Anti-Collagen I COL1A1 Rabbit Monoclonal Antibody

COL1A1 antibody

Boster Bio Anti-Collagen I COL1A1 Rabbit Monoclonal Antibody catalog # M00624. Tested in WB, IHC applications. This antibody reacts with Human.

Product Info Summary

SKU: M00624
Size: 100 μl
Reactive Species: Human
Host: Rabbit
Application: IHC, WB

Product Name

Anti-Collagen I COL1A1 Rabbit Monoclonal Antibody

View all COL1A1 Antibodies

SKU/Catalog Number

M00624

Size

100 μl

Form

Liquid

Description

Boster Bio Anti-Collagen I COL1A1 Rabbit Monoclonal Antibody catalog # M00624. Tested in WB, IHC applications. This antibody reacts with Human.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Collagen I COL1A1 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00624)

Host

Rabbit

Contents

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.

Clonality

Monoclonal

Clone Number

AFD-3

Isotype

Rabbit IgG

Immunogen

A synthesized peptide derived from human Collagen I

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

Detects ~20kDa. Does not cross-react with αB-crystallin, βL-crystallin, ΒH- crystallin, γ-crystallin, HSP25, HSP27 or HSP47 proteins.

Reactive Species

M00624 is reactive to COL1A1 in Human

Applications

M00624 is guaranteed for IHC, WB Boster Guarantee

Observed Molecular Weight

26 kDa

Calculated molecular weight

138.941kDa

Background of COL1A1

Dynamin-related GTPase required for mitochondrial fusion and regulation of apoptosis. May form a diffusion barrier for proteins stored in mitochondrial cristae. Proteolytic processing in response to intrinsic apoptotic signals may lead to disassembly of OPA1 oligomers and release of the caspase activator cytochrome C (CYCS) into the mitochondrial intermembrane space.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Innovating Scientists Reward

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Reconsitution

Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB 1:500-1:2000
IHC 1:50-1:200

Validation Images & Assay Conditions

Gene/Protein Information For COL1A1 (Source: Uniprot.org, NCBI)

Gene Name

COL1A1

Full Name

Collagen alpha-1(I) chain

Weight

138.941kDa

Superfamily

fibrillar collagen family

Alternative Names

Alpha-1 type I collagen; COL1A1; Collagen 1 alpha 1; Collagen 1; collagen alpha 1 chain type I; collagen alpha-1(I) chain; Collagen I alpha 1; collagen of skin, tendon and bone, alpha-1 chain; collagen, type I, alpha 1; Collagen1; Collagen-1; OI4; pro-alpha-1 collagen type 1 COL1A1 CAFYD, EDSARTH1, EDSC, OI1, OI2, OI3, OI4 collagen type I alpha 1 chain collagen alpha-1(I) chain|alpha-1 type I collagen|alpha1(I) procollagen|collagen alpha 1 chain type I|collagen alpha-1(I) chain preproprotein|collagen of skin, tendon and bone, alpha-1 chain|collagen, type I, alpha 1|pro-alpha-1 collagen type 1|type I proalpha 1|type I procollagen alpha 1 chain

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on COL1A1, check out the COL1A1 Infographic

COL1A1 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for COL1A1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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1 Customer Q&As for Anti-Collagen I COL1A1 Rabbit Monoclonal Antibody

Question

We are currently using anti-Collagen I Rabbit Monoclonal antibody M00624 for human tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human. Is it possible that the antibody can work on primate tissues as well?

Verified Customer

Verified customer

Asked: 2020-01-23

Answer

The anti-Collagen I Rabbit Monoclonal antibody (M00624) has not been validated for cross reactivity specifically with primate tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in primate you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2020-01-23

Order DetailsPrice
M00624

100μL

$370
M00624-30ul

30μL sample

$99
M00624-carrier-free

Carrier Free

$370

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M00624
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00

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