Anti-CIAS1/NALP3/NLRP3 Antibody Picoband™

NLRP3/NALP3 antibody

Boster Bio Anti-CIAS1/NALP3/NLRP3 Antibody Picoband™ catalog # PB9114. Tested in WB applications. This antibody reacts with Human. Cited in 3 publication(s).

Product Info Summary

SKU: PB9114
Size: 100 μg/vial
Reactive Species: Human
Host: Rabbit
Application: WB

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Product Name

Anti-CIAS1/NALP3/NLRP3 Antibody Picoband™

View all NLRP3/NALP3 Antibodies

SKU/Catalog Number

PB9114

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-CIAS1/NALP3/NLRP3 Antibody Picoband™ catalog # PB9114. Tested in WB applications. This antibody reacts with Human.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-CIAS1/NALP3/NLRP3 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9114)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E.coli-derived human CIAS1 recombinant protein (Position: D21-K194). Human CIAS1 shares 77% amino acid (aa) sequence identity with mouse CIAS1.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins

Reactive Species

PB9114 is reactive to NLRP3 in Human

Applications

PB9114 is guaranteed for WB Boster Guarantee

Observed Molecular Weight

118 kDa

Calculated molecular weight

118.173kDa

Background of NLRP3/NALP3

NLRP3 (NLR FAMILY, PYRIN DOMAIN-CONTAINING 3),also known as CIAS1, CRYOPYRIN, NALP3 or PYPAF1, is a protein that in humans is encoded by the NLRP3 gene. It is mapped to 1q44. The encoded protein may play a role in the regulation of inflammation and apoptosis. Mutation of the NALP3 nucleotide-binding domain reduced ATP binding, CASP1 activation, IL1B production, cell death, macromolecular complex formation, self-association, and association with ASC. Consistent with an essential role for Nlrp3 inflammasomes in antifungal immunity, it has been showed that Nlrp3-deficient mice are hypersusceptible to C. albicans infection. Activation of the NLRP3 inflammasome in response to virus or to RNA was dependent upon lysosomal maturation and reactive oxygen species production in human cells. The NLRP3 inflammasome senses obesity-associated danger signals and contributes to obesity-induced inflammation and insulin resistance.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml, Human

Validation Images & Assay Conditions

Gene/Protein Information For NLRP3 (Source: Uniprot.org, NCBI)

Gene Name

NLRP3

Full Name

NACHT, LRR and PYD domains-containing protein 3

Weight

118.173kDa

Superfamily

NLRP family

Alternative Names

AGTAVPRL; AII; AII/AVP; Angiotensin/vasopressin receptor AII/AVP-like; mouse NLRP3; NLRP3; AVP; C1orf7; Caterpiller protein 1.1; CIAS1; CLR1.1; Cold autoinflammatory syndrome 1 protein; Cold autoinflammatory syndrome 1; Cryopyrin; Familial cold autoinflammatory syndrome; FCAS; FCU; Leucine-rich repeat-, and PYD-containing protein 3; Muckle-Wells syndrome; MWS; NACHT; NACHT, LRR and PYD containing protein 3; NACHT, LRR and PYD domains-containing protein 3; NALP3; NLR family, pyrin domain containing 3; NLRP3 mouse; NLRP3 polyclonal; NLRP3 rat; NLRP3; Nucleotide-Binding Oligomerization Domain, Le NLRP3 AGTAVPRL, AII, AVP, C1orf7, CIAS1, CLR1.1, DFNA34, FCAS, FCAS1, FCU, KEFH, MWS, NALP3, PYPAF1 NLR family pyrin domain containing 3 NACHT, LRR and PYD domains-containing protein 3|NACHT domain-, leucine-rich repeat-, and PYD-containing protein 3|NACHT, LRR and PYD containing protein 3|PYRIN-containing APAF1-like protein 1|caterpiller protein 1.1|cold autoinflammatory syndrome 1 protein|cold-induced autoinflammatory syndrome 1 protein|cryopyrin|cryopyrin, NACHT, LRR and PYD domains - containing protein 3|deafness, autosomal dominant 34|nucleotide-binding oligomerization domain, leucine rich repeat and pyrin domain containing 3

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on NLRP3, check out the NLRP3 Infographic

NLRP3 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for NLRP3: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

Hello CJ!

PB9114 has been cited in 3 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Wei Z,Wang J,Wang Y,Wang C,Liu X,Han Z,Fu Y,Yang Z.Effects of Neutrophil Extracellular Traps on Bovine Mammary Epithelial Cells in vitro.Front Immunol.2019 May 17;10:1003.doi:10.3389/fimmu.2019.01003.PMID:31156617;PMCID:PMC6533846.
Species: Cows
PB9114 usage in article: APP:WB, SAMPLE:BMECS, DILUTION:NA

Xue Y, Wang H, Du M, Zhu Mj. J Nutr Biochem. 2014 Jul;25(7):758-64. Doi: 10.1016/J.Jnutbio.2014.03.009. Epub 2014 Apr 1. Maternal Obesity Induces Gut Inflammation And Impairs Gut Epithelial Barrier Function In Nonobese Diabetic Mice.

Wang B, Yang G, Liang X, Zhu M, Du M. Bmc Complement Altern Med. 2014 May 20;14:162. Doi: 10.1186/1472-6882-14-162. Grape Seed Extract Prevents Skeletal Muscle Wasting In Interleukin 10 Knockout Mice.

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14 Customer Q&As for Anti-CIAS1/NALP3/NLRP3 Antibody Picoband™

Question

Is this PB9114 anti-CIAS1/NALP3/NLRP3 antibody reactive to the isotypes of NLRP3?

Verified Customer

Verified customer

Asked: 2020-03-05

Answer

The immunogen of PB9114 anti-CIAS1/NALP3/NLRP3 antibody is E.coli-derived human CIAS1 recombinant protein (Position: D21-K194). Human CIAS1 shares 77% amino acid (aa) sequence identity with mouse CIAS1. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2020-03-05

Question

My colleagues were satisfied with the WB result of your anti-CIAS1/NALP3/NLRP3 antibody. However we have been able to see positive staining in umbilical cord blood cytosol using this antibody. Is that expected? Could you tell me where is NLRP3 supposed to be expressed?

Verified Customer

Verified customer

Asked: 2020-02-28

Answer

From what I have seen in literature, umbilical cord blood does express NLRP3. Generally NLRP3 expresses in cytoplasm, cytosol, golgi apparatus membrane. note=(microbial. Regarding which tissues have NLRP3 expression, here are a few articles citing expression in various tissues:
Brain, Pubmed ID: 14702039, 16710414
Colon, Pubmed ID: 15489334
Umbilical cord blood, Pubmed ID: 11042152

Boster Scientific Support

Answered: 2020-02-28

Question

I would like using your anti-CIAS1/NALP3/NLRP3 antibody for signal transduction studies. Has this antibody been tested with western blotting on hela whole cell lysate? We would like to see some validation images before ordering.

Verified Customer

Verified customer

Asked: 2020-02-27

Answer

Thank you for your inquiry. This PB9114 anti-CIAS1/NALP3/NLRP3 antibody is validated on hela whole cell lysate, jurkat whole cell lysate, hepg2 whole cell lysate. It is guaranteed to work for WB in human. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.

Boster Scientific Support

Answered: 2020-02-27

Question

I would like to test anti-CIAS1/NALP3/NLRP3 antibody PB9114 on human umbilical cord blood for research purposes, then I may be interested in using anti-CIAS1/NALP3/NLRP3 antibody PB9114 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

Verified Customer

Verified customer

Asked: 2020-01-20

Answer

The products we sell, including anti-CIAS1/NALP3/NLRP3 antibody PB9114, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2020-01-20

Question

We have been able to see staining in human colon. Are there any suggestions? Is anti-CIAS1/NALP3/NLRP3 antibody supposed to stain colon positively?

Verified Customer

Verified customer

Asked: 2019-10-17

Answer

Based on literature colon does express NLRP3. Based on Uniprot.org, NLRP3 is expressed in blood, brain, colon, umbilical cord blood, among other tissues. Regarding which tissues have NLRP3 expression, here are a few articles citing expression in various tissues:
Brain, Pubmed ID: 14702039, 16710414
Colon, Pubmed ID: 15489334
Umbilical cord blood, Pubmed ID: 11042152

Boster Scientific Support

Answered: 2019-10-17

Question

Does PB9114 anti-CIAS1/NALP3/NLRP3 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

Verified Customer

Verified customer

Asked: 2019-08-02

Answer

You can see on the product datasheet, PB9114 anti-CIAS1/NALP3/NLRP3 antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2019-08-02

Question

We are currently using anti-CIAS1/NALP3/NLRP3 antibody PB9114 for human tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human. Is it possible that the antibody can work on zebrafish tissues as well?

Verified Customer

Verified customer

Asked: 2019-07-30

Answer

The anti-CIAS1/NALP3/NLRP3 antibody (PB9114) has not been tested for cross reactivity specifically with zebrafish tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in zebrafish you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2019-07-30

Question

We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for umbilical cord blood using anti-CIAS1/NALP3/NLRP3 antibody PB9114. Let me know if you need anything else.

Verified Customer

Verified customer

Asked: 2019-05-21

Answer

We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2019-05-21

Question

My question regarding product PB9114, anti-CIAS1/NALP3/NLRP3 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

Verified Customer

Verified customer

Asked: 2018-06-28

Answer

We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free PB9114 anti-CIAS1/NALP3/NLRP3 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2018-06-28

Question

I see that the anti-CIAS1/NALP3/NLRP3 antibody PB9114 works with WB, what is the protocol used to produce the result images on the product page?

C. Baker

Verified customer

Asked: 2016-10-20

Answer

You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2016-10-20

Question

Is a blocking peptide available for product anti-CIAS1/NALP3/NLRP3 antibody (PB9114)?

S. Miller

Verified customer

Asked: 2016-05-02

Answer

We do provide the blocking peptide for product anti-CIAS1/NALP3/NLRP3 antibody (PB9114). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2016-05-02

Question

Would anti-CIAS1/NALP3/NLRP3 antibody PB9114 work for WB with umbilical cord blood?

A. Baker

Verified customer

Asked: 2015-11-30

Answer

According to the expression profile of umbilical cord blood, NLRP3 is highly expressed in umbilical cord blood. So, it is likely that anti-CIAS1/NALP3/NLRP3 antibody PB9114 will work for WB with umbilical cord blood.

Boster Scientific Support

Answered: 2015-11-30

Question

I have attached the WB image, lot number and protocol we used for umbilical cord blood using anti-CIAS1/NALP3/NLRP3 antibody PB9114. Please let me know if you require anything else.

C. Banerjee

Verified customer

Asked: 2013-11-04

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2013-11-04

Question

Do you have a BSA free version of anti-CIAS1/NALP3/NLRP3 antibody PB9114 available?

A. Kulkarni

Verified customer

Asked: 2013-01-31

Answer

Thank you for your recent telephone inquiry. I can confirm that some lots of this anti-CIAS1/NALP3/NLRP3 antibody PB9114 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2013-01-31

Order DetailsPrice
PB9114

100μg

$370
PB9114-10ug

10μg sample (liquid)

$99
PB9114-Biotin

100 μg Biotin conjugated

$570
PB9114-Cy3

100 μg Cy3 conjugated

$570
PB9114-Dylight488

100 μg Dylight488 conjugated

$570
PB9114-Dylight550

100 μg Dylight550 conjugated

$570
PB9114-Dylight594

100 μg Dylight594 conjugated

$570
PB9114-FITC

100 μg FITC conjugated

$570
PB9114-HRP

100 μg HRP conjugated

$570
PB9114-APC

100 μg APC conjugated

$670
PB9114-PE

100 μg PE conjugated

$670
PB9114-carrier-free

Carrier Free

$370

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