Product Info Summary
SKU: | A00590-1 |
---|---|
Size: | 100ul |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | IF, IHC, WB |
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Product info
Product Name
Anti-Clusterin-Alpha (E436) Antibody
View all Clusterin/APOJ Antibodies
SKU/Catalog Number
A00590-1
Size
100ul
Form
Liquid
Description
Boster Bio Anti-Clusterin-Alpha (E436) Antibody catalog # A00590-1. Tested in WB,IHC,IF applications. This antibody reacts with Human,Mouse,Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Clusterin-Alpha (E436) Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00590-1)
Host
Rabbit
Contents
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from human Integrin beta3 around the phosphorylation site of Y785.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A00590-1 is reactive to CLU in Human, Mouse, Rat
Applications
A00590-1 is guaranteed for IF, IHC, WB Boster Guarantee
Observed Molecular Weight
Calculated molecular weight
52.495kDa
Background of Clusterin/APOJ
Transcriptional regulator that binds to DNA as a dimer or as a tetramer, but not as a monomer. Binds to G-doublets in an A/T-rich environment; the preferred motif is a tandem repeat of 5'-. ATTGGTTA-3' combined with a 5'-TTATTA-3' box. Binds to nucleosomes By similarity. Binds to chromatin and interacts selectively with histone H3 that is not methylated at 'Lys-4', not phosphorylated at 'Thr-3' and not methylated at 'Arg-2'. Functions as a sensor of histone H3 modifications that are important for the epigenetic regulation of gene expression. Functions as a transcriptional activator and promotes the expression of otherwise tissue-specific self-antigens in the thymus, which is important for self tolerance and the avoidance of autoimmune reactions.
Nagamine K., Nat. Genet. 17:393-398(1997).
Aaltonen J., Nat. Genet. 17:399-403(1997).
Hattori M., Nature 405:311-319(2000).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:500-1:1000
IHC: 1:50-1:200
IF: 1:50-1:200
Validation Images & Assay Conditions
Click image to see more details
Figure 2. Immunohistochemistry validation of CLU using Anti-Clusterin-Alpha (E436) Antibody (A00590-1).
Immunohistochemistry (IHC) analyzes of Clusterin-α (E436) pAb in paraffin-embedded human tonsil carcinoma tissue at 1:50.showing cytoplasmic and nucleus /cell membrane staining. Negative control (the right)Using PBS instead of primary antibodyClick image to see more details
Figure 1. Western blotting validation for Anti-Clusterin-Alpha (E436) Antibody A00590-1
Western blot (WB) analysis of Clusterin-α (E436) pAb at 1:500 dilutionLane1:Hela whole cell lysate(40ug)
Lane2:A549 whole cell lysate(40ug)
Lane3:SGC7901 whole cell lysate(40ug)
Electrophoresis was performed on a SDS-PAGE gel. To determine SDS-PAGE gel concentration
Protein Target Info & Infographic
Gene/Protein Information For CLU (Source: Uniprot.org, NCBI)
Gene Name
CLU
Full Name
Clusterin
Weight
52.495kDa
Superfamily
clusterin family
Alternative Names
40; 40, sulfated glycoprotein 2; Aging-associated gene 4 protein; aging-associated protein 4; APOJ; apo-J; Apolipoprotein J; CLI; CLIclusterin (complement lysis inhibitor, SP-40; CLU; Clusterin; Complement cytolysis inhibitor; complement lysis inhibitor; Complement-associated protein SP-40; Ku70-binding protein 1; KUB1SGP2; MGC24903; NA1/NA2; SGP-2; SP-40; sulfated glycoprotein 2; Testosterone-repressed prostate message 2; testosterone-repressed prostate message 2, apolipoprotein J); TRPM-2; TRPM-2TRPM2 CLU AAG4, APO-J, APOJ, CLI1, CLU2, KUB1, NA1/NA2, SGP-2, SGP2, SP-40, TRPM-2, TRPM2, CLU clusterin clusterin|aging-associated protein 4|apolipoprotein J|complement cytolysis inhibitor|complement lysis inhibitor|complement-associated protein SP-40,40|epididymis secretory sperm binding protein|ku70-binding protein 1|sulfated glycoprotein 2|testosterone-repressed prostate message 2
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CLU, check out the CLU Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CLU: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Clusterin-Alpha (E436) Antibody (A00590-1)
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No publications found for A00590-1
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