Anti-CHML/Rep 2 Antibody

CHML antibody

Boster Bio Anti-CHML/Rep 2 Antibody (Catalog # A08815). Tested in WB, IHC applications. This antibody reacts with Human.

Product Info Summary

SKU: A08815
Size: 100 µl
Reactive Species: Human
Host: Rabbit
Application: IHC, WB

Product Name

Anti-CHML/Rep 2 Antibody

View all CHML Antibodies

SKU/Catalog Number

A08815

Size

100 µl

Form

Liquid

Description

Boster Bio Anti-CHML/Rep 2 Antibody (Catalog # A08815). Tested in WB, IHC applications. This antibody reacts with Human.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-CHML/Rep 2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A08815)

Host

Rabbit

Contents

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Clonality

Polyclonal

Isotype

IgG

Immunogen

Synthesized peptide derived from internal of human CHML.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

A08815 is reactive to CHML in Human

Applications

A08815 is guaranteed for IHC, WB Boster Guarantee

Observed Molecular Weight

Calculated molecular weight

74.071kDa

Background of CHML

Substrate-binding subunit (component A) of the Rab geranylgeranyltransferase (GGTase) complex. Binds unprenylated Rab proteins and presents the substrate peptide to the catalytic component B. The component A is thought to be regenerated by transferring its prenylated Rab back to the donor membrane. Less effective than CHM in supporting prenylation of Rab3 family.

Cremers F.P.M., Hum. Mol. Genet. 1:71-75(1992).
Kasper G., Gene 295:27-32(2002).
Ota T. Nat. Genet. 36:40-45(2004)

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blotting: 1:500~1:3000
Immunohistochemistry: 1:50~1:100

Validation Images & Assay Conditions

Gene/Protein Information For CHML (Source: Uniprot.org, NCBI)

Gene Name

CHML

Full Name

Rab proteins geranylgeranyltransferase component A 2

Weight

74.071kDa

Superfamily

Rab GDI family

Alternative Names

Choroideraemia-like protein; choroideremia-like (Rab escort protein 2); FLJ10071; Rab escort protein 2; rab proteins geranylgeranyltransferase component A 2; REP-2; REP-2, Rab escort protein 2; REP2FLJ13361 CHML REP2 CHM like Rab escort protein rab proteins geranylgeranyltransferase component A 2|CHM like Rab escort protein 2|choroideraemia-like protein|choroideremia-like (Rab escort protein 2)|choroideremia-like protein

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on CHML, check out the CHML Infographic

CHML infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for CHML: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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5 Customer Q&As for Anti-CHML/Rep 2 Antibody

Question

See below the WB image, lot number and protocol we used for frontal cortex using anti-CHML/Rep 2 antibody A08815. Please let me know if you require anything else.

Verified Customer

Verified customer

Asked: 2020-04-13

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2020-04-13

Question

Is this A08815 anti-CHML/Rep 2 antibody reactive to the isotypes of CHML?

Verified Customer

Verified customer

Asked: 2019-11-13

Answer

The immunogen of A08815 anti-CHML/Rep 2 antibody is Synthesized peptide derived from internal of human CHML. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2019-11-13

Question

Would A08815 anti-CHML/Rep 2 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

Verified Customer

Verified customer

Asked: 2019-08-05

Answer

As indicated on the product datasheet, A08815 anti-CHML/Rep 2 antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2019-08-05

Question

Is there a BSA free version of anti-CHML/Rep 2 antibody A08815 available?

G. Moore

Verified customer

Asked: 2018-03-08

Answer

We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-CHML/Rep 2 antibody A08815 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2018-03-08

Question

We are currently using anti-CHML/Rep 2 antibody A08815 for human tissue, and we are well pleased with the IHC results. The species of reactivity given in the datasheet says human. Is it true that the antibody can work on monkey tissues as well?

N. Patel

Verified customer

Asked: 2014-08-04

Answer

The anti-CHML/Rep 2 antibody (A08815) has not been tested for cross reactivity specifically with monkey tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in monkey you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2014-08-04

Order DetailsPrice
A08815-100uL

100uL

$360

Free Secondary Antibody

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A08815
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$360.00

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