Anti-Collagen alpha-1(XIV) chain COL14A1 Antibody

COL14A1 antibody

Boster Bio Anti-Collagen alpha-1 (XIV) chain COL14A1 Antibody catalog # A06685. Tested in IHC applications. This antibody reacts with Human, Mouse, Rat.

Product Info Summary

SKU: A06685
Size: 100μl
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: IHC

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Product Name

Anti-Collagen alpha-1(XIV) chain COL14A1 Antibody

View all COL14A1 Antibodies

SKU/Catalog Number

A06685

Size

100μl

Form

Liquid

Description

Boster Bio Anti-Collagen alpha-1 (XIV) chain COL14A1 Antibody catalog # A06685. Tested in IHC applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Collagen alpha-1(XIV) chain COL14A1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A06685)

Host

Rabbit

Contents

PBS, pH 7.4, containing 0.02% sodium azide as Preservative and 50% Glycerol.

Clonality

Polyclonal

Clone Number

2B4

Isotype

IgG

Immunogen

Synthetic peptide from human protein at AA range: 1571-1620.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

A06685 is reactive to COL14A1 in Human, Mouse, Rat

Applications

A06685 is guaranteed for IHC Boster Guarantee

Observed Molecular Weight

39 kDa

Calculated molecular weight

193.515kDa

Background of COL14A1

Mediator of RNA polymerase II transcription subunit 4, also known as mediator complex subunit 4 (MED4), a component of Mediator or vitamin D3 receptor-interacting protein complex 36 kDa component (DRIP36), is a protein that in humans is encoded by the MED4 gene. The Mediator complex interacts with DNA-binding gene-specific transcription factors to modulate transcription by RNA polymerase II. The MED4 gene was mapped to chromosome 13 based on sequence similarity between the MED4 sequence and a genomic contig.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

IHC-P, 1:50-300

Validation Images & Assay Conditions

Gene/Protein Information For COL14A1 (Source: Uniprot.org, NCBI)

Gene Name

COL14A1

Full Name

Collagen alpha-1(XIV) chain

Weight

193.515kDa

Superfamily

fibril-associated collagens with interrupted helices (FACIT) family

Alternative Names

collagen alpha-1(XIV) chain; collagen, type XIV, alpha 1; undulin (fibronectin-tenascin-related); Undulin; UNDundulin COL14A1 UND collagen type XIV alpha 1 chain collagen alpha-1(XIV) chain|collagen, type XIV, alpha 1|undulin (fibronectin-tenascin-related)

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on COL14A1, check out the COL14A1 Infographic

COL14A1 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for COL14A1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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5 Customer Q&As for Anti-Collagen alpha-1(XIV) chain COL14A1 Antibody

Question

Would A06685 anti-COL14A1 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

Verified Customer

Verified customer

Asked: 2020-03-27

Answer

As indicated on the product datasheet, A06685 anti-COL14A1 antibody as been validated on IHC. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2020-03-27

Question

We are currently using anti-COL14A1 antibody A06685 for rat tissue, and we are content with the IHC results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on zebrafish tissues as well?

Verified Customer

Verified customer

Asked: 2019-11-28

Answer

The anti-COL14A1 antibody (A06685) has not been validated for cross reactivity specifically with zebrafish tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in zebrafish you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2019-11-28

Question

Is this A06685 anti-COL14A1 antibody reactive to the isotypes of COL14A1?

Verified Customer

Verified customer

Asked: 2018-12-11

Answer

The immunogen of A06685 anti-COL14A1 antibody is Synthetic peptide from human protein at AA range: 1571-1620. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2018-12-11

Question

I have attached the WB image, lot number and protocol we used for descending thoracic aorta using anti-COL14A1 antibody A06685. Please let me know if you require anything else.

J. Patel

Verified customer

Asked: 2017-05-29

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2017-05-29

Question

Thank you for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for descending thoracic aorta using anti-COL14A1 antibody A06685. Let me know if you need anything else.

S. Walker

Verified customer

Asked: 2015-03-06

Answer

Thank you for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2015-03-06

Order DetailsPrice
A06685

100uL

$399

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