Data & Images
|Product Name||Anti-Collagen, Type IV Antibody (Monoclonal, COL-94)|
|Description||Mouse IgG monoclonal antibody for Collagen, Type IV, collagen, type IV, alpha-1; collagen, type IV, alpha 3 (Goodpasture antigen); collagen,type IV, alpha 4; collagen, type IV, alpha 5; collagen, type IV, alpha 6 (COL4A1; COL4A3; COL4A4; COL4A5; COL4A6) detection. Tested with IHC-P, IHC-F in Human. No cross reactivity with other proteins.|
|Cite This Product||Anti-Collagen, Type IV Antibody (Monoclonal, COL-94) COL-94 (Boster Biological Technology, Pleasanton CA, USA, Catalog # MA1030)|
|Replacement Item||This antibody may replace the following items: sc-11360|sc-134613|sc-134614|sc-167523|sc-167524|sc-167526|sc-167528|sc-18177|sc-18178|sc-18180|sc-385017|sc-398655|sc-52317|sc-59814|sc-70866|sc-9301|sc-9302 from Santa Cruz Biotechnology.|
*Our Boster Guarantee covers the use of this product in the above tested applications.
**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.
|Recommended Detection Systems||Boster recommends HRP Conjugated anti-Mouse IgG Super Vision Assay Kit (SV0001-1) for IHC(P) and IHC(F).
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2017!
|Immunogen||Human collagen type IV.|
|Cross Reactivity||No cross reactivity with other proteins|
|Contents||Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.
*carrier free antibody available upon request.
|Concentration||Add 1ml of PBS buffer will yield a concentration of 100ug/ml.|
|Storage||At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing.|
Protein Target Info (Source: Uniprot.org)
You can check the tissue specificity below for information on selecting positive and negative control.
|Protein Name||Collagen alpha-1(IV) chain; Collagen alpha-3(IV) chain; Collagen alpha-4(IV) chain; Collagen alpha-5(IV) chain; Collagen alpha-6(IV) chain|
|Molecular Weight||160615 MW|
|Protein Function||Type IV collagen is the major structural component of glomerular basement membranes (GBM), forming a 'chicken-wire' meshwork together with laminins, proteoglycans and entactin/nidogen.|
|Tissue Specificity||Highly expressed in placenta. .|
|Sequence Similarities||Belongs to the type IV collagen family.|
|Subcellular Localization||Secreted, extracellular space, extracellular matrix, basement membrane.|
|Alternative Names||Collagen alpha-1(IV) chain;Arresten;COL4A1;|
Background for Collagen alpha-1(IV) chain; Collagen alpha-3(IV) chain; Collagen alpha-4(IV) chain; Collagen alpha-5(IV) chain; Collagen alpha-6(IV) chain
Dilution Ratios/Recommended Concentrations
At Boster we strive to provide the best Anti-Collagen, Type IV Antibody (Monoclonal, COL-94) by testing all applications on non-spiked tissues and cell lines to ensure that the affinity of the antibody is enough to react to the endogenouse level of the target protein. Read more about our QC panel here.
|Recommended dilution ratios are listed below:|
Immunohistochemistry(Paraffin-embedded Section), 0.5-1μg/ml, Human, By Heat
Immunohistochemistry(Frozen Section), 0.5-1μg/ml, Human
**Boster provides high sensitivity secondary antibody kits for Western blotting and IHC. For more info see Related Products below.
Anti-Collagen, Type IV Antibody (Monoclonal, COL-94) Images
Click the images to enlarge.
IHC(P): Human Mammary Cancer Tissue