Anti-Canstatin Antibody

COL4A2 antibody

Boster Bio Anti-Canstatin Antibody catalog # PA1521. Tested in IF, IHC, IHC-F, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. Cited in 10 publication(s).

Product Info Summary

SKU: PA1521
Size: 100 μg/vial
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: IF, IHC, IHC-F, ICC, WB

Product Name

Anti-Canstatin Antibody

View all COL4A2 Antibodies

SKU/Catalog Number

PA1521

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-Canstatin Antibody catalog # PA1521. Tested in IF, IHC, IHC-F, ICC, WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Canstatin Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1521)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of human Canstatin, identical to the related rat and mouse sequences.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins

Reactive Species

PA1521 is reactive to COL4A2 in Human, Mouse, Rat

Applications

PA1521 is guaranteed for IF, IHC, IHC-F, ICC, WB Boster Guarantee

Observed Molecular Weight

56 kDa

Calculated molecular weight

167.553kDa

Background of COL4A2

Canstatin, a cleavage product corresponding to the collagen alpha 2 (IV) NC1 domain, possesses both anti-angiogenic and anti-tumor cell activity. It inhibits proliferation and migration of endothelial cells, reduces mitochondrial membrane potential, and induces apoptosis. Specifically induces Fas-dependent apoptosis and activates procaspase-8 and -9 activity. Ligand for alphavbeta3 and alphavbeta5 integrins.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Innovating Scientists Reward

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml, Human, Rat
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Mouse, Rat, By Heat

Immunohistochemistry (Frozen Section), 0.5-1μg/ml, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 2μg/ml, Human

Validation Images & Assay Conditions

Gene/Protein Information For COL4A2 (Source: Uniprot.org, NCBI)

Gene Name

COL4A2

Full Name

Collagen alpha-2(IV) chain

Weight

167.553kDa

Superfamily

type IV collagen family

Alternative Names

Collagen alpha-2(IV) chain COL4A2 BSVD2, ICH, POREN2 collagen type IV alpha 2 chain collagen alpha-2(IV) chain|canstatin

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on COL4A2, check out the COL4A2 Infographic

COL4A2 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for COL4A2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

Hello CJ!

PA1521 has been cited in 10 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Qi MY,Wang XT,Xu HL,Yang ZL,Cheng Y,Zhou B.Protective effect of ferulic acid on STZ-induced diabetic nephropathy in rats.Food Funct.2020 Apr 1;11(4):3706-3718.doi:10.1039/c9fo02398d.Epub 2020 Apr 20. PMID:32307498.
Species: Rat
PA1521 usage in article: APP:WB, SAMPLE:KIDNEY TISSUE, DILUTION:1:1000

Sugiyama A,Kaisho M,Okada M,Otani K,Yamawaki H.Decreased Expression of Canstatin in Rat Model of Monocrotaline-Induced Pulmonary Arterial Hypertension:Protective Effect of Canstatin on Right Ventricular Remodeling.Int J Mol Sci.2020 Sep 16;21(18):6797.doi
Species: Rat
PA1521 usage in article: APP:WB, SAMPLE:HEART VENTRICLES,LUNG TISSUE,KIDNEY TISSUE AND LIVER TISSUE, DILUTION:1:500

Zhu X, Chen Y, Chen Q, Yang H, Xie X. J Diabetes Res. 2018 Mar 21;2018:6730315. doi: 10.1155/2018/6730315. eCollection 2018. Astaxanthin Promotes Nrf2/ARE Signaling to Alleviate Renal Fibronectin and Collagen IV Accumulation in Diabetic Rats

Qiu X, Zhang Y, Zhao X, Zhang S, Wu J, Guo H, Hu Y. Biomaterials. 2015;53:600-8. Doi: 10.1016/J.Biomaterials.2015.02.115. Epub 2015 Mar 23. Enhancement Of Endothelial Differentiation Of Adipose Derived Mesenchymal Stem Cells By A Three-Dimensional...

Ji H, Wu L, Ma X, Ma X, Qin G. Mol Biol Rep. 2014;41(4):2151-9. Doi: 10.1007/S11033-014-3064-2. Epub 2014 Jan 12. The Effect Of Resveratrol On The Expression Of Adipor1 In Kidneys Of Diabetic Nephropathy.

Qi My, Kai-Chen, Liu Hr, Su Yh, Yu Sq. J Ethnopharmacol. 2011 Dec 8;138(3):731-6. Doi: 10.1016/J.Jep.2011.10.015. Epub 2011 Oct 19. Protective Effect Of Icariin On The Early Stage Of Experimental Diabetic Nephropathy Induced By Streptozotocin Via ...

Wenbin Z, Guojun G. West Indian Med J. 2014 Jan;63(1):20-5. Doi: 10.7727/Wimj.2014.008. Epub 2014 Apr 8. Resveratrol Ameliorates Diabetes-Induced Renal Damage Through Regulating The Expression Of Tgf-??1, Collagen Iv And Th17/Treg-Related Cytokine...

Luo X, Deng L, Lamsal Lp, Xu W, Xiang C, Cheng L. Cell Physiol Biochem. 2015;35(1):191-200. Doi: 10.1159/000369687. Epub 2015 Jan 2. Amp-Activated Protein Kinase Alleviates Extracellular Matrix Accumulation In High Glucose-Induced Renal Fibroblast...

Xue H, Zhang Yl, Liu Gs, Wang H. J Pharmacol Exp Ther. 2005 Nov;315(2):501-9. Epub 2005 Jul 28. A New Atp-Sensitive Potassium Channel Opener Protects The Kidney From Hypertensive Damage In Spontaneously Hypertensive Rats.

He S, Xia T, Wang H, Wei L, Luo X, Li X. Acta Biomater. 2012 Jul;8(7):2659-69. Doi: 10.1016/J.Actbio.2012.03.044. Epub 2012 Apr 3. Multiple Release Of Polyplexes Of Plasmids Vegf And Bfgf From Electrospun Fibrous Scaffolds Towards Regeneration Of ...

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2 Customer Q&As for Anti-Canstatin Antibody

Question

The predict band size is 167kd in Uniprot (https://www.uniprot.org/uniprotkb/P08572/entry#sequences),but the band size is 25-35kd for WB in your datasheet. Could you help explain it ?

Verified customer

Asked: 2022-09-28

Answer

PA1521 detected cleavage Canstatin with smaller MW. The immunogen sequence of PA1521 is 1697-1712aa.

Boster Scientific Support

Answered: 2022-09-28

Question

We are currently using anti-Collagen IV/COL4A2 antibody PA1521 for human tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on dog tissues as well?

E. Williams

Verified customer

Asked: 2019-05-08

Answer

The anti-Collagen IV/COL4A2 antibody (PA1521) has not been validated for cross reactivity specifically with dog tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in dog you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2019-05-08

Order DetailsPrice
PA1521

100μg

$370
PA1521-10ug

10μg sample (liquid)

$99
PA1521-Biotin

100 μg Biotin conjugated

$570
PA1521-Cy3

100 μg Cy3 conjugated

$570
PA1521-Dylight488

100 μg Dylight488 conjugated

$570
PA1521-Dylight550

100 μg Dylight550 conjugated

$570
PA1521-Dylight594

100 μg Dylight594 conjugated

$570
PA1521-FITC

100 μg FITC conjugated

$570
PA1521-HRP

100 μg HRP conjugated

$570
PA1521-APC

100 μg APC conjugated

$670
PA1521-PE

100 μg PE conjugated

$670
PA1521-carrier-free

Carrier Free

$370

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PA1521
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
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$370.00

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