Anti-CEBP Beta Rabbit Monoclonal Antibody

CEBP Beta antibody

Boster Bio Anti-CEBP Beta Rabbit Monoclonal Antibody catalog # M01100-1. Tested in WB, IP, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.

Product Info Summary

SKU: M01100-1
Size: 100 μl
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: Flow Cytometry, IP, WB

Product Name

Anti-CEBP Beta Rabbit Monoclonal Antibody

View all CEBP Beta Antibodies

SKU/Catalog Number

M01100-1

Size

100 μl

Form

Liquid

Description

Boster Bio Anti-CEBP Beta Rabbit Monoclonal Antibody catalog # M01100-1. Tested in WB, IP, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-CEBP Beta Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01100-1)

Host

Rabbit

Contents

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.

Clonality

Monoclonal

Clone Number

AAG-3

Isotype

Rabbit IgG

Immunogen

A synthesized peptide derived from human CEBP Beta

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

Detects ~20kDa. Does not cross-react with αB-crystallin, βL-crystallin, ΒH- crystallin, γ-crystallin, HSP25, HSP27 or HSP47 proteins.

Reactive Species

M01100-1 is reactive to CEBPB in Human, Mouse, Rat

Applications

M01100-1 is guaranteed for Flow Cytometry, IP, WB Boster Guarantee

Observed Molecular Weight

110 kDa

Calculated molecular weight

36.106kDa

Background of CEBP Beta

Activated by icilin, eucalyptol, menthol, cold and modulation of intracellular pH. Involved in menthol sensation. Permeable for monovalent cations sodium, potassium, and cesium and divalent cation calcium.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB 1:500-1:2000
IP 1:50
FC 1:50

Validation Images & Assay Conditions

Gene/Protein Information For CEBPB (Source: Uniprot.org, NCBI)

Gene Name

CEBPB

Full Name

CCAAT/enhancer-binding protein beta

Weight

36.106kDa

Superfamily

bZIP family

Alternative Names

C/EBP beta; C/EBP-beta; CCAAT/enhancer binding protein (C/EBP), beta; CCAAT/enhancer-binding protein beta; CRP2; IL6DBP; interleukin 6-dependent DNA-binding protein; LAPTCF-5; Liver activator protein; liver-enriched transcriptional activator protein; MGC32080; NF-IL6; Nuclear factor NF-IL6; nuclear factor of interleukin 6; TCF5NFIL6; Transcription factor 5 CEBPB C/EBP-beta, IL6DBP, NF-IL6, TCF5 CCAAT enhancer binding protein beta CCAAT/enhancer-binding protein beta|CCAAT/enhancer binding protein (C/EBP), beta|interleukin 6-dependent DNA-binding protein|nuclear factor NF-IL6|nuclear factor of interleukin 6|transcription factor 5|transcription factor C/EBP beta

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on CEBPB, check out the CEBPB Infographic

CEBPB infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for CEBPB: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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3 Customer Q&As for Anti-CEBP Beta Rabbit Monoclonal Antibody

Question

I was wanting to use your anti-CEBP Beta Rabbit Monoclonal antibody for IP for mouse muscle skin on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for mouse muscle skin identification?

G. Parker

Verified customer

Asked: 2019-11-05

Answer

You can see on the product datasheet, M01100-1 anti-CEBP Beta Rabbit Monoclonal antibody has been tested for IP, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in mouse muscle skin in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2019-11-05

Question

I have a question about product M01100-1, anti-CEBP Beta Rabbit Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

Verified Customer

Verified customer

Asked: 2019-10-02

Answer

We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M01100-1 anti-CEBP Beta Rabbit Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2019-10-02

Question

Does M01100-1 anti-CEBP Beta Rabbit Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

Verified Customer

Verified customer

Asked: 2019-09-10

Answer

As indicated on the product datasheet, M01100-1 anti-CEBP Beta Rabbit Monoclonal antibody as been tested on IP. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2019-09-10

Order DetailsPrice
M01100-1

100μL

$370
M01100-1-30ul

30μL sample

$99
M01100-1-carrier-free

Carrier Free

$370

More conjugates/formats

Free Secondary Antibody

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Eddy test
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M01100-1
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00

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