Show Order Info
SKU:PA2140-2
Pack Size:100μg/vial
Sample Size:30ug for $99, contact us for details
Clonality:Polyclonal
Application:IHC-P, IHC-F, ICC, WB
Price: $200.00
Quantity:
Technical
Specs
Kit
Components
Gene
Information
Application
Data & Images
Reviews
Publications
FAQs

Overview

Product Name Anti-Collagen I Antibody
SKU/Catalog Number PA2140-2
Description Rabbit IgG polyclonal antibody for Collagen alpha-1(I) chain(COL1A1) detection. Tested with WB, IHC-P, IHC-F, ICC in Human;Mouse;Rat.
Cite This Product Anti-Collagen I Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA2140-2)
Replacement Item This antibody may replace the following items: sc-25974|sc-8783|sc-28657|sc-8784|sc-8784-R from Santa Cruz Biotechnology.
Host Rabbit
Isotype N/A
Validated Species Human, Mouse, Rat
Predicted Species Hamster

*This antibody is predicted to react with the above species based on antigen sequence similarities. Our Boster Guarantee covers the use of this product with the above species.

Application IHC-P, IHC-F, ICC, WB

*Our Boster Guarantee covers the use of this product in the above tested applications.

**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.

Recommended Detection Systems Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P), IHC(F) and ICC.
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2017!
Immunogen A synthetic peptide corresponding to a sequence at the C-terminus of mouse Collagen I(1192-1207aa QPPQEKSQDGGRYYRA), identical to the related rat sequence, and different from the related human sequence by two amino acids.
Cross Reactivity No cross reactivity with other proteins
Pack Size 100μg/vial

Properties

Clonality Polyclonal
Form Lyophilized
Contents Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
*carrier free antibody available upon request.
Concentration Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Storage At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing.
Purification Immunogen affinity purified.
Isotype N/A

Protein Target Info (Source: Uniprot.org)

You can check the tissue specificity below for information on selecting positive and negative control.

Gene Name Col1a1
Protein Name Collagen alpha-1(I) chain
Molecular Weight 138032 MW
Protein Function Type I collagen is a member of group I collagen (fibrillar forming collagen).
Tissue Specificity Forms the fibrils of tendon, ligaments and bones. In bones the fibrils are mineralized with calcium hydroxyapatite.
Sequence Similarities Belongs to the fibrillar collagen family.
Subcellular Localization Secreted, extracellular space, extracellular matrix .
Uniprot ID P11087
Alternative Names Collagen alpha-1(I) chain;Alpha-1 type I collagen;Col1a1;Cola1;
Research Areas |signal transduction|cytoskeleton / ecm|extracellular matrix|ecm proteins|collagen| stem cells|mesenchymal stem cells|osteogenesis|
*if product is indicated to react with multiple species, protein info is based on the human gene.

Background for Collagen alpha-1(I) chain

Collagen, type I, alpha 1, also known as COL1A1, is a human gene that encodes the major component of type I collagen, the fibrillar collagen found in most connective tissues, including cartilage. This gene is mapped to 17q21.33. This gene encodes the pro-alpha1 chains of type I collagen whose triple helix comprises two alpha1 chains and one alpha2 chain. Type I is a fibril-forming collagen found in most connective tissues and is abundant in bone, cornea, dermis and tendon. Mutations in this gene are associated with osteogenesis imperfecta types I-IV, Ehlers-Danlos syndrome type VIIA, Ehlers-Danlos syndrome Classical type, Caffey Disease and idiopathic osteoporosis.

Anti-Collagen I Antibody Images

Click the images to enlarge.

Anti-Collagen I Antibody
Anti-Collagen I antibody, PA2140-2, IHC(P)
IHC(P): Rat Lung Tissue
Anti-Collagen I Antibody
Anti-Collagen I antibody, PA2140-2, IHC(P)
IHC(P): Human Intestinal Cancer Tissue
Anti-Collagen I Antibody
Anti-Collagen I antibody, PA2140-2, ICC
ICC: NIH3T3 Cell
Anti-Collagen I Antibody
Anti-Collagen I antibody, PA2140-2, IHC(F)
IHC(F): Human Placenta Tissue
Anti-Collagen I Antibody
Anti-Collagen I antibody, PA2140-2, Western blotting
Lane 1: Rat Lung Tissue Lysate
Lane 2: Human Placenta Tissue Lysate
Lane 3: Rat Testis Tissue Lysate
Write a review for PA2140-2

Publications

Ge Ws, Wang Yj, Wu Jx, Fan Jg, Chen Yw, Zhu L. Mol Med Rep. 2014 Jun;9(6):2145-51. Doi: 10.3892/Mmr.2014.2099. Epub 2014 Apr 1. ??-Catenin Is Overexpressed In Hepatic Fibrosis And Blockage Of Wnt/??-Catenin Signaling Inhibits Hepatic Stellate Cell A...
Li X, Du J, Jin H, Geng B, Tang C. Heart Vessels. 2008 Nov;23(6):409-19. Doi: 10.1007/S00380-008-1059-4. Epub 2008 Nov 27. Sodium Hydrosulfide Alleviates Pulmonary Artery Collagen Remodeling In Rats With High Pulmonary Blood Flow.
Zhao Cy, Chen Jt, Yang Dh, Zhong Zm, Bai L. Osteoporos Int. 2010 Jan;21(1):129-35. Doi: 10.1007/S00198-009-0928-8. Epub 2009 Apr 15. Effects Of Extracts Of Oxyntic Mucosa In Rat On The Biological Activity Of Osteoblasts.
Zhang Lf, Ding Wh, Shi Lb, Li K, Haom Yj, Ke Yn, Tang Zs. Clin Exp Pharmacol Physiol. 2010 Apr;37(4):477-81. Doi: 10.1111/J.1440-1681.2009.05336.X. Epub 2009 Nov 23. Effects Of Exogenous Urotensin Ii On Vascular Remodelling After Balloon Injury.
Fu Z, Liu P, Yang D, Wang F, Yuan L, Lin Z, Jiang J. Int J Mol Med. 2012 Oct;30(4):805-10. Doi: 10.3892/Ijmm.2012.1073. Epub 2012 Jul 20. Interleukin-18-Induced Inflammatory Responses In Synoviocytes And Chondrocytes From Osteoarthriti...
Liu L, Liu Q, Lin X, Wei Qj, Zheng L. In Vitro Cell Dev Biol Anim. 2014 Dec;50(10):982-91. Doi: 10.1007/S11626-014-9795-5. Epub 2014 Aug 15. Effect Of Jeztc, A Synthetic Compound, On Proliferation And Phenotype Maintenance Of Rabbit Articular Chon...
Liu Q, Lu Z, Wu H, Zheng L. Tohoku J Exp Med. 2015;235(3):201-13. Doi: 10.1620/Tjem.235.201. Chondroprotective Effects Of Taurine In Primary Cultures Of Human Articular Chondrocytes.
Li J, Yang B, Qian Y, Wang Q, Han R, Hao T, Shu Y,, Zhang Y, Yao F, Wang C. J Biomed Mater Res B Appl Biomater. 2015 Oct;103(7):1498-510. Doi: 10.1002/Jbm.B.33339. Epub 2014 Nov 29. Iota-Carrageenan/Chitosan/Gelatin Scaffold For The Osteogenic Dif...
Zhang K, Wang L, Zhang S, Yu B, Liu F, Cui Z, Jin D, Bai X. Eur J Orthop Surg Traumatol. 2013 Feb;23(2):145-8. Doi: 10.1007/S00590-012-0944-9. Epub 2012 Feb 10. Celecoxib Inhibits The Heterotopic Ossification In The Rat Model With Achilles Tenotomy.
Zhang Y, Yang Jh. J Cell Biochem. 2013 Nov;114(11):2595-602. Doi: 10.1002/Jcb.24607. Activation Of The Pi3K/Akt Pathway By Oxidative Stress Mediates High Glucose-Induced Increase Of Adipogenic Differentiation In Primary Rat Osteoblasts.
Ma X, Xu L, Wang S, Chen H, Xu J, Li X, Ning G. Lab Invest. 2009 Aug;89(8):903-14. Doi: 10.1038/Labinvest.2009.51. Epub 2009 Jun 1. Loss Of Steroid Receptor Co-Activator-3 Attenuates Carbon Tetrachloride-Induced Murine Hepatic Injury And Fibrosis.
Wang W, Zhang X, Zheng J, Yang J. Mol Cell Biochem. 2010 May;338(1-2):115-22. Doi: 10.1007/S11010-009-0344-6. Epub 2009 Dec 1. High Glucose Stimulates Adipogenic And Inhibits Osteogenic Differentiation In Mg-63 Cells Through Camp/Protein Kinase A/...
Liu X, Li P, Chen Xy, Zhou Yg. Clin Exp Dermatol. 2010 Jun;35(4):417-24. Doi: 10.1111/J.1365-2230.2009.03606.X. Epub 2009 Oct 23. C-Ski Promotes Skin Fibroblast Proliferation But Decreases Type I Collagen: Implications For Wound Healing And Scar F...
Xia C, Yang X, Wang Yz, Sun K, Ji L, Tian S. Orthopedics. 2010 Nov 2;33(11):809. Doi: 10.3928/01477447-20100924-06. Tendon Healing In Vivo And In Vitro: Neutralizing Antibody To Tgf-?? Improves Range Of Motion After Flexor Tendon Repair.
Zhao L, Zhao J, Wang S, Wang J, Liu J. J Biomed Mater Res B Appl Biomater. 2011 Apr;97(1):1-9. Doi: 10.1002/Jbm.B.31768. Epub 2011 Feb 2. Comparative Study Between Tissue-Engineered Periosteum And Structural Allograft In Rabbit Critical-Sized Radi...
Wang Z, Xu J, Zheng Y, Chen W, Sun Y, Wu Z, Luo M. Hepatol Res. 2011 May;41(5):475-83. Doi: 10.1111/J.1872-034X.2011.00794.X. Effect Of The Regulation Of Retinoid X Receptor-?? Gene Expression On Rat Hepatic Fibrosis.
Long D, Lu J, Luo L, Guo Y, Li C, Wu W, Shan J, Li L, Li S, Li Y, Lin T, Feng L. J Surg Res. 2012 Jul;176(1):248-59. Doi: 10.1016/J.Jss.2011.06.009. Epub 2011 Jul 5. Effects Of Octreotide On Activated Pancreatic Stellate Cell-Induced Pancreas Graf...
Zhang Y, Li S, Wu C. J Biomed Mater Res A. 2014 Jan;102(1):105-16. Doi: 10.1002/Jbm.A.34679. Epub 2013 Apr 18. The In Vitro And In Vivo Cementogenesis Of Camgsi???O
Ye T, Wang Q, Zhang Y, Song X, Yang D, Li D, Li D, Su L, Yang Y, Ma S. Plos One. 2015 Mar 18;10(3):E0120178. Doi: 10.1371/Journal.Pone.0120178. Ecollection 2015. Over-Expression Of Calpastatin Inhibits Calpain Activation And Attenuates Post-Infarc...
Jiang Gt, Chen X, Li D, An Hx, Jiao Jd. Mol Med Rep. 2014 Sep;10(3):1501-8. Doi: 10.3892/Mmr.2014.2323. Epub 2014 Jun 13. Ulinastatin Attenuates Renal Interstitial Inflammation And Inhibits Fibrosis Progression In Rats Under Unilateral Ureteral Ob...
Yang L, Ni L, Duan Q, Wang X, Chen C, Chen S, Chaugai S, Zeldin Dc, Tang Jr, Wang Dw. Prostaglandins Other Lipid Mediat. 2015 Jan-Mar;116-117:64-75. Doi: 10.1016/J.Prostaglandins.2015.01.004. Epub 2015 Feb 14. Cyp Epoxygenase 2J2 Prevents Cardiac ...
Yang D, Gao Yh, Tan Kb, Zuo Zx, Yang Wx, Hua X, Li Pj, Zhang Y, Wang G. Gene Ther. 2013 Dec;20(12):1140-8. Doi: 10.1038/Gt.2013.41. Epub 2013 Aug 22. Inhibition Of Hepatic Fibrosis With Artificial Microrna Using Ultrasound And Cationic Liposome-Be...
Zhou X, Zhang J, Xu C, Wang W. J Pharmacol Sci. 2014;126(4):344-50. Doi: 10.1254/Jphs.14173Fp. Epub 2014 Dec 2. Curcumin Ameliorates Renal Fibrosis By Inhibiting Local Fibroblast Proliferation And Extracellular Matrix Deposition.
Yu M, Huang C, Huang Y, Wu X, Li X, Li J. Inflamm Res. 2013 Nov;62(11):961-70. Doi: 10.1007/S00011-013-0653-9. Epub 2013 Aug 11. Inhibition Of Trpm7 Channels Prevents Proliferation And Differentiation Of Human Lung Fibroblasts.
Chen F, Wan H, Xia T, Guo X, Wang H, Liu Y, Li X. Eur J Pharm Biopharm. 2013 Nov;85(3 Pt A):699-710. Doi: 10.1016/J.Ejpb.2013.07.009. Epub 2013 Jul 24. Promoted Regeneration Of Mature Blood Vessels By Electrospun Fibers With Loaded Multiple Pdna-C...
Zunwen L, Shizhen Z, Dewu L, Yungui M, Pu N. Burns. 2012 May;38(3):404-13. Doi: 10.1016/J.Burns.2011.08.013. Epub 2011 Sep 16. Effect Of Tetrandrine On The Tgf-??-Induced Smad Signal Transduction Pathway In Human Hypertrophic Scar Fibroblasts In Vi...
Li X, Han J, Li L, Wang Kj, Hu Sj. Int J Cardiol. 2013 Oct 9;168(4):3340-7. Doi: 10.1016/J.Ijcard.2013.04.038. Epub 2013 May 9. Effect Of Farnesyltransferase Inhibition On Cardiac Remodeling In Spontaneously Hypertensive Rats.
Han L, Wang L, Wang Q, Li H, Zang H. Exp Ther Med. 2014 May;7(5):1337-1341. Epub 2014 Feb 20. Association Between Pelvic Organ Prolapse And Stress Urinary Incontinence With Collagen.
Zhang Y, Zhang Y, Xie Y, Gao Y, Ma J, Yuan J, Li J, Wang J, Li L, Zhang J, Chu L. J Ethnopharmacol. 2013 Jul 9;148(2):671-81. Doi: 10.1016/J.Jep.2013.05.028. Epub 2013 May 22. Multitargeted Inhibition Of Hepatic Fibrosis In Chronic Iron-Overloaded...
Li Wx, Kong X, Zhang Jx, Yang Jr. Food Funct. 2013 Feb 26;4(3):453-60. Doi: 10.1039/C2Fo30220A. Long-Term Intake Of Sesamin Improves Left Ventricular Remodelling In Spontaneously Hypertensive Rats.
Zhang H, Teng X, Liu Z, Zhang L, Liu Z. J Exp Clin Cancer Res. 2015 Feb 12;34:16. Doi: 10.1186/S13046-015-0132-Y. Gene Expression Profile Analyze The Molecular Mechanism Of Cxcr7 Regulating Papillary Thyroid Carcinoma Growth And Metastasis.
Lv C, Wu C, Zhou Yh, Shao Y, Wang G, Wang Qy. Int J Endocrinol. 2014;2014:658589. Doi: 10.1155/2014/658589. Epub 2014 Oct 30. Alpha Lipoic Acid Modulated High Glucose-Induced Rat Mesangial Cell Dysfunction Via Mtor/P70S6K/4E-Bp1 Pathway.

FAQs

Q: Do you offer BSA-free antibodies? Keyword: Bovine serum albumin, carrier protein, conjugation
A: Yes, please contact us at support@bosterbio.com for more information about BSA-free antibodies and availability. The new BSA-free formula uses trehalose as a replacement to BSA. We have tested many alternative chemicals and found that trehalose protects the antibodies the best.
Q: Is your western blot protocol provided from the website applicable for all your antibodies? Keyword: applications, WB
A: The protocol is applicable for all our antibodies in WB, the NC Membrane(0.45μm or 0.22μm) and transfer time(70 mins or 50 mins) depends on the protein molecular weight, details can be found in included protocol.
Q: Can I conjugate markers to this antibody? Can I link custom conjugates to this antibody? Keyword: conjugation
A: The antibody is stored with BSA and cannot be conjugated with markers. Carrier free antibodies are available upon request. Please contact support@bosterbio.com
Q: What should I use for negative control?
A: Please contact us for negative control suggestions. You can also check expression databases such as genecards, uniprot etc. Due to logistic reasons, we do not sell serum or lysates that we use internally for positive or negative control.
Q: Where can I find troubleshooting information? What should I do if I have unexpected bands, high background, no signal, weak signal
A: You can find Boster's troubleshoot guides under tech support tab. Please contact us for further assistance on troubleshooting your experiment.
Q: What is the immunogen sequence of this antibody? Is this antibody polyclonal or monoclonal?
A: You can find the immunogen sequence under "Immunogen" and clonality in the product name.
Q: What is the expected band size? Why is it different than the observed band size?
A: The expected band size is predicted on the size of the protein. The actual band size may be affected by a few other factors including but not limited to:
1. Post-translational modification:phosphorylation, methylation, glycosylation etc. These modifications prevent SDS molecules from binding to the target protein and thus make the band size appear larger than expected
2. Post-translational cleavage: this can cause smaller bands and or multiple bands

3. Alternative splicing: the same gene can have alternative splicing patterns generating different size proteins, all with reactivities to the antibody.

4. Amino Acid R chain charge: SDS binds to positive charges. The different size and charge of the Amino Acid side chains can affect the amount of SDS binding and thus affect the observed band size.
5. Multimers: Multimers are usually broken up in reducing conditions. However if the interactions between the multimers are strong, the band may appear higher.,
Q: What is the suggested dilution ratio for Western Blot (WB), Immunohistochemistry (IHC) and or ELISA standards? What is the optimal pH for the sample?
A: Check the datasheet for the product for details on dilution ratios for different experiments. You can find the datasheet button on the right side of the product page.
Q: What is the protocol you used for your Western blotting (WB) and Immunohistochemistry (IHC)?
A: Check our protocols under the tech support tab.
Q: What are some alternative names that could be used to describe this product?
A: Some common names include but are not limited to col1a1 antibody, col1a2 antibody, collagen type 1 antibody