;C-CAM 105;Ecto-ATPase;GP110;pp120;CD66a;Ceacam1;
Research Areas |tags & cell markers|cell type markers|tumor associated| cancer|tumor immunology|tumor-associated antigens|oncoproteins/suppressors|tumor suppressors|tumor biomarkers|tumor antigens| microbiology|protein|human protein|receptor|
*if product is indicated to react with multiple species, protein info is based on the human gene.

Background for Carcinoembryonic antigen-related cell adhesion molecule 1

Carcinoembryonic antigen is a complex immunoreactive glycoprotein with a molecular weight of 180,000 comprising 60% carbohydrate. It is found in adenocarcinomas of endodermally derived digestive system epithelia and in fetal colon.Carcinoembryonic antigen is one of the most widely used tumor markers in serum immunoassay determinations of carcinoma.

Anti-CEACAM1/Cd66A Antibody Images

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Anti-CEACAM1/Cd66A Antibody
Anti-CEA antibody (monoclonal), MA1023, Western blotting
Lane 1: Recombinant Human CEA Protein 10ng
Lane 2: Recombinant Human CEA Protein 5ng
Lane 3: Recombinant Human CEA Protein 2
Anti-CEACAM1/Cd66A Antibody
Anti-CEA antibody (monoclonal), MA1023, IHC(P)
IHC(P): Human Intestinal Cancer Tissue
Anti-CEACAM1/Cd66A Antibody
Anti-CEA antibody (monoclonal), MA1023, IHC(P)
IHC(P): Human Intestinal Cancer Tissue
Anti-CEACAM1/Cd66A Antibody
Anti-CEA antibody (monoclonal), MA1023, IHC(P)
IHC(P): Human Rectal Cancer Tissue
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Publications

Li H, Chen L, Zeng S, Li X, Zhang X, Lin C, Zhang M, Xie S, He Y, Shu S, Yang L, Tang S, Fu X. Exp Cell Res. 2015 Mar 1;332(1):67-77. Doi: 10.1016/J.Yexcr.2015.01.014. Epub 2015 Jan 30. Matrigel Basement Membrane Matrix Induces Eccrine Sweat Gland...
Hu C, Zheng J, Su X, Wang J, Wu W, Hu S. Anal Chem. 2013 Nov 5;85(21):10612-9. Doi: 10.1021/Ac4028005. Epub 2013 Oct 11. Ultrasensitive All-Carbon Photoelectrochemical Bioprobes For Zeptomole Immunosensing Of Tumor Markers By An Inexpensive Visibl...

FAQs

Q: Is your western blot protocol provided from the website applicable for all your antibodies? Keyword: applications, WB
A: The protocol is applicable for all our antibodies in WB, the NC Membrane(0.45μm or 0.22μm) and transfer time(70 mins or 50 mins) depends on the protein molecular weight, details can be found in included protocol.
Q: Can I conjugate markers to this antibody? Can I link custom conjugates to this antibody? Keyword: conjugation
A: The antibody is stored with BSA and cannot be conjugated with markers. Carrier free antibodies are available upon request. Please contact support@bosterbio.com
Q: Can you provide monoclonal antibodies carrier free?
A: We cannot.
Q: What are some alternative names that could be used to describe this product?
A: Some common names include but are not limited to cea antibody, carcinoembryonic antigen antibody