Anti-Collagen I/COL1A1 Antibody Picoband™

COL1A1 antibody

Boster Bio Anti-Collagen I/COL1A1 Antibody Picoband™ catalog # PB9939. Tested in IHC, WB applications. This antibody reacts with Mouse, Rat. Cited in 10 publication(s).

Product Info Summary

SKU: PB9939
Size: 100 μg/vial
Reactive Species: Mouse, Rat
Host: Rabbit
Application: IHC, WB

Product Name

Anti-Collagen I/COL1A1 Antibody Picoband™

View all COL1A1 Antibodies

SKU/Catalog Number

PB9939

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-Collagen I/COL1A1 Antibody Picoband™ catalog # PB9939. Tested in IHC, WB applications. This antibody reacts with Mouse, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Collagen I/COL1A1 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9939)

Host

Rabbit

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2mg Na2HPO4.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of mouse Collagen I, different from the related human sequence by three amino acids, and identical to the related rat sequence.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins

Reactive Species

PB9939 is reactive to COL1A1 in Mouse, Rat

Applications

PB9939 is guaranteed for IHC, WB Boster Guarantee

Observed Molecular Weight

138-180 kDa

Calculated molecular weight

138.941kDa

Background of COL1A1

Collagen, type I, alpha 1, also known as COL1A1, is a human gene that encodes the major component of type I collagen, the fibrillar collagen found in most connective tissues, including cartilage. This gene is mapped to 17q21.33. And this gene encodes the pro-alpha1 chains of type I collagen whose triple helix comprises two alpha1 chains and one alpha2 chain. Type I is a fibril-forming collagen found in most connective tissues and is abundant in bone, cornea, dermis and tendon. Mutations in this gene are associated with osteogenesis imperfecta types I-IV, Ehlers-Danlos syndrome type VIIA, Ehlers-Danlos syndrome Classical type, Caffey Disease and idiopathic osteoporosis.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Innovating Scientists Reward

If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Mouse, Rat, By Heat
Western blot, 0.1-0.5μg/ml, Mouse

Validation Images & Assay Conditions

Gene/Protein Information For COL1A1 (Source: Uniprot.org, NCBI)

Gene Name

COL1A1

Full Name

Collagen alpha-1(I) chain

Weight

138.941kDa

Superfamily

fibrillar collagen family

Alternative Names

Alpha-1 type I collagen; COL1A1; Collagen 1 alpha 1; Collagen 1; collagen alpha 1 chain type I; collagen alpha-1(I) chain; Collagen I alpha 1; collagen of skin, tendon and bone, alpha-1 chain; collagen, type I, alpha 1; Collagen1; Collagen-1; OI4; pro-alpha-1 collagen type 1 COL1A1 CAFYD, EDSARTH1, EDSC, OI1, OI2, OI3, OI4 collagen type I alpha 1 chain collagen alpha-1(I) chain|alpha-1 type I collagen|alpha1(I) procollagen|collagen alpha 1 chain type I|collagen alpha-1(I) chain preproprotein|collagen of skin, tendon and bone, alpha-1 chain|collagen, type I, alpha 1|pro-alpha-1 collagen type 1|type I proalpha 1|type I procollagen alpha 1 chain

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on COL1A1, check out the COL1A1 Infographic

COL1A1 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for COL1A1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

Hello CJ!

PB9939 has been cited in 10 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Investigating the antifibrotic effect of the antiparasitic drug Praziquantel in in vitro and in vivo preclinical models

Wei X,Bao Y,Zhan X,Zhang L,Hao G,Zhou J,Chen Q.MiR-200a ameliorates peritoneal fibrosis and functional deterioration in a rat model of peritoneal dialysis.Int Urol Nephrol.2019 May;51(5):889-896.doi:10.1007/s11255-019-02122-4.Epub 2019 Mar 19.PMID:30888602;PMCID:PMC6499761.
Species: Rat
PB9939 usage in article: APP:WB, SAMPLE:PERITONEAL TISSUE, DILUTION:NA

Li X,Bu X,Yan F,Wang F,Wei D,Yuan J,Zheng W,Su J,Yuan J.Deletion of discoidin domain receptor 2 attenuates renal interstitial fibrosis in a murine unilateral ureteral obstruction model.Ren Fail.2019 Nov;41(1):481-488.doi:10.1080/0886022X.2019.1621759.PMID:31169440;PMCID:PMC6567249.
Species: Mouse
PB9939 usage in article: APP:WB, SAMPLE:KIDNEY TISSUE, DILUTION:NA

Ge Y,Cheng R,Sun S,Zhang S,Li L,Jiang J,Yang C,Xuan X,Chen J.Fangxiao Formula alleviates airway inflammation and remodeling in rats with asthma via suppression of transforming growth factor -β/Smad3 signaling pathway.Biomed Pharmacother.2019 Nov;119:109429.doi:10.1016/j.biopha.2019.109429.Epub 2019 Sep 7.PMID:31505422.
Species: Rat
PB9939 usage in article: APP:IHC, SAMPLE:LUNG TISSUE, DILUTION:NA

Feng Y,Dong H,Sun B,Hu Y,Yang Y,Jia Y,Jia L,Zhong X,Zhao R.METTL3/METTL14 transactivation and m6A-dependent TGF-β1 translation in activated Kupffer cells.Cell Mol Gastroenterol Hepatol.2021 May 13:S2352-345X(21)00096-5.doi:10.1016/j.jcmgh.2021.05.007.Epub ahead of print.PMID:33992834.
Species: Human,Mouse,Rat
PB9939 usage in article: APP:WB, SAMPLE:LIVER TISSUE, DILUTION:1:500

Lan Chen,Xiaofang Ji,Manni Wang et al.Involvement of TLR4 Signaling Regulated-COX2/PGE2 Axis in Liver Fibrosis Induced by Schistosoma japonicum Infection, 06 January 2021, PREPRINT (Version 1) available at Research Square [https://doi.org/10.21203/rs.3.rs
Species: Human,Mouse
PB9939 usage in article: APP:IHC, SAMPLE:LIVER TISSUE, DILUTION:1:400

Ma XL,Ding Y,Wu LM,Wang YX,Yao Y,Wang YX,Zhang YG,Niu JQ,He XX,Wang YQ.The glucagon-like peptide-1 (GLP-1) analog exenatide ameliorates intrauterine adhesions in mice.Peptides.2021 Jan 12;137:170481.doi:10.1016/j.peptides.2020.170481.Epub ahead of print.P
Species: Mouse
PB9939 usage in article: APP:WB, SAMPLE:IUA TISSUE, DILUTION:1:1000

Nicotinamide Supplementation Attenuates Renal Interstitial Fibrosis via Boosting the Activity of Sirtuins. Xin Zhen,Shaowu Zhang,Feifei Xie,Miaomiao Zhou,Zheng Hu,Fengxin Zhu,Jing Nie
Species: Mouse
PB9939 usage in article: APP:WB, SAMPLE:KIDNEY TISSUE AND PTCS, DILUTION:NA

Francesca Lombardi,Paola Palumbo,Francesca Rosaria Augello,Ilaria Giusti,Vincenza Dolo,Luca Guerrini,Maria Grazia Cifone,Maurizio Giuliani,Benedetta Cinque,"Type I Collagen Suspension Induces Neocollagenesis and Myodifferentiation in Fibroblasts In Vitro"
Species: Mouse
PB9939 usage in article: APP:IF, SAMPLE:NIH 3T3 CELL, DILUTION:1:250

Pei-pei Fang,Chen-wei Pan,Wei Lin,Jie Li,Shan-shan Huang,Guang-yao Zhou,Wen-jun Du,Qiang Li, "ASK1 Enhances Angiotensin II-Induced Liver Fibrosis In Vitro by Mediating Endoplasmic Reticulum Stress-Dependent Exosomes",Mediators of Inflammation,vol.2020,Art
Species: Human
PB9939 usage in article: APP:WB, SAMPLE:LX-2 CELLS, DILUTION:1:1000

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16 Customer Q&As for Anti-Collagen I/COL1A1 Antibody Picoband™

Question

I would like using your anti-Collagen I/COL1A1 antibody for response to estradiol studies. Has this antibody been tested with western blotting on mouse lung? We would like to see some validation images before ordering.

Verified Customer

Verified customer

Asked: 2020-04-08

Answer

Thanks for your inquiry. This PB9939 anti-Collagen I/COL1A1 antibody is validated on mouse lung. It is guaranteed to work for IHC, WB in mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.

Boster Scientific Support

Answered: 2020-04-08

Question

I have a question about product PB9939, anti-Collagen I/COL1A1 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

Verified Customer

Verified customer

Asked: 2020-03-27

Answer

We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free PB9939 anti-Collagen I/COL1A1 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2020-03-27

Question

Will anti-Collagen I/COL1A1 antibody PB9939 work for IHC with fetal brain cortex?

Verified Customer

Verified customer

Asked: 2019-09-02

Answer

According to the expression profile of fetal brain cortex, COL1A1 is highly expressed in fetal brain cortex. So, it is likely that anti-Collagen I/COL1A1 antibody PB9939 will work for IHC with fetal brain cortex.

Boster Scientific Support

Answered: 2019-09-02

Question

See below the WB image, lot number and protocol we used for fetal brain cortex using anti-Collagen I/COL1A1 antibody PB9939. Please let me know if you require anything else.

Verified Customer

Verified customer

Asked: 2019-08-07

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2019-08-07

Question

Do you have a BSA free version of anti-Collagen I/COL1A1 antibody PB9939 available?

Verified Customer

Verified customer

Asked: 2019-07-03

Answer

Thank you for your recent telephone inquiry. I can confirm that some lots of this anti-Collagen I/COL1A1 antibody PB9939 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2019-07-03

Question

We were happy with the WB result of your anti-Collagen I/COL1A1 antibody. However we have been able to see positive staining in fetal brain cortex extracellular using this antibody. Is that expected? Could you tell me where is COL1A1 supposed to be expressed?

Verified Customer

Verified customer

Asked: 2019-03-11

Answer

According to literature, fetal brain cortex does express COL1A1. Generally COL1A1 expresses in secreted, extracellular space, extracellular. Regarding which tissues have COL1A1 expression, here are a few articles citing expression in various tissues:
Bone, Pubmed ID: 3340531
Brain, Pubmed ID: 15489334
Liver, Pubmed ID: 24275569
Skin, Pubmed ID: 4319110, 5529814

Boster Scientific Support

Answered: 2019-03-11

Question

We are currently using anti-Collagen I/COL1A1 antibody PB9939 for rat tissue, and we are satisfied with the IHC results. The species of reactivity given in the datasheet says mouse, rat. Is it true that the antibody can work on zebrafish tissues as well?

Verified Customer

Verified customer

Asked: 2019-02-05

Answer

The anti-Collagen I/COL1A1 antibody (PB9939) has not been tested for cross reactivity specifically with zebrafish tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in zebrafish you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2019-02-05

Question

I see that the anti-Collagen I/COL1A1 antibody PB9939 works with IHC, what is the protocol used to produce the result images on the product page?

A. Krishna

Verified customer

Asked: 2019-01-11

Answer

You can find protocols for IHC on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2019-01-11

Question

I was wanting to use your anti-Collagen I/COL1A1 antibody for IHC for rat fetal brain cortex on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for rat fetal brain cortex identification?

Verified Customer

Verified customer

Asked: 2018-09-12

Answer

It shows on the product datasheet, PB9939 anti-Collagen I/COL1A1 antibody has been validated for IHC, WB on mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in rat fetal brain cortex in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2018-09-12

Question

Is this PB9939 anti-Collagen I/COL1A1 antibody reactive to the isotypes of COL1A1?

G. Collins

Verified customer

Asked: 2018-04-03

Answer

The immunogen of PB9939 anti-Collagen I/COL1A1 antibody is A synthetic peptide corresponding to a sequence at the C-terminus of mouse Collagen I (1185-1207aa YDFSFLPQPPQEKSQDGGRYYRA), different from the related human sequence by three amino acids, and identical to the related rat sequence. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2018-04-03

Question

We are interested in to test anti-Collagen I/COL1A1 antibody PB9939 on rat fetal brain cortex for research purposes, then I may be interested in using anti-Collagen I/COL1A1 antibody PB9939 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

Verified Customer

Verified customer

Asked: 2017-11-06

Answer

The products we sell, including anti-Collagen I/COL1A1 antibody PB9939, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2017-11-06

Question

Is a blocking peptide available for product anti-Collagen I/COL1A1 antibody (PB9939)?

Verified Customer

Verified customer

Asked: 2017-05-15

Answer

We do provide the blocking peptide for product anti-Collagen I/COL1A1 antibody (PB9939). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2017-05-15

Question

We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for fetal brain cortex using anti-Collagen I/COL1A1 antibody PB9939. Let me know if you need anything else.

D. Edwards

Verified customer

Asked: 2016-06-29

Answer

I appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2016-06-29

Question

We have been able to see staining in rat bone. What should we do? Is anti-Collagen I/COL1A1 antibody supposed to stain bone positively?

D. Johnson

Verified customer

Asked: 2014-04-01

Answer

According to literature bone does express COL1A1. According to Uniprot.org, COL1A1 is expressed in spinal cord, spleen, brain, skin, fetal brain cortex, bone, liver, among other tissues. Regarding which tissues have COL1A1 expression, here are a few articles citing expression in various tissues:
Bone, Pubmed ID: 3340531
Brain, Pubmed ID: 15489334
Liver, Pubmed ID: 24275569
Skin, Pubmed ID: 4319110, 5529814

Boster Scientific Support

Answered: 2014-04-01

Question

We ordered your anti-Collagen I/COL1A1 antibody for WB on skin last year. I am using mouse, and We want to use the antibody for IHC next. My question regards examining skin as well as spinal cord in our next experiment. Do you have any suggestion on which antibody would work the best for IHC?

H. Zhao

Verified customer

Asked: 2013-10-28

Answer

I took a look at the website and datasheets of our anti-Collagen I/COL1A1 antibody and it appears that PB9939 has been validated on mouse in both WB and IHC. Thus PB9939 should work for your application. Our Boster satisfaction guarantee will cover this product for IHC in mouse even if the specific tissue type has not been validated. We do have a comprehensive range of products for IHC detection and you can check out our website bosterbio.com to find out more information about them.

Boster Scientific Support

Answered: 2013-10-28

Question

Will PB9939 anti-Collagen I/COL1A1 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

R. Moore

Verified customer

Asked: 2013-06-07

Answer

You can see on the product datasheet, PB9939 anti-Collagen I/COL1A1 antibody as been tested on IHC. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2013-06-07

Order DetailsPrice
PB9939

100μg

$370
PB9939-10ug

10μg sample (liquid)

$99
PB9939-Biotin

100 μg Biotin conjugated

$570
PB9939-Cy3

100 μg Cy3 conjugated

$570
PB9939-Dylight488

100 μg Dylight488 conjugated

$570
PB9939-Dylight550

100 μg Dylight550 conjugated

$570
PB9939-Dylight594

100 μg Dylight594 conjugated

$570
PB9939-FITC

100 μg FITC conjugated

$570
PB9939-HRP

100 μg HRP conjugated

$570
PB9939-APC

100 μg APC conjugated

$670
PB9939-PE

100 μg PE conjugated

$670
PB9939-carrier-free

Carrier Free

$370

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PB9939
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00

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