|Application:||ELISA, IHC-P, WB|
Data & Images
|Product Name||Anti-CNTF Picoband™ Antibody|
|Description||Rabbit IgG polyclonal antibody for Ciliary neurotrophic factor(CNTF) detection. Tested with WB, IHC-P, ELISA in Mouse;Rat.|
|Cite This Product||Anti-CNTF Picoband™ Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9067)|
|Replacement Item||This antibody may replace the following items: sc-13996|sc-1912|sc-365211|sc-166272|sc-365210|sc-1911|sc-166273|sc-25286|sc-1912-R from Santa Cruz Biotechnology.|
|Validated Species||Mouse, Rat|
|Application||ELISA, IHC-P, WB
*Our Boster Guarantee covers the use of this product in the above tested applications.
**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.
|Recommended Detection Systems||Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P).
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2017!
|Immunogen||E. coli-derived rat CNTF recombinant protein (Position: A2-M200). Rat CNTF shares 84% and 95% amino acid (aa) sequences identity with human and mouse CNTF, respectively.|
|Cross Reactivity||No cross reactivity with other proteins|
|Contents||Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
*carrier free antibody available upon request.
|Concentration||Add 0.2ml of distilled water will yield a concentration of 500ug/ml.|
|Storage||At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing.|
|Purification||Immunogen affinity purified.|
Protein Target Info (Source: Uniprot.org)
You can check the tissue specificity below for information on selecting positive and negative control.
|Protein Name||Ciliary neurotrophic factor|
|Molecular Weight||22854 MW|
|Protein Function||CNTF is a survival factor for various neuronal cell types. Seems to prevent the degeneration of motor axons after axotomy.|
|Tissue Specificity||Nervous system.|
|Alternative Names||Ciliary neurotrophic factor;CNTF;Cntf;|
|Research Areas|||neuroscience|neurology process|growth and development|neurotrophins| neuroscience|cell type marker|neural stem cell marker| stem cells|neural stem cells|glial restricted lineage|glial progenitors||
Background for Ciliary neurotrophic factor
Dilution Ratios/Recommended Concentrations
At Boster we strive to provide the best Anti-CNTF Picoband™ Antibody by testing all applications on non-spiked tissues and cell lines to ensure that the affinity of the antibody is enough to react to the endogenouse level of the target protein. Read more about our QC panel here.
|Recommended dilution ratios are listed below:|
ELISA , 0.1-0.5μg/ml, Rat, -|
Immunohistochemistry(Paraffin-embedded Section), 0.5-1μg/ml, Mouse, Rat, By Heat
Western blot, 0.1-0.5μg/ml, Rat
**Boster provides high sensitivity secondary antibody kits for Western blotting and IHC. For more info see Related Products below.
Anti-CNTF Picoband™ Antibody Images
Click the images to enlarge.
IHC(P): Mouse Brain Tissue
IHC(P): Rat Brain Tissue
All lanes: Anti-CNTF(PB9067) at 0.5ug/ml
Lane 1: PC-12 Whole Cell Lysate at 40ug
Lane 2: NRK Whole Cell Lysate at 40ug
Lane 3: RH35 Whole Cell Lysate at 40ug
Lane 4: Rat Brain Tissue Lysate at 40ug
Lane 5: Rat Liver Tissue Lysate at 40ug
Lane 6: Rat Spleen Tissue Lysate at 40ug
Lane 7: Rat Testis Tissue Lysate at 40ug
Predicted bind size: 23KD
Observed bind size: 23KD
1. Post-translational modification:phosphorylation, methylation, glycosylation etc. These modifications prevent SDS molecules from binding to the target protein and thus make the band size appear larger than expected
2. Post-translational cleavage: this can cause smaller bands and or multiple bands
3. Alternative splicing: the same gene can have alternative splicing patterns generating different size proteins, all with reactivities to the antibody.
4. Amino Acid R chain charge: SDS binds to positive charges. The different size and charge of the Amino Acid side chains can affect the amount of SDS binding and thus affect the observed band size.
5. Multimers: Multimers are usually broken up in reducing conditions. However if the interactions between the multimers are strong, the band may appear higher.,