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SKU:MA1029
Clonality:Monoclonal
Clone Number:FH-7A
Application:IHC-F, WB
Price: $200.00
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Overview

Product Name Anti-Collagen, Type III Antibody (Monoclonal, FH-7A)
Description Mouse IgG monoclonal antibody for Collagen, Type III, collagen, type III, alpha 1 (COL3A1) detection. Tested with WB, IHC-F in Human;rat. No cross reactivity with other proteins.
Cite This Product Anti-Collagen, Type III Antibody (Monoclonal, FH-7A) FH-7A (Boster Biological Technology, Pleasanton CA, USA, Catalog # MA1029)
Replacement Item This antibody may replace the following items: sc-28888|sc-8781|sc-271249|sc-8780-R|sc-514601 from Santa Cruz Biotechnology.
Host Mouse
Isotype Mouse IgG1
Validated Species Human, Rat
Application IHC-F, WB

*Our Boster Guarantee covers the use of this product in the above tested applications.

**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.

Recommended Detection Systems Boster recommends Enhanced Chemiluminescent Kit with anti-Mouse IgG (EK1001) for Western blot, and HRP Conjugated anti-Mouse IgG Super Vision Assay Kit (SV0001-1) for IHC(F).
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2017!
Immunogen Human collagen type III.
Cross Reactivity No cross reactivity with other proteins
Pack Size 100μg/vial

Properties

Clonality Monoclonal
Clone Number FH-7A
Form Lyophilized
Contents Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.
*carrier free antibody available upon request.
Concentration Add 1ml of PBS buffer will yield a concentration of 100ug/ml.
Storage At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing.
Purification Ascites
Isotype Mouse IgG1

Protein Target Info (Source: Uniprot.org)

You can check the tissue specificity below for information on selecting positive and negative control.

Gene Name Col3a1
Protein Name Collagen alpha-1(III) chain
Molecular Weight 138936 MW
Protein Function Collagen type III occurs in most soft connective tissues along with type I collagen. Involved in regulation of cortical development. Is the major ligand of ADGRG1 in the developing brain and binding to ADGRG1 inhibits neuronal migration and activates the RhoA pathway by coupling ADGRG1 to GNA13 and possibly GNA12 (By similarity). .
Sequence Similarities Belongs to the fibrillar collagen family.
Subcellular Localization Secreted, extracellular space, extracellular matrix .
Uniprot ID P13941
Alternative Names Collagen alpha-1(III) chain;Col3a1;
Research Areas |signal transduction|cytoskeleton / ecm|extracellular matrix|ecm proteins|collagen| stem cells|mesenchymal stem cells|chondrogenesis|osteogenesis|
*if product is indicated to react with multiple species, protein info is based on the human gene.

Background for Collagen alpha-1(III) chain

Collagen type III, also known as COL3A1, is assigned to chromosome 2. Ultrastructural analysis of tissues from mutant mice revealed that type III collagen is essential for normal collagen I fibrillogenesis in the cardiovascular system and other organs. Type III collagen is crucial for collagen I fibrillogenesis and for normal cardiovascular development.

Anti-Collagen, Type III Antibody (Monoclonal, FH-7A) Images

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Anti-Collagen, Type III Antibody (Monoclonal, FH-7A)
Anti-Collagen, Type III antibody (monoclonal), MA1029, Western blotting
Lane 1: HT1080 Cell Lysate
Lane 2: COLO320 Cell Lysate

Anti-Collagen, Type III Antibody (Monoclonal, FH-7A)
Anti-Collagen, Type III antibody (monoclonal), MA1029, IHC(F)
IHC(F): Mouse Kidney Tissue
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Publications

Xu Y, Zhang Ja, Xu Y, Guo Sl, Wang S, Wu D, Wang Y, Luo D, Zhou Br. Stem Cells Dev. 2015 May 1;24(9):1096-111. Doi: 10.1089/Scd.2014.0321. Epub 2015 Jan 21. Antiphotoaging Effect Of Conditioned Medium Of Dedifferentiated Adipocytes On Skin In Vivo...
Liang J, Deng X, Lin Zx, Zhao Lc, Zhang Xl. World J Gastroenterol. 2009 Sep 28;15(36):4529-37. Attenuation Of Portal Hypertension By Natural Taurine In Rats With Liver Cirrhosis.
Ge Ws, Wu Jx, Fan Jg, Wang Yj, Chen Yw. World J Gastroenterol. 2011 Sep 28;17(36):4090-8. Doi: 10.3748/Wjg.V17.I36.4090. Inhibition Of High-Mobility Group Box 1 Expression By Sirna In Rat Hepatic Stellate Cells.
Zunwen L, Shizhen Z, Dewu L, Yungui M, Pu N. Burns. 2012 May;38(3):404-13. Doi: 10.1016/J.Burns.2011.08.013. Epub 2011 Sep 16. Effect Of Tetrandrine On The Tgf-??-Induced Smad Signal Transduction Pathway In Human Hypertrophic Scar Fibroblasts In Vi...
Li X, Han J, Li L, Wang Kj, Hu Sj. Int J Cardiol. 2013 Oct 9;168(4):3340-7. Doi: 10.1016/J.Ijcard.2013.04.038. Epub 2013 May 9. Effect Of Farnesyltransferase Inhibition On Cardiac Remodeling In Spontaneously Hypertensive Rats.
Han L, Wang L, Wang Q, Li H, Zang H. Exp Ther Med. 2014 May;7(5):1337-1341. Epub 2014 Feb 20. Association Between Pelvic Organ Prolapse And Stress Urinary Incontinence With Collagen.
Zhang Y, Zhang Y, Xie Y, Gao Y, Ma J, Yuan J, Li J, Wang J, Li L, Zhang J, Chu L. J Ethnopharmacol. 2013 Jul 9;148(2):671-81. Doi: 10.1016/J.Jep.2013.05.028. Epub 2013 May 22. Multitargeted Inhibition Of Hepatic Fibrosis In Chronic Iron-Overloaded...

FAQs

Q: Is your western blot protocol provided from the website applicable for all your antibodies? Keyword: applications, WB
A: The protocol is applicable for all our antibodies in WB, the NC Membrane(0.45μm or 0.22μm) and transfer time(70 mins or 50 mins) depends on the protein molecular weight, details can be found in included protocol.
Q: Boster offers many ascites grade monoclonal antibodies that are sold at 100 microgram size. Does the 100 micrograms refer to amount of target-specific antibody? Keyword: concentration
A: MA1029 is lyophilized, and the lyophilized lump contain 100ug target-specific antibody and other protein as the agent for protein protection, the ratio is 1:20. The antibody is supplied as mouse ascites, it is not purified. We just detect it in WB.
Q: Can I conjugate markers to this antibody? Can I link custom conjugates to this antibody? Keyword: conjugation
A: The antibody is stored with BSA and cannot be conjugated with markers. Carrier free antibodies are available upon request. Please contact support@bosterbio.com
Q: Can you provide monoclonal antibodies carrier free?
A: We cannot.
Q: What are some alternative names that could be used to describe this product?
A: Some common names include but are not limited to col3a1 antibody, type iii collagen antibody