Data & Images
|Product Name||Anti-CMA1/Chymase Picoband™ Antibody|
|Description||Rabbit IgG polyclonal antibody for Chymase(CMA1) detection. Tested with WB, IHC-P in Human.|
|Cite This Product||Anti-CMA1/Chymase Picoband™ Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9055)|
|Replacement Item||This antibody may replace the following items: sc-324924|sc-324926|sc-56425|sc-59586|sc-71540 from Santa Cruz Biotechnology.|
*Our Boster Guarantee covers the use of this product in the above tested applications.
**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.
|Recommended Detection Systems||Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P).
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2017!
|Immunogen||E.coli-derived human CMA1 recombinant protein (Position: I22-N247). Human CMA1 shares 75% and 74% amino acid (aa) sequences identity with mouse and rat CMA1, respectively.|
|Cross Reactivity||No cross reactivity with other proteins|
|Contents||Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
*carrier free antibody available upon request.
|Concentration||Add 0.2ml of distilled water will yield a concentration of 500ug/ml.|
|Storage||At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing.|
|Purification||Immunogen affinity purified.|
Protein Target Info (Source: Uniprot.org)
You can check the tissue specificity below for information on selecting positive and negative control.
|Molecular Weight||27325 MW|
|Protein Function||Major secreted protease of mast cells with suspected roles in vasoactive peptide generation, extracellular matrix degradation, and regulation of gland secretion.|
|Tissue Specificity||Mast cells in lung, heart, skin and placenta. Expressed in both normal skin and in urticaria pigmentosa lesions. .|
|Sequence Similarities||Belongs to the peptidase S1 family. Granzyme subfamily.|
|Subcellular Localization||Secreted. Cytoplasmic granule. Mast cell granules.|
|Alternative Names||Chymase;184.108.40.206;Alpha-chymase;Mast cell protease I;CMA1;CYH, CYM;|
|Research Areas|||immunology|innate immunity|macrophage / inflamm.| cardiovascular|blood| signal transduction|cytoskeleton / ecm|extracellular matrix|ecm enzymes|other enzymes|atherosclerosis|vascular inflammation|inflammatory mediators||
Background for Chymase
Dilution Ratios/Recommended Concentrations
At Boster we strive to provide the best Anti-CMA1/Chymase Picoband™ Antibody by testing all applications on non-spiked tissues and cell lines to ensure that the affinity of the antibody is enough to react to the endogenouse level of the target protein. Read more about our QC panel here.
|Recommended dilution ratios are listed below:|
Immunohistochemistry(Paraffin-embedded Section), 0.5-1μg/ml, Human, By Heat|
Western blot, 0.1-0.5μg/ml, Human
**Boster provides high sensitivity secondary antibody kits for Western blotting and IHC. For more info see Related Products below.
Anti-CMA1/Chymase Picoband™ Antibody Images
Click the images to enlarge.
IHC(P): Human Placenta Tissue
All lanes: Anti-CMA1(PB9055) at 0.5ug/ml
WB: Recombinant Human CMA1 Protein 0.5ng
Predicted bind size: 47KD
Observed bind size: 47KD
1. Post-translational modification:phosphorylation, methylation, glycosylation etc. These modifications prevent SDS molecules from binding to the target protein and thus make the band size appear larger than expected
2. Post-translational cleavage: this can cause smaller bands and or multiple bands
3. Alternative splicing: the same gene can have alternative splicing patterns generating different size proteins, all with reactivities to the antibody.
4. Amino Acid R chain charge: SDS binds to positive charges. The different size and charge of the Amino Acid side chains can affect the amount of SDS binding and thus affect the observed band size.
5. Multimers: Multimers are usually broken up in reducing conditions. However if the interactions between the multimers are strong, the band may appear higher.,