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SKU:MA1030
Pack Size:100μg/vial
Sample Size:30ug for $99, contact us for details
Clonality:Monoclonal
Clone Number:COL-94
Application:IHC
Price: $200.00
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Overview

Product Name Anti-Collagen, Type IV Antibody (Monoclonal, COL-94)
SKU/Catalog Number MA1030
Description Mouse IgG monoclonal antibody for Collagen, Type IV, collagen, type IV, alpha-1; collagen, type IV, alpha 3 (Goodpasture antigen); collagen,type IV, alpha 4; collagen, type IV, alpha 5; collagen, type IV, alpha 6 (COL4A1; COL4A3; COL4A4; COL4A5; COL4A6) detection. Tested with IHC-P, IHC-F in Human. No cross reactivity with other proteins.
Cite This Product Anti-Collagen, Type IV Antibody (Monoclonal, COL-94) COL-94 (Boster Biological Technology, Pleasanton CA, USA, Catalog # MA1030)
Replacement Item This antibody may replace the following items: sc-11360|sc-134613|sc-134614|sc-167523|sc-167524|sc-167526|sc-167528|sc-18177|sc-18178|sc-18180|sc-385017|sc-398655|sc-52317|sc-59814|sc-70866|sc-9301|sc-9302 from Santa Cruz Biotechnology.
Host Mouse
Isotype Mouse IgG1
Validated Species Human
Application IHC

*Our Boster Guarantee covers the use of this product in the above tested applications.

**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.

Recommended Detection Systems Boster recommends HRP Conjugated anti-Mouse IgG Super Vision Assay Kit (SV0001-1) for IHC(P) and IHC(F).
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2017!
Immunogen Human collagen type IV.
Cross Reactivity No cross reactivity with other proteins
Pack Size 100μg/vial

Properties

Clonality Monoclonal
Clone Number COL-94
Form Lyophilized
Contents Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.
*carrier free antibody available upon request.
Concentration Add 1ml of PBS buffer will yield a concentration of 100ug/ml.
Storage At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing.
Purification Ascites
Isotype Mouse IgG1

Protein Target Info (Source: Uniprot.org)

You can check the tissue specificity below for information on selecting positive and negative control.

Gene Name COL4A1
Protein Name Collagen alpha-1(IV) chain; Collagen alpha-3(IV) chain; Collagen alpha-4(IV) chain; Collagen alpha-5(IV) chain; Collagen alpha-6(IV) chain
Molecular Weight 160615 MW
Protein Function Type IV collagen is the major structural component of glomerular basement membranes (GBM), forming a 'chicken-wire' meshwork together with laminins, proteoglycans and entactin/nidogen.
Tissue Specificity Highly expressed in placenta. .
Sequence Similarities Belongs to the type IV collagen family.
Subcellular Localization Secreted, extracellular space, extracellular matrix, basement membrane.
Uniprot ID P02462
Alternative Names Collagen alpha-1(IV) chain;Arresten;COL4A1;
Research Areas COL4A1|COL4A3|COL4A4|COL4A5|COL4A6|
*if product is indicated to react with multiple species, protein info is based on the human gene.

Background for Collagen alpha-1(IV) chain; Collagen alpha-3(IV) chain; Collagen alpha-4(IV) chain; Collagen alpha-5(IV) chain; Collagen alpha-6(IV) chain

Collagen IV is comprised of a third chain(alpha-3) together with the 2 classical ones, alpha-1 and alpha-2. COL4A3 gene is localized to 2q36-q37 by analysis of somatic cell hybrids and by in situ hybridization. The alpha-3 chain of type IV collagen induces autoimmune Goodpasture syndrome. Mutations in the COL4A4 and COL4A3 genes cause familial benign hematuria.

Anti-Collagen, Type IV Antibody (Monoclonal, COL-94) Images

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Anti-Collagen, Type IV Antibody (Monoclonal, COL-94)
Anti-Collagen, Type IV antibody (monoclonal), MA1030, IHC(P)
IHC(P): Human Mammary Cancer Tissue
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Publications

Zhang Z, Wu F, Zheng F, Li H. Nephron Exp Nephrol. 2010;116(1):E11-21. Doi: 10.1159/000314669. Epub 2010 May 21. Adenovirus-Mediated Decorin Gene Transfection Has Therapeutic Effects In A Streptozocin-Induced Diabetic Rat Model.
Wen D, Huang X, Zhang M, Zhang L, Chen J, Gu Y, Hao Cm. Plos One. 2013 Dec 3;8(12):E82336. Doi: 10.1371/Journal.Pone.0082336. Ecollection 2013. Resveratrol Attenuates Diabetic Nephropathy Via Modulating Angiogenesis.
Xu L, Zheng N, He Q, Li R, Zhang K, Liang T. Phytomedicine. 2013 Oct 15;20(13):1172-9. Doi: 10.1016/J.Phymed.2013.06.008. Epub 2013 Jul 13. Puerarin, Isolated From Pueraria Lobata (Willd.), Protects Against Hepatotoxicity Via Specific Inhibition O...
Zhang H, Teng X, Liu Z, Zhang L, Liu Z. J Exp Clin Cancer Res. 2015 Feb 12;34:16. Doi: 10.1186/S13046-015-0132-Y. Gene Expression Profile Analyze The Molecular Mechanism Of Cxcr7 Regulating Papillary Thyroid Carcinoma Growth And Metastasis.

FAQs

Q: Is your western blot protocol provided from the website applicable for all your antibodies? Keyword: applications, WB
A: The protocol is applicable for all our antibodies in WB, the NC Membrane(0.45μm or 0.22μm) and transfer time(70 mins or 50 mins) depends on the protein molecular weight, details can be found in included protocol.
Q: Can I conjugate markers to this antibody? Can I link custom conjugates to this antibody? Keyword: conjugation
A: The antibody is stored with BSA and cannot be conjugated with markers. Carrier free antibodies are available upon request. Please contact support@bosterbio.com
Q: Can you provide monoclonal antibodies carrier free?
A: We cannot.