Anti-COMT Antibody Picoband™

COMT antibody

Boster Bio Anti-COMT Antibody Picoband™ catalog # A00464. Tested in ELISA, WB applications. This antibody reacts with Mouse, Rat.

Product Info Summary

SKU: A00464
Size: 100 μg/vial
Reactive Species: Mouse, Rat
Host: Rabbit
Application: ELISA, WB

Product Name

Anti-COMT Antibody Picoband™

View all COMT Antibodies

SKU/Catalog Number

A00464

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-COMT Antibody Picoband™ catalog # A00464. Tested in ELISA, WB applications. This antibody reacts with Mouse, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-COMT Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00464)

Host

Rabbit

Contents

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E. coli-derived mouse COMT recombinant protein (Position: R20-S265).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

A00464 is reactive to COMT in Mouse, Rat

Applications

A00464 is guaranteed for ELISA, WB Boster Guarantee

Observed Molecular Weight

25 kDa, 29 kDa

Calculated molecular weight

30.037kDa

Background of COMT

Catechol O-methyltransferase, also called COMT, is one of the major mammalian enzymes involved in the metabolic degradation of catecholamines. This gene is mapped to 22q11.21. Catechol-O-methyltransferase catalyzes the transfer of a methyl group from S-adenosylmethionine to catecholamines, including the neurotransmitters dopamine, epinephrine, and norepinephrine. This O-methylation results in one of the major degradative pathways of the catecholamine transmitters. In addition to its role in the metabolism of endogenous substances, COMT is important in the metabolism of catechol drugs used in the treatment of hypertension, asthma, and Parkinson disease. COMT is found in two forms in tissues, a soluble form (S-COMT) and a membrane-bound form (MB-COMT). The differences between S-COMT and MB-COMT reside within the N-termini.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml
Direct ELISA, 0.1-0.5μg/ml

Validation Images & Assay Conditions

Gene/Protein Information For COMT (Source: Uniprot.org, NCBI)

Gene Name

COMT

Full Name

Catechol O-methyltransferase

Weight

30.037kDa

Superfamily

class I-like SAM-binding methyltransferase superfamily

Alternative Names

catechol O-methyltransferase; catechol-O-methyltransferase; COMT; EC 2.1.1.6 COMT HEL-S-98n catechol-O-methyltransferase catechol O-methyltransferase|epididymis secretory sperm binding protein Li 98n|testicular tissue protein Li 42

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on COMT, check out the COMT Infographic

COMT infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for COMT: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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5 Customer Q&As for Anti-COMT Antibody Picoband™

Question

I see that the anti-COMT antibody A00464 works with ELISA, what is the protocol used to produce the result images on the product page?

A. Mangal

Verified customer

Asked: 2020-04-23

Answer

You can find protocols for ELISA on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2020-04-23

Question

I have a question about product A00464, anti-COMT antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

Verified Customer

Verified customer

Asked: 2019-11-18

Answer

We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A00464 anti-COMT antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2019-11-18

Question

Is a blocking peptide available for product anti-COMT antibody (A00464)?

Verified Customer

Verified customer

Asked: 2019-10-17

Answer

We do provide the blocking peptide for product anti-COMT antibody (A00464). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2019-10-17

Question

Will A00464 anti-COMT antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

J. Li

Verified customer

Asked: 2018-05-15

Answer

It shows on the product datasheet, A00464 anti-COMT antibody as been validated on ELISA. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2018-05-15

Question

Does anti-COMT antibody A00464 work for ELISA with placenta?

R. Kulkarni

Verified customer

Asked: 2016-02-11

Answer

According to the expression profile of placenta, COMT is highly expressed in placenta. So, it is likely that anti-COMT antibody A00464 will work for ELISA with placenta.

Boster Scientific Support

Answered: 2016-02-11

Order DetailsPrice
A00464

100μg

$370
A00464-10ug

10μg sample (liquid)

$99
A00464-Biotin

100 μg Biotin conjugated

$570
A00464-Cy3

100 μg Cy3 conjugated

$570
A00464-Dylight488

100 μg Dylight488 conjugated

$570
A00464-Dylight550

100 μg Dylight550 conjugated

$570
A00464-Dylight594

100 μg Dylight594 conjugated

$570
A00464-FITC

100 μg FITC conjugated

$570
A00464-HRP

100 μg HRP conjugated

$570
A00464-APC

100 μg APC conjugated

$670
A00464-PE

100 μg PE conjugated

$670
A00464-carrier-free

Carrier Free

$370

More conjugates/formats

Free Secondary Antibody

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A00464
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00

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