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Product Info Summary
| SKU: | M02733 |
|---|---|
| Size: | 80 µl |
| Reactive Species: | Human, Mouse |
| Host: | Mouse |
| Application: | Flow Cytometry, IF, IHC-P, WB |
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Product info
Product Name
Anti-CHRM2 Antibody
SKU/Catalog Number
M02733
Size
80 µl
Description
Boster Bio Anti-CHRM2 Antibody (Catalog # M02733). Tested in IHC-P, WB, IF, Flow Cytometry application(s). This antibody reacts with Human, Mouse.
Storage & Handling
Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
Cite This Product
Anti-CHRM2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M02733)
Host
Mouse
Contents
Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Clonality
Monoclonal
Clone Number
1424CT461.78.60
Isotype
IgG1,κ
Immunogen
This antibody is generated from a mouse immunized with a recombinant protein.
Reactive Species
M02733 is reactive to CHRM2 in Human, Mouse
Calculated molecular weight
51.7 kDa
Background of CHRM2
The muscarinic acetylcholine receptor mediates various cellular responses, including inhibition of adenylate cyclase, breakdown of phosphoinositides and modulation of potassium channels through the action of G proteins. Primary transducing effect is adenylate cyclase inhibition.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M02733 is guaranteed for Flow Cytometry, IF, IHC-P, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
IF: 1:25
WB: 1:500
IHC-P: 1:25
FC: 1:25
Validation Images & Assay Conditions
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Fluorescent image of SH-SY5Y cells stained with CHRM2 Antibody. M02733 was diluted at 1:25 dilution. An Alexa Fluor® 488-conjugated goat anti-mouse lgG at 1:400 dilution was used as the secondary antibody (green). DAPI was used to stain the cell nuclear (blue).
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Western blot analysis of lysates from SH-SY5Y cell line, human brain, mouse brain tissue(from left to right), using CHRM2 Antibody. M02733 was diluted at 1:500 at each lane. A goat anti-mouse IgG H&L(HRP) at 1:3000 dilution was used as the secondary antibody. Lysates at 20μg per lane.
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Immunohistochemical analysis of paraffin-embedded H. brain section using CHRM2. M02733 was diluted at 1:25 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.
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Immunohistochemical analysis of paraffin-embedded H. heart section using CHRM2. M02733 was diluted at 1:25 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.
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Overlay histogram showing SH-SY5Y cells stained with M02733 (green line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (, 1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse lgG (166821) at 1/200 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was mouse IgG1 (1g/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.
Specific Publications For Anti-CHRM2 Antibody (M02733)
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