Anti-Cell death activator CIDE-B CIDEB Antibody

Cideb antibody

Boster Bio Anti-Cell death activator CIDE-B CIDEB Antibody (Catalog # A07404). Tested in WB, IHC, IF applications. This antibody reacts with Human, Mouse.

Product Info Summary

SKU: A07404
Size: 100 µl
Reactive Species: Human, Mouse
Host: Rabbit
Application: IF, IHC, WB

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Product Name

Anti-Cell death activator CIDE-B CIDEB Antibody

View all Cideb Antibodies

SKU/Catalog Number

A07404

Size

100 µl

Form

Liquid

Description

Boster Bio Anti-Cell death activator CIDE-B CIDEB Antibody (Catalog # A07404). Tested in WB, IHC, IF applications. This antibody reacts with Human, Mouse.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Cell death activator CIDE-B CIDEB Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A07404)

Host

Rabbit

Contents

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Clonality

Polyclonal

Isotype

IgG

Immunogen

Synthesized peptide derived from internal of human CIDEB.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

A07404 is reactive to Cideb in Human, Mouse

Applications

A07404 is guaranteed for IF, IHC, WB Boster Guarantee

Observed Molecular Weight

Calculated molecular weight

24.8kDa

Background of Cideb

Activates apoptosis.

Lugovskoy A.A., Cell 99:747-755(1999).
Inohara N., EMBO J. 17:2526-2533(1998).
Ota T., Nat. Genet. 36:40-45(2004).

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blotting: 1:500~1:3000
Immunohistochemistry: 1:50~1:100
Immunofluorescence: 1:100~1:500

Validation Images & Assay Conditions

Gene/Protein Information For Cideb (Source: Uniprot.org, NCBI)

Gene Name

Cideb

Full Name

Cell death activator CIDE-B

Weight

24.8kDa

Alternative Names

cell death-inducing DFFA-like effector bcell death activator CIDE-B Cideb|1110030C18Rik, AI790179, CIDE-, CIDE-B|cell death-inducing DNA fragmentation factor, alpha subunit-like effector B|cell death activator CIDE-B|DFFA-like B|cell death-inducing DFFA-like effector B

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on Cideb, check out the Cideb Infographic

Cideb infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for Cideb: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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7 Customer Q&As for Anti-Cell death activator CIDE-B CIDEB Antibody

Question

We are currently using anti-CIDEB antibody A07404 for human tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human, mouse. Is it likely that the antibody can work on feline tissues as well?

B. Wu

Verified customer

Asked: 2019-12-23

Answer

The anti-CIDEB antibody (A07404) has not been tested for cross reactivity specifically with feline tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in feline you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2019-12-23

Question

See attached the WB image, lot number and protocol we used for ileal mucosa using anti-CIDEB antibody A07404. Please let me know if you require anything else.

Verified Customer

Verified customer

Asked: 2019-11-28

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2019-11-28

Question

My question regarding product A07404, anti-CIDEB antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

Verified Customer

Verified customer

Asked: 2019-11-19

Answer

We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A07404 anti-CIDEB antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2019-11-19

Question

Does anti-CIDEB antibody A07404 work on bovine WB with liver?

Verified Customer

Verified customer

Asked: 2019-08-12

Answer

Our lab technicians have not validated anti-CIDEB antibody A07404 on bovine. You can run a BLAST between bovine and the immunogen sequence of anti-CIDEB antibody A07404 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated bovine samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in bovine liver in WB, you can get your next antibody for free.

Boster Scientific Support

Answered: 2019-08-12

Question

We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for ileal mucosa using anti-CIDEB antibody A07404. Let me know if you need anything else.

P. Banerjee

Verified customer

Asked: 2018-07-02

Answer

Thanks for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2018-07-02

Question

Is a blocking peptide available for product anti-CIDEB antibody (A07404)?

E. Zhao

Verified customer

Asked: 2014-04-18

Answer

We do provide the blocking peptide for product anti-CIDEB antibody (A07404). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2014-04-18

Question

Would A07404 anti-CIDEB antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

F. Johnson

Verified customer

Asked: 2013-12-11

Answer

As indicated on the product datasheet, A07404 anti-CIDEB antibody as been tested on IHC. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2013-12-11

Order DetailsPrice
A07404-100uL

100uL

$360

Free Secondary Antibody

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A07404
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*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$360.00

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