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SKU:PB9097
Clonality:Polyclonal
Application:IHC-P, WB
Price: $240.00
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Overview

Product Name Anti-Chromogranin A Picoband™ Antibody
Description Rabbit IgG polyclonal antibody for Chromogranin-A(CHGA) detection. Tested with WB, IHC-P in Human;Mouse;Rat.
Cite This Product Anti-Chromogranin A Picoband™ Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9097)
Replacement Item This antibody may replace the following items: sc-13090|sc-1488|sc-18222|sc-18224|sc-18232|sc-23556|sc-271738|sc-33793|sc-376813|sc-376827|sc-393941|sc-47714|sc-53013|sc-57186|sc-80795 from Santa Cruz Biotechnology.
Host Rabbit
Isotype N/A
Validated Species Human, Mouse, Rat
Application IHC-P, WB

*Our Boster Guarantee covers the use of this product in the above tested applications.

**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.

Recommended Detection Systems Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P).
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2017!
Immunogen E.coli-derived human Chromogranin A recombinant protein (Position: K321-G457). Human Chromogranin A shares 80% and 82% amino acid (aa) sequences identity with mouse and rat Chromogranin A, respectively.
Cross Reactivity No cross reactivity with other proteins
Pack Size 100μg/vial

Properties

Clonality Polyclonal
Form Lyophilized
Contents Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
*carrier free antibody available upon request.
Concentration Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Storage At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing.
Purification Immunogen affinity purified.
Isotype N/A

Protein Target Info (Source: Uniprot.org)

You can check the tissue specificity below for information on selecting positive and negative control.

Gene Name CHGA
Protein Name Chromogranin-A
Molecular Weight 50688 MW
Protein Function Pancreastatin: Strongly inhibits glucose induced insulin release from the pancreas.
Tissue Specificity GE-25 is found in the brain. .
Subcellular Localization Cytoplasmic vesicle, secretory vesicle lumen . Cytoplasmic vesicle, secretory vesicle membrane . Secreted. Associated with the secretory granule membrane through direct interaction to SCG3 that in turn binds to cholesterol-enriched lipid rafts in intragranular conditions. .
Uniprot ID P10645
Alternative Names Chromogranin-A;CgA;Pituitary secretory protein I;SP-I;Vasostatin-1;Vasostatin I;Vasostatin-2;Vasostatin II;EA-92;ES-43;Pancreastatin;SS-18;WA-8;WE-14;LF-19;Catestatin ;SL21 ;AL-11;GV-19;GR-44;ER-37;GE-25 ;Serpinin-RRG;Serpinin;p-Glu serpinin precursor;CHGA;
Research Areas |neuroscience|cell type marker|neuron marker|soma marker| tags & cell markers|cell type markers|tumor associated| signal transduction|growth factors/hormones|insulin / insulin-like| cancer|growth factors|insulin and insulin-like|tumor biomarkers|
*if product is indicated to react with multiple species, protein info is based on the human gene.

Background for Chromogranin-A

CHGA, also known as CGA or chromogranin A, is an acidic protein costored and coreleased with catecholamines from storage granules in the adrenal medulla. CHGA is a member of the granin family of neuroendocrine secretory protein, It is located in secretory vesicles of neurons and endocrine cells. In humans, CHGA protein is encoded by the CHGA gene, and it is mapped to 14q32.12. CHGA gene product is a precursor to three biologically active peptides: vasostatin, pancreastatin, and parastatin. These peptides negatively modulate the neuroendocrine function of the releasing cell (autocrine) or nearby cells (paracrine).

Anti-Chromogranin A Picoband™ Antibody Images

Click the images to enlarge.

Anti-Chromogranin A Picoband™ Antibody
Anti-Chromogranin A Picoband antibody, PB9097-1.jpg
All lanes: Anti Chromogranin A (PB9097) at 0.5ug/ml
Lane 1: Rat Liver Tissue Lysate at 50ug
Lane 2: Rat Gaster Tissue Lysate at 50ug
Lane 3: Rat Brain Tissue Lysate at 50ug
Lane 4: PC-12 Whole Cell Lysate at 40ug
Predicted bind size: 51KD
Observed bind size: 51KD
Anti-Chromogranin A Picoband™ Antibody
Anti-Chromogranin A Picoband antibody, PB9097-2.JPG
IHC(P): Mouse Brain Tissue
Anti-Chromogranin A Picoband™ Antibody
Anti-Chromogranin A Picoband antibody, PB9097-3.JPG
IHC(P): Rat Paranephros Tissue
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FAQs

Q: Do you offer BSA-free antibodies? Keyword: Bovine serum albumin, carrier protein, conjugation
A: Yes, please contact us at support@bosterbio.com for more information about BSA-free antibodies and availability. The new BSA-free formula uses trehalose as a replacement to BSA. We have tested many alternative chemicals and found that trehalose protects the antibodies the best.
Q: Can I conjugate markers to this antibody? Can I link custom conjugates to this antibody? Keyword: conjugation
A: The antibody is stored with BSA and cannot be conjugated with markers. Carrier free antibodies are available upon request. Please contact support@bosterbio.com
Q: What should I use for negative control?
A: Please contact us for negative control suggestions. You can also check expression databases such as genecards, uniprot etc. Due to logistic reasons, we do not sell serum or lysates that we use internally for positive or negative control.
Q: Where can I find troubleshooting information? What should I do if I have unexpected bands, high background, no signal, weak signal
A: You can find Boster's troubleshoot guides under tech support tab. Please contact us for further assistance on troubleshooting your experiment.
Q: What is the immunogen sequence of this antibody? Is this antibody polyclonal or monoclonal?
A: You can find the immunogen sequence under "Immunogen" and clonality in the product name.
Q: What is the expected band size? Why is it different than the observed band size?
A: The expected band size is predicted on the size of the protein. The actual band size may be affected by a few other factors including but not limited to:
1. Post-translational modification:phosphorylation, methylation, glycosylation etc. These modifications prevent SDS molecules from binding to the target protein and thus make the band size appear larger than expected
2. Post-translational cleavage: this can cause smaller bands and or multiple bands

3. Alternative splicing: the same gene can have alternative splicing patterns generating different size proteins, all with reactivities to the antibody.

4. Amino Acid R chain charge: SDS binds to positive charges. The different size and charge of the Amino Acid side chains can affect the amount of SDS binding and thus affect the observed band size.
5. Multimers: Multimers are usually broken up in reducing conditions. However if the interactions between the multimers are strong, the band may appear higher.,
Q: What is the suggested dilution ratio for Western Blot (WB), Immunohistochemistry (IHC) and or ELISA standards? What is the optimal pH for the sample?
A: Check the datasheet for the product for details on dilution ratios for different experiments. You can find the datasheet button on the right side of the product page.
Q: What is the protocol you used for your Western blotting (WB) and Immunohistochemistry (IHC)?
A: Check our protocols under the tech support tab.
Q: What are some alternative names that could be used to describe this product?
A: One other very common name is cga antibody