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SKU:RP1033
Clonality:Polyclonal
Application:WB
Price: $200.00
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Overview

Product Name Anti-Collagen III Antibody
SKU/Catalog Number RP1033
Description Rabbit IgG polyclonal antibody for Collagen alpha-1(III) chain(COL3A1) detection. Tested with WB in Human.
Cite This Product Anti-Collagen III Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # RP1033)
Replacement Item This antibody may replace the following items: sc-28888|sc-8781|sc-271249|sc-8780-R|sc-514601 from Santa Cruz Biotechnology.
Host Rabbit
Isotype N/A
Validated Species Human
Application WB

*Our Boster Guarantee covers the use of this product in the above tested applications.

**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.

Recommended Detection Systems Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot.
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2017!
Immunogen A synthetic peptide corresponding to a sequence at the C-terminus of human Collagen III(1200-1221aa VGAAAIAGIGGEKAGGFAPYYG), different from the related mouse sequence by four amino acids, and from the related rat sequence by five amino acids.
Cross Reactivity No cross reactivity with other proteins
Pack Size 100μg/vial

Properties

Clonality Polyclonal
Form Lyophilized
Contents Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
*carrier free antibody available upon request.
Concentration Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Storage At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing.
Purification Immunogen affinity purified.
Isotype N/A

Protein Target Info (Source: Uniprot.org)

You can check the tissue specificity below for information on selecting positive and negative control.

Gene Name COL3A1
Protein Name Collagen alpha-1(III) chain
Molecular Weight 138564 MW
Protein Function Collagen type III occurs in most soft connective tissues along with type I collagen. Involved in regulation of cortical development. Is the major ligand of GPR56 in the developing brain and binding to GPR56 inhibits neuronal migration and activates the RhoA pathway by coupling GPR56 to GNA13 and possibly GNA12.
Sequence Similarities Belongs to the fibrillar collagen family.
Subcellular Localization Secreted, extracellular space, extracellular matrix .
Uniprot ID P02461
Alternative Names Collagen alpha-1(III) chain;COL3A1;
Research Areas |signal transduction|cytoskeleton / ecm|extracellular matrix|ecm proteins|collagen| stem cells|mesenchymal stem cells|chondrogenesis|osteogenesis|
*if product is indicated to react with multiple species, protein info is based on the human gene.

Background for Collagen alpha-1(III) chain

COL3A1, also called EDS4A or Collagen alpha-1(III), is a protein that in humans is encoded by the COL3A1 gene, it is mapped to 2q32.2. COL3A1 chain is a fibrillar-forming collagen comprising 3 alpha-1(III) chains and is expressed in early embryos and throughout embryogenesis. In adult, COL3A1 is a major component of the extracellular matrix in a variety of internal organs and skin. COL3A1 is also a fibrous scleroprotein in bone, cartilage, dentin, tendon, bone marrow stroma and other connective tissue. It is involved in regulation of cortical development, and it Is the major ligand of GPR56 in the developing brain.COL3A1 binding to GPR56 can inhibit neuronal migration and activate the RhoA pathway by coupling GPR56 to GNA13 and possibly GNA12

Anti-Collagen III Antibody Images

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Anti-Collagen III Antibody
Anti-Collagen III Picoband antibody, RP1033-1.jpg
All lanes: Anti Collagen III (RP1033) at 0.5ug/ml
WB: Recombinant Human Collagen III Protein 0.5ng
Predicted bind size: 39KD
Observed bind size: 39KD
Anti-Collagen III Antibody
Anti-Collagen III Picoband antibody, RP1033-2.jpg
All lanes: Anti Collagen III(RP1033) at 0.5ug/ml
Lane 1: MCF-4 Whole Cell Lysate at 40ug
Lane 2: COLO320 Whole Cell Lysate at 40ug
Predicted bind size: 139KD
Observed bind size: 139KD
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Publications

Ge Ws, Wang Yj, Wu Jx, Fan Jg, Chen Yw, Zhu L. Mol Med Rep. 2014 Jun;9(6):2145-51. Doi: 10.3892/Mmr.2014.2099. Epub 2014 Apr 1. ??-Catenin Is Overexpressed In Hepatic Fibrosis And Blockage Of Wnt/??-Catenin Signaling Inhibits Hepatic Stellate Cell A...
Li X, Du J, Jin H, Geng B, Tang C. Heart Vessels. 2008 Nov;23(6):409-19. Doi: 10.1007/S00380-008-1059-4. Epub 2008 Nov 27. Sodium Hydrosulfide Alleviates Pulmonary Artery Collagen Remodeling In Rats With High Pulmonary Blood Flow.
Zhang Lf, Ding Wh, Shi Lb, Li K, Haom Yj, Ke Yn, Tang Zs. Clin Exp Pharmacol Physiol. 2010 Apr;37(4):477-81. Doi: 10.1111/J.1440-1681.2009.05336.X. Epub 2009 Nov 23. Effects Of Exogenous Urotensin Ii On Vascular Remodelling After Balloon Injury.
Guo C, Xu L, He Q, Liang T, Duan X, Li R. Food Chem Toxicol. 2013 Jun;56:436-42. Doi: 10.1016/J.Fct.2013.02.051. Epub 2013 Mar 14. Anti-Fibrotic Effects Of Puerarin On Ccl4-Induced Hepatic Fibrosis In Rats Possibly Through The Regulation Of Ppar-??...
Yang L, Ni L, Duan Q, Wang X, Chen C, Chen S, Chaugai S, Zeldin Dc, Tang Jr, Wang Dw. Prostaglandins Other Lipid Mediat. 2015 Jan-Mar;116-117:64-75. Doi: 10.1016/J.Prostaglandins.2015.01.004. Epub 2015 Feb 14. Cyp Epoxygenase 2J2 Prevents Cardiac ...
Zhai W, Wang N, Qi Z, Gao Q, Yi L. Orthopedics. 2012 Apr;35(4):E520-5. Doi: 10.3928/01477447-20120327-22. Platelet-Rich Plasma Reverses The Inhibition Of Tenocytes And Osteoblasts In Tendon-Bone Healing.
Xu Y, Zhang Ja, Xu Y, Guo Sl, Wang S, Wu D, Wang Y, Luo D, Zhou Br. Stem Cells Dev. 2015 May 1;24(9):1096-111. Doi: 10.1089/Scd.2014.0321. Epub 2015 Jan 21. Antiphotoaging Effect Of Conditioned Medium Of Dedifferentiated Adipocytes On Skin In Vivo...
Liang J, Deng X, Lin Zx, Zhao Lc, Zhang Xl. World J Gastroenterol. 2009 Sep 28;15(36):4529-37. Attenuation Of Portal Hypertension By Natural Taurine In Rats With Liver Cirrhosis.
Ge Ws, Wu Jx, Fan Jg, Wang Yj, Chen Yw. World J Gastroenterol. 2011 Sep 28;17(36):4090-8. Doi: 10.3748/Wjg.V17.I36.4090. Inhibition Of High-Mobility Group Box 1 Expression By Sirna In Rat Hepatic Stellate Cells.
Zunwen L, Shizhen Z, Dewu L, Yungui M, Pu N. Burns. 2012 May;38(3):404-13. Doi: 10.1016/J.Burns.2011.08.013. Epub 2011 Sep 16. Effect Of Tetrandrine On The Tgf-??-Induced Smad Signal Transduction Pathway In Human Hypertrophic Scar Fibroblasts In Vi...
Li X, Han J, Li L, Wang Kj, Hu Sj. Int J Cardiol. 2013 Oct 9;168(4):3340-7. Doi: 10.1016/J.Ijcard.2013.04.038. Epub 2013 May 9. Effect Of Farnesyltransferase Inhibition On Cardiac Remodeling In Spontaneously Hypertensive Rats.
Han L, Wang L, Wang Q, Li H, Zang H. Exp Ther Med. 2014 May;7(5):1337-1341. Epub 2014 Feb 20. Association Between Pelvic Organ Prolapse And Stress Urinary Incontinence With Collagen.
Zhang Y, Zhang Y, Xie Y, Gao Y, Ma J, Yuan J, Li J, Wang J, Li L, Zhang J, Chu L. J Ethnopharmacol. 2013 Jul 9;148(2):671-81. Doi: 10.1016/J.Jep.2013.05.028. Epub 2013 May 22. Multitargeted Inhibition Of Hepatic Fibrosis In Chronic Iron-Overloaded...

FAQs

Q: Do you offer BSA-free antibodies? Keyword: Bovine serum albumin, carrier protein, conjugation
A: Yes, please contact us at support@bosterbio.com for more information about BSA-free antibodies and availability. The new BSA-free formula uses trehalose as a replacement to BSA. We have tested many alternative chemicals and found that trehalose protects the antibodies the best.
Q: Can I conjugate markers to this antibody? Can I link custom conjugates to this antibody? Keyword: conjugation
A: The antibody is stored with BSA and cannot be conjugated with markers. Carrier free antibodies are available upon request. Please contact support@bosterbio.com
Q: What should I use for negative control?
A: Please contact us for negative control suggestions. You can also check expression databases such as genecards, uniprot etc. Due to logistic reasons, we do not sell serum or lysates that we use internally for positive or negative control.
Q: Where can I find troubleshooting information? What should I do if I have unexpected bands, high background, no signal, weak signal
A: You can find Boster's troubleshoot guides under tech support tab. Please contact us for further assistance on troubleshooting your experiment.
Q: What is the immunogen sequence of this antibody? Is this antibody polyclonal or monoclonal?
A: You can find the immunogen sequence under "Immunogen" and clonality in the product name.
Q: What is the expected band size? Why is it different than the observed band size?
A: The expected band size is predicted on the size of the protein. The actual band size may be affected by a few other factors including but not limited to:
1. Post-translational modification:phosphorylation, methylation, glycosylation etc. These modifications prevent SDS molecules from binding to the target protein and thus make the band size appear larger than expected
2. Post-translational cleavage: this can cause smaller bands and or multiple bands

3. Alternative splicing: the same gene can have alternative splicing patterns generating different size proteins, all with reactivities to the antibody.

4. Amino Acid R chain charge: SDS binds to positive charges. The different size and charge of the Amino Acid side chains can affect the amount of SDS binding and thus affect the observed band size.
5. Multimers: Multimers are usually broken up in reducing conditions. However if the interactions between the multimers are strong, the band may appear higher.,
Q: What is the suggested dilution ratio for Western Blot (WB), Immunohistochemistry (IHC) and or ELISA standards? What is the optimal pH for the sample?
A: Check the datasheet for the product for details on dilution ratios for different experiments. You can find the datasheet button on the right side of the product page.
Q: What is the protocol you used for your Western blotting (WB) and Immunohistochemistry (IHC)?
A: Check our protocols under the tech support tab.
Q: What are some alternative names that could be used to describe this product?
A: Some common names include but are not limited to col3a1 antibody, type iii collagen antibody