Product Info Summary
SKU: | M01100-1 |
---|---|
Size: | 100 μl |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | Flow Cytometry, IP, WB |
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Product info
Product Name
Anti-CEBP Beta Rabbit Monoclonal Antibody
SKU/Catalog Number
M01100-1
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-CEBP Beta Rabbit Monoclonal Antibody catalog # M01100-1. Tested in WB, IP, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-CEBP Beta Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01100-1)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
AAG-3
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human CEBP Beta
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
Detects ~20kDa. Does not cross-react with αB-crystallin, βL-crystallin, ΒH- crystallin, γ-crystallin, HSP25, HSP27 or HSP47 proteins.
Reactive Species
M01100-1 is reactive to CEBPB in Human, Mouse, Rat
Applications
M01100-1 is guaranteed for Flow Cytometry, IP, WB Boster Guarantee
Observed Molecular Weight
110 kDa
Calculated molecular weight
36.106kDa
Background of CEBP Beta
Activated by icilin, eucalyptol, menthol, cold and modulation of intracellular pH. Involved in menthol sensation. Permeable for monovalent cations sodium, potassium, and cesium and divalent cation calcium.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Innovating Scientists Reward
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Assay dilution & Images
Reconsitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IP 1:50
FC 1:50
Validation Images & Assay Conditions
Click image to see more details
Western blot analysis of CEBP Beta in (1) PC-12 cell lysate; (2) Human spleen lysate.
Protein Target Info & Infographic
Gene/Protein Information For CEBPB (Source: Uniprot.org, NCBI)
Gene Name
CEBPB
Full Name
CCAAT/enhancer-binding protein beta
Weight
36.106kDa
Superfamily
bZIP family
Alternative Names
C/EBP beta; C/EBP-beta; CCAAT/enhancer binding protein (C/EBP), beta; CCAAT/enhancer-binding protein beta; CRP2; IL6DBP; interleukin 6-dependent DNA-binding protein; LAPTCF-5; Liver activator protein; liver-enriched transcriptional activator protein; MGC32080; NF-IL6; Nuclear factor NF-IL6; nuclear factor of interleukin 6; TCF5NFIL6; Transcription factor 5 CEBPB C/EBP-beta, IL6DBP, NF-IL6, TCF5 CCAAT enhancer binding protein beta CCAAT/enhancer-binding protein beta|CCAAT/enhancer binding protein (C/EBP), beta|interleukin 6-dependent DNA-binding protein|nuclear factor NF-IL6|nuclear factor of interleukin 6|transcription factor 5|transcription factor C/EBP beta
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CEBPB, check out the CEBPB Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CEBPB: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-CEBP Beta Rabbit Monoclonal Antibody (M01100-1)
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No publications found for M01100-1
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Customer Reviews
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Customer Q&As
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3 Customer Q&As for Anti-CEBP Beta Rabbit Monoclonal Antibody
Question
I was wanting to use your anti-CEBP Beta Rabbit Monoclonal antibody for IP for mouse muscle skin on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for mouse muscle skin identification?
G. Parker
Verified customer
Asked: 2019-11-05
Answer
You can see on the product datasheet, M01100-1 anti-CEBP Beta Rabbit Monoclonal antibody has been tested for IP, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in mouse muscle skin in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-11-05
Question
I have a question about product M01100-1, anti-CEBP Beta Rabbit Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-10-02
Answer
We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M01100-1 anti-CEBP Beta Rabbit Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-10-02
Question
Does M01100-1 anti-CEBP Beta Rabbit Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-09-10
Answer
As indicated on the product datasheet, M01100-1 anti-CEBP Beta Rabbit Monoclonal antibody as been tested on IP. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-09-10