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SKU:PA1521
Pack Size:100μg/vial
Sample Size:30ug for $99, contact us for details
Clonality:Polyclonal
Application:IHC-P, IHC-F, ICC, WB
Price: $200.00
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Overview

Product Name Anti-Collagen IV Antibody
SKU/Catalog Number PA1521
Description Rabbit IgG polyclonal antibody for Collagen alpha-2(IV) chain(COL4A2) detection. Tested with WB, IHC-P, IHC-F, ICC in Human;Mouse;Rat.
Cite This Product Anti-Collagen IV Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1521)
Replacement Item This antibody may replace the following items: sc-70246|sc-135231|sc-70244|sc-70243 from Santa Cruz Biotechnology.
Host Rabbit
Isotype N/A
Validated Species Human, Mouse, Rat
Predicted Species Hamster

*This antibody is predicted to react with the above species based on antigen sequence similarities. Our Boster Guarantee covers the use of this product with the above species.

Application IHC-P, IHC-F, ICC, WB

*Our Boster Guarantee covers the use of this product in the above tested applications.

**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.

Recommended Detection Systems Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P), IHC(F) and ICC.
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2017!
Immunogen A synthetic peptide corresponding to a sequence at the C-terminus of human Collagen IV(1697-1712aa LIRTHISRCQVCMKNL), identical to the related rat and mouse sequences.
Cross Reactivity No cross reactivity with other proteins
Pack Size 100μg/vial

Properties

Clonality Polyclonal
Form Lyophilized
Contents Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
*carrier free antibody available upon request.
Concentration Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Storage At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing.
Purification Immunogen affinity purified.
Isotype N/A

Protein Target Info (Source: Uniprot.org)

You can check the tissue specificity below for information on selecting positive and negative control.

Gene Name COL4A2
Protein Name Collagen alpha-2(IV) chain
Molecular Weight 167553 MW
Protein Function Type IV collagen is the major structural component of glomerular basement membranes (GBM), forming a 'chicken-wire' meshwork together with laminins, proteoglycans and entactin/nidogen.
Sequence Similarities Belongs to the type IV collagen family.
Subcellular Localization Secreted, extracellular space, extracellular matrix, basement membrane.
Uniprot ID P08572
Alternative Names Collagen alpha-2(IV) chain;Canstatin;COL4A2;
Research Areas |cardiovascular|angiogenesis|adhesion / ecm|extracellular matrix| signal transduction|cytoskeleton / ecm|ecm proteins|collagen|
*if product is indicated to react with multiple species, protein info is based on the human gene.

Background for Collagen alpha-2(IV) chain

Canstatin, a cleavage product corresponding to the collagen alpha 2(IV) NC1 domain, possesses both anti-angiogenic and anti-tumor cell activity. It inhibits proliferation and migration of endothelial cells, reduces mitochondrial membrane potential, and induces apoptosis. Specifically induces Fas-dependent apoptosis and activates procaspase-8 and -9 activity. Ligand for alphavbeta3 and alphavbeta5 integrins.

Anti-Collagen IV Antibody Images

Click the images to enlarge.

Anti-Collagen IV Antibody
Anti-Collagen IV antibody, PA1521, Western blotting
Lane 1: Rat Kidney Tissue Lysate
Lane 2: Rat Lung Tissue Lysate
Anti-Collagen IV Antibody
Anti-Collagen IV antibody, PA1521, IHC(F)
IHC(F): Rat Kidney Tissue
Anti-Collagen IV Antibody
Anti-Collagen IV antibody, PA1521, IHC(P)
IHC(P): Mouse Lung Tissue
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Publications

Hirokazu Urushiyama, Yasuhiro Terasaki, Shinya Nagasaka, Nariaki Kokuho, Mika Terasaki, Shinobu Kunugi Yu Mikami, Satoshi Noguchi, Masafumi Horie, Kiyotaka Nagahama, Yasuhiro Yamauchi, Akira Shimizu, Takahide Nagase1 Int J Clin Exp Pathol 2016;9(12):12714-12722 www.ijcep.com /ISSN:1936-2625/IJCEP0036531 Role of canstatin in early fibrotic lesions of idiopathic interstitial pneumonias and migration of lung fibroblasts
Qiu X, Zhang Y, Zhao X, Zhang S, Wu J, Guo H, Hu Y. Biomaterials. 2015;53:600-8. Doi: 10.1016/J.Biomaterials.2015.02.115. Epub 2015 Mar 23. Enhancement Of Endothelial Differentiation Of Adipose Derived Mesenchymal Stem Cells By A Three-Dimensional...
Ji H, Wu L, Ma X, Ma X, Qin G. Mol Biol Rep. 2014;41(4):2151-9. Doi: 10.1007/S11033-014-3064-2. Epub 2014 Jan 12. The Effect Of Resveratrol On The Expression Of Adipor1 In Kidneys Of Diabetic Nephropathy.
Qi My, Kai-Chen, Liu Hr, Su Yh, Yu Sq. J Ethnopharmacol. 2011 Dec 8;138(3):731-6. Doi: 10.1016/J.Jep.2011.10.015. Epub 2011 Oct 19. Protective Effect Of Icariin On The Early Stage Of Experimental Diabetic Nephropathy Induced By Streptozotocin Via ...
Wenbin Z, Guojun G. West Indian Med J. 2014 Jan;63(1):20-5. Doi: 10.7727/Wimj.2014.008. Epub 2014 Apr 8. Resveratrol Ameliorates Diabetes-Induced Renal Damage Through Regulating The Expression Of Tgf-??1, Collagen Iv And Th17/Treg-Related Cytokines...
Luo X, Deng L, Lamsal Lp, Xu W, Xiang C, Cheng L. Cell Physiol Biochem. 2015;35(1):191-200. Doi: 10.1159/000369687. Epub 2015 Jan 2. Amp-Activated Protein Kinase Alleviates Extracellular Matrix Accumulation In High Glucose-Induced Renal Fibroblast...
Xue H, Zhang Yl, Liu Gs, Wang H. J Pharmacol Exp Ther. 2005 Nov;315(2):501-9. Epub 2005 Jul 28. A New Atp-Sensitive Potassium Channel Opener Protects The Kidney From Hypertensive Damage In Spontaneously Hypertensive Rats.
He S, Xia T, Wang H, Wei L, Luo X, Li X. Acta Biomater. 2012 Jul;8(7):2659-69. Doi: 10.1016/J.Actbio.2012.03.044. Epub 2012 Apr 3. Multiple Release Of Polyplexes Of Plasmids Vegf And Bfgf From Electrospun Fibrous Scaffolds Towards Regeneration Of ...
Liu Y, Lu J, Li H, Wei J, Li X. Acta Biomater. 2015 Jan;11:114-25. Doi: 10.1016/J.Actbio.2014.10.004. Epub 2014 Oct 7. Engineering Blood Vessels Through Micropatterned Co-Culture Of Vascular Endothelial And Smooth Muscle Cells On Bilayered Electro...
Chen F, Wan H, Xia T, Guo X, Wang H, Liu Y, Li X. Eur J Pharm Biopharm. 2013 Nov;85(3 Pt A):699-710. Doi: 10.1016/J.Ejpb.2013.07.009. Epub 2013 Jul 24. Promoted Regeneration Of Mature Blood Vessels By Electrospun Fibers With Loaded Multiple Pdna-C...
Zhang Z, Wu F, Zheng F, Li H. Nephron Exp Nephrol. 2010;116(1):E11-21. Doi: 10.1159/000314669. Epub 2010 May 21. Adenovirus-Mediated Decorin Gene Transfection Has Therapeutic Effects In A Streptozocin-Induced Diabetic Rat Model.
Wen D, Huang X, Zhang M, Zhang L, Chen J, Gu Y, Hao Cm. Plos One. 2013 Dec 3;8(12):E82336. Doi: 10.1371/Journal.Pone.0082336. Ecollection 2013. Resveratrol Attenuates Diabetic Nephropathy Via Modulating Angiogenesis.
Xu L, Zheng N, He Q, Li R, Zhang K, Liang T. Phytomedicine. 2013 Oct 15;20(13):1172-9. Doi: 10.1016/J.Phymed.2013.06.008. Epub 2013 Jul 13. Puerarin, Isolated From Pueraria Lobata (Willd.), Protects Against Hepatotoxicity Via Specific Inhibition O...

FAQs

Q: Do you offer BSA-free antibodies? Keyword: Bovine serum albumin, carrier protein, conjugation
A: Yes, please contact us at support@bosterbio.com for more information about BSA-free antibodies and availability. The new BSA-free formula uses trehalose as a replacement to BSA. We have tested many alternative chemicals and found that trehalose protects the antibodies the best.
Q: Is your western blot protocol provided from the website applicable for all your antibodies? Keyword: applications, WB
A: The protocol is applicable for all our antibodies in WB, the NC Membrane(0.45μm or 0.22μm) and transfer time(70 mins or 50 mins) depends on the protein molecular weight, details can be found in included protocol.
Q: Can I conjugate markers to this antibody? Can I link custom conjugates to this antibody? Keyword: conjugation
A: The antibody is stored with BSA and cannot be conjugated with markers. Carrier free antibodies are available upon request. Please contact support@bosterbio.com
Q: What should I use for negative control?
A: Please contact us for negative control suggestions. You can also check expression databases such as genecards, uniprot etc. Due to logistic reasons, we do not sell serum or lysates that we use internally for positive or negative control.
Q: Where can I find troubleshooting information? What should I do if I have unexpected bands, high background, no signal, weak signal
A: You can find Boster's troubleshoot guides under tech support tab. Please contact us for further assistance on troubleshooting your experiment.
Q: What is the immunogen sequence of this antibody? Is this antibody polyclonal or monoclonal?
A: You can find the immunogen sequence under "Immunogen" and clonality in the product name.
Q: What is the expected band size? Why is it different than the observed band size?
A: The expected band size is predicted on the size of the protein. The actual band size may be affected by a few other factors including but not limited to:
1. Post-translational modification:phosphorylation, methylation, glycosylation etc. These modifications prevent SDS molecules from binding to the target protein and thus make the band size appear larger than expected
2. Post-translational cleavage: this can cause smaller bands and or multiple bands

3. Alternative splicing: the same gene can have alternative splicing patterns generating different size proteins, all with reactivities to the antibody.

4. Amino Acid R chain charge: SDS binds to positive charges. The different size and charge of the Amino Acid side chains can affect the amount of SDS binding and thus affect the observed band size.
5. Multimers: Multimers are usually broken up in reducing conditions. However if the interactions between the multimers are strong, the band may appear higher.,
Q: What is the suggested dilution ratio for Western Blot (WB), Immunohistochemistry (IHC) and or ELISA standards? What is the optimal pH for the sample?
A: Check the datasheet for the product for details on dilution ratios for different experiments. You can find the datasheet button on the right side of the product page.
Q: What is the protocol you used for your Western blotting (WB) and Immunohistochemistry (IHC)?
A: Check our protocols under the tech support tab.