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SKU:PA2141
Clonality:Polyclonal
Application:WB
Price: $200.00
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Overview

Product Name Anti-Collagen II Antibody
Description Rabbit IgG polyclonal antibody for Collagen alpha-1(II) chain(COL2A1) detection. Tested with WB in Human;Mouse;Rat.
Cite This Product Anti-Collagen II Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA2141)
Replacement Item This antibody may replace the following items: sc-28887|sc-7764|sc-52658|sc-7763 from Santa Cruz Biotechnology.
Host Rabbit
Isotype N/A
Validated Species Human, Mouse, Rat
Predicted Species Hamster

*This antibody is predicted to react with the above species based on antigen sequence similarities. Our Boster Guarantee covers the use of this product with the above species.

Application WB

*Our Boster Guarantee covers the use of this product in the above tested applications.

**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.

Recommended Detection Systems Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot.
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2017!
Immunogen A synthetic peptide corresponding to a sequence at the C-terminus of human Collagen II(1222-1241aa AFAGLGPREKGPDPLQYMRA), different from the related rat sequence by one amino acid, and from the related mouse sequence by two amino acids.
Cross Reactivity No cross reactivity with other proteins
Pack Size 100μg/vial

Properties

Clonality Polyclonal
Form Lyophilized
Contents Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
*carrier free antibody available upon request.
Concentration Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Storage At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing.
Purification Immunogen affinity purified.
Isotype N/A

Protein Target Info (Source: Uniprot.org)

You can check the tissue specificity below for information on selecting positive and negative control.

Gene Name COL2A1
Protein Name Collagen alpha-1(II) chain
Molecular Weight 141785 MW
Protein Function Type II collagen is specific for cartilaginous tissues. It is essential for the normal embryonic development of the skeleton, for linear growth and for the ability of cartilage to resist compressive forces.
Tissue Specificity Isoform 2 is highly expressed in juvenile chondrocyte and low in fetal chondrocyte. .
Sequence Similarities Belongs to the fibrillar collagen family.
Subcellular Localization Secreted, extracellular space, extracellular matrix .
Uniprot ID P02458
Alternative Names Collagen alpha-1(II) chain;Alpha-1 type II collagen;Collagen alpha-1(II) chain;Chondrocalcin;COL2A1;
Research Areas |signal transduction|cytoskeleton / ecm|extracellular matrix|ecm proteins|collagen| signal transduction|structures|bone| stem cells|mesenchymal stem cells|chondrogenesis|
*if product is indicated to react with multiple species, protein info is based on the human gene.

Background for Collagen alpha-1(II) chain

Collagen, type II, alpha 1, also known as COL2A1, is a human gene which is the major collagen synthesized by chondrocvtes. COL2A1 is mapped to 12q13.11. This gene encodes the alpha-1 chain of type II collagen, a fibrillar collagen found in cartilage and the vitreous humor of the eye. Mutations in this gene are associated with achondrogenesis, chondrodysplasia, early onset familial osteoarthritis, SED congenita, Langer-Saldino achondrogenesis, Kniest dysplasia, Stickler syndrome type I, and spondyloepimetaphyseal dysplasia Strudwick type. In addition, defects in processing chondrocalcin, a calcium binding protein that is the C-propeptide of this collagen molecule, are also associated with chondrodysplasia.

Anti-Collagen II Antibody Images

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Anti-Collagen II Antibody
Anti- COL2A1 antibody, PA2141, Western blotting
All lanes: Anti COL2A1 (PA2141) at 0.5ug/ml
Lane 1: COLO320 Whole Cell Lysate at 40ug
Lane 2: HELA Whole Cell Lysate at 40ug
Lane 3: MCF-7Whole Cell Lysate at 40ug
Lane 4: A549 Whole Cell Lysate at 40ug
Lane 5: JURKAT Whole Cell Lysate at 40ug
Predicted bind size: 134 KD
Observed bind size: 170KD
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Publications

Chen H, Yang X, Liao Y, Zeng X, Liang P, Kang N, Tan J, Liang Z. J Biomed Mater Res B Appl Biomater. 2011 Feb;96(2):267-75. Doi: 10.1002/Jbm.B.31762. Epub 2010 Nov 17. Mri And Histologic Analysis Of Collagen Type Ii Sponge On Repairing The Cartila...
Li Jw, Guo Xl, He Cl, Tuo Yh, Wang Z, Wen J, Jin D. Chin Med J (Engl). 2011 Oct;124(19):3080-6. In Vitro Chondrogenesis Of The Goat Bone Marrow Mesenchymal Stem Cells Directed By Chondrocytes In Monolayer And 3-Dimetional Indirect Co-Culture System.
You M, Peng G, Li J, Ma P, Wang Z, Shu W, Peng S, Chen Gq. Biomaterials. 2011 Mar;32(9):2305-13. Doi: 10.1016/J.Biomaterials.2010.12.009. Epub 2010 Dec 28. Chondrogenic Differentiation Of Human Bone Marrow Mesenchymal Stem Cells On Polyhydroxyalka...
Li H, Yang Hs, Wu Tj, Zhang Xy, Jiang Wh, Ma Ql, Chen Yx, Xu Y, Li S, Hua Zc. J Cell Physiol. 2010 Jun;223(3):610-22. Doi: 10.1002/Jcp.22052. Proteomic Analysis Of Early-Response To Mechanical Stress In Neonatal Rat Mandibular Condylar Chondrocytes.
Li J, Wang J, Zou Y, Zhang Y, Long D, Lei L, Tan L, Ye R, Wang X, Zhao Z. Biomaterials. 2012 Nov;33(33):8395-405. Doi: 10.1016/J.Biomaterials.2012.08.019. Epub 2012 Aug 24. The Influence Of Delayed Compressive Stress On Tgf-??1-Induced Chondrogenic...
Ye R, Hao J, Song J, Zhao Z, Fang S, Wang Y, Li J. J Cell Biochem. 2014 Jun;115(6):1089-96. Doi: 10.1002/Jcb.24743. Microenvironment Is Involved In Cellular Response To Hydrostatic Pressures During Chondrogenesis Of Mesenchymal Stem Cells.
Zhang W, Zhang F, Shi H, Tan R, Han S, Ye G, Pan S, Sun F, Liu X. Plos One. 2014 Feb 18;9(2):E88794. Doi: 10.1371/Journal.Pone.0088794. Ecollection 2014. Comparisons Of Rabbit Bone Marrow Mesenchymal Stem Cell Isolation And Culture Methods In Vitro.
Han Zc, Zhang Hn, Wang Yz, Lv Cy, Xu Zy. Saudi Med J. 2014 May;35(5):435-41. Effect Of The Human Insulin-Like Growth Factor 1 Gene Transfection To Human Umbilical Cord Blood Mesenchymal Stem Cells.
Qian J, Liang J, Wang Y, Wang H. Exp Ther Med. 2014 Aug;8(2):377-383. Epub 2014 May 29. Effect Of Passive Motion On Articular Cartilage In Rat Osteoarthritis.
Li X, Du M, Liu X, Wu M, Ye H, Lin J, Chen W, Wu G. Int J Mol Med. 2010 Mar;25(3):393-9. Millimeter Wave Treatment Inhibits No-Induced Apoptosis Of Chondrocytes Through The P38Mapk Pathway.
Lin Q, Fu Q, Zhang Y, Wang H, Liu Z, Zhou J, Duan C, Wang Y, Wu K, Wang C. Eur J Heart Fail. 2010 Nov;12(11):1179-85. Doi: 10.1093/Eurjhf/Hfq144. Epub 2010 Sep 3. Tumourigenesis In The Infarcted Rat Heart Is Eliminated Through Differentiation And ...
Cai B, Zhang Ag, Zhang X, Ge Wj, Dai Gd, Tan Xl, Roodrajeetsing G, Cai Jp. Biomed Res Int. 2015;2015:792161. Doi: 10.1155/2015/792161. Epub 2015 Jun 7. Promoting Effects On Proliferation And Chondrogenic Differentiation Of Bone Marrow-Derived Mese...
Ye J, Wu G, Li X, Li Z, Zheng C, Liu X, Ye H. Evid Based Complement Alternat Med. 2015;2015:464161. Doi: 10.1155/2015/464161. Epub 2015 Jan 29. Millimeter Wave Treatment Inhibits Apoptosis Of Chondrocytes Via Regulation Dynamic Equilibrium Of Intr...
Zhang Lf, Ding Wh, Shi Lb, Li K, Haom Yj, Ke Yn, Tang Zs. Clin Exp Pharmacol Physiol. 2010 Apr;37(4):477-81. Doi: 10.1111/J.1440-1681.2009.05336.X. Epub 2009 Nov 23. Effects Of Exogenous Urotensin Ii On Vascular Remodelling After Balloon Injury.
Fu Z, Liu P, Yang D, Wang F, Yuan L, Lin Z, Jiang J. Int J Mol Med. 2012 Oct;30(4):805-10. Doi: 10.3892/Ijmm.2012.1073. Epub 2012 Jul 20. Interleukin-18-Induced Inflammatory Responses In Synoviocytes And Chondrocytes From Osteoarthriti...
Liu L, Liu Q, Lin X, Wei Qj, Zheng L. In Vitro Cell Dev Biol Anim. 2014 Dec;50(10):982-91. Doi: 10.1007/S11626-014-9795-5. Epub 2014 Aug 15. Effect Of Jeztc, A Synthetic Compound, On Proliferation And Phenotype Maintenance Of Rabbit Articular Chon...
Liu Q, Lu Z, Wu H, Zheng L. Tohoku J Exp Med. 2015;235(3):201-13. Doi: 10.1620/Tjem.235.201. Chondroprotective Effects Of Taurine In Primary Cultures Of Human Articular Chondrocytes.

FAQs

Q: Do you offer BSA-free antibodies? Keyword: Bovine serum albumin, carrier protein, conjugation
A: Yes, please contact us at support@bosterbio.com for more information about BSA-free antibodies and availability. The new BSA-free formula uses trehalose as a replacement to BSA. We have tested many alternative chemicals and found that trehalose protects the antibodies the best.
Q: Is your western blot protocol provided from the website applicable for all your antibodies? Keyword: applications, WB
A: The protocol is applicable for all our antibodies in WB, the NC Membrane(0.45μm or 0.22μm) and transfer time(70 mins or 50 mins) depends on the protein molecular weight, details can be found in included protocol.
Q: Can I conjugate markers to this antibody? Can I link custom conjugates to this antibody? Keyword: conjugation
A: The antibody is stored with BSA and cannot be conjugated with markers. Carrier free antibodies are available upon request. Please contact support@bosterbio.com
Q: What should I use for negative control?
A: Please contact us for negative control suggestions. You can also check expression databases such as genecards, uniprot etc. Due to logistic reasons, we do not sell serum or lysates that we use internally for positive or negative control.
Q: Where can I find troubleshooting information? What should I do if I have unexpected bands, high background, no signal, weak signal
A: You can find Boster's troubleshoot guides under tech support tab. Please contact us for further assistance on troubleshooting your experiment.
Q: What is the immunogen sequence of this antibody? Is this antibody polyclonal or monoclonal?
A: You can find the immunogen sequence under "Immunogen" and clonality in the product name.
Q: What is the expected band size? Why is it different than the observed band size?
A: The expected band size is predicted on the size of the protein. The actual band size may be affected by a few other factors including but not limited to:
1. Post-translational modification:phosphorylation, methylation, glycosylation etc. These modifications prevent SDS molecules from binding to the target protein and thus make the band size appear larger than expected
2. Post-translational cleavage: this can cause smaller bands and or multiple bands

3. Alternative splicing: the same gene can have alternative splicing patterns generating different size proteins, all with reactivities to the antibody.

4. Amino Acid R chain charge: SDS binds to positive charges. The different size and charge of the Amino Acid side chains can affect the amount of SDS binding and thus affect the observed band size.
5. Multimers: Multimers are usually broken up in reducing conditions. However if the interactions between the multimers are strong, the band may appear higher.,
Q: What is the suggested dilution ratio for Western Blot (WB), Immunohistochemistry (IHC) and or ELISA standards? What is the optimal pH for the sample?
A: Check the datasheet for the product for details on dilution ratios for different experiments. You can find the datasheet button on the right side of the product page.
Q: What is the protocol you used for your Western blotting (WB) and Immunohistochemistry (IHC)?
A: Check our protocols under the tech support tab.
Q: What are some alternative names that could be used to describe this product?
A: One other very common name is col2a1 antibody
Q: What are some alternative names that could be used to describe this product?
A: One other very common name is col2a1 antibody