Anti-Connexin 32/GJB1 Antibody

Connexin 32/GJB1 antibody

Boster Bio Anti-Connexin 32/GJB1 Antibody catalog # PA1367. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat.

Product Info Summary

SKU: PA1367
Size: 100 μg/vial
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: IHC, WB

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Product Name

Anti-Connexin 32/GJB1 Antibody

View all Connexin 32/GJB1 Antibodies

SKU/Catalog Number

PA1367

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-Connexin 32/GJB1 Antibody catalog # PA1367. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Connexin 32/GJB1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1367)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

A synthetic peptide corresponding to a sequence in the middle region of human Connexin 32/GJB1, identical to the related mouse and rat sequences.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins

Reactive Species

PA1367 is reactive to GJB1 in Human, Mouse, Rat

Applications

PA1367 is guaranteed for IHC, WB Boster Guarantee

Observed Molecular Weight

50 kDa

Calculated molecular weight

32.025kDa

Background of Connexin 32/GJB1

Gap junction beta-1 protein (GJB1), also known as connexin 32 (Cx32) is a transmembrane protein that in humans is encoded by the GJB1 gene. This gene encodes a member of the gap junction protein family. The gap junction proteins are membrane-spanning proteins that assemble to form gap junction channels that facilitate the transfer of ions and small molecules between cells. According to sequence similarities at the nucleotide and amino acid levels, the gap junction proteins are divided into two categories, alpha and beta. Mutations in this gene cause X-linked Charcot-Marie-Tooth disease, an inherited peripheral neuropathy. Alternatively spliced transcript variants encoding the same protein have been found for this gene.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Mouse, Rat, By Heat
Western blot, 0.1-0.5μg/ml, Human, Rat, Mouse

Validation Images & Assay Conditions

Gene/Protein Information For GJB1 (Source: Uniprot.org, NCBI)

Gene Name

GJB1

Full Name

Gap junction beta-1 protein

Weight

32.025kDa

Superfamily

connexin family

Alternative Names

CMTX; CMTX1; Connexin 32; Connexin-32; CX32; CX32beta 1, 32kD (connexin 32, Charcot-Marie-Toothneuropathy, X-linked); gap junction beta-1 protein; gap junction protein, beta 1, 32kDa (connexin 32); gap junction protein, beta 1, 32kDa (connexin 32, Charcot-Marie-Toothneuropathy, X-linked); gap junction protein, beta 1, 32kDa; GJB1 GJB1 CMTX, CMTX1, CX32 gap junction protein beta 1 gap junction beta-1 protein|GAP junction 28 kDa liver protein|connexin-32|gap junction protein, beta 1, 32kDa

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on GJB1, check out the GJB1 Infographic

GJB1 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for GJB1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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4 Customer Q&As for Anti-Connexin 32/GJB1 Antibody

Question

I am interested in to test anti-Connexin 32/GJB1 antibody PA1367 on human placenta skin for research purposes, then I may be interested in using anti-Connexin 32/GJB1 antibody PA1367 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

Verified Customer

Verified customer

Asked: 2019-12-11

Answer

The products we sell, including anti-Connexin 32/GJB1 antibody PA1367, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2019-12-11

Question

I have a question about product PA1367, anti-Connexin 32/GJB1 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

Verified Customer

Verified customer

Asked: 2019-11-13

Answer

We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free PA1367 anti-Connexin 32/GJB1 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2019-11-13

Question

I have attached the WB image, lot number and protocol we used for placenta skin using anti-Connexin 32/GJB1 antibody PA1367. Please let me know if you require anything else.

P. Yang

Verified customer

Asked: 2019-04-19

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2019-04-19

Question

We are currently using anti-Connexin 32/GJB1 antibody PA1367 for mouse tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on primate tissues as well?

C. Banerjee

Verified customer

Asked: 2014-05-29

Answer

The anti-Connexin 32/GJB1 antibody (PA1367) has not been validated for cross reactivity specifically with primate tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in primate you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2014-05-29

Order DetailsPrice
PA1367

100μg

$370
PA1367-10ug

10μg sample (liquid)

$99
PA1367-Biotin

100 μg Biotin conjugated

$570
PA1367-Cy3

100 μg Cy3 conjugated

$570
PA1367-Dylight488

100 μg Dylight488 conjugated

$570
PA1367-Dylight550

100 μg Dylight550 conjugated

$570
PA1367-Dylight594

100 μg Dylight594 conjugated

$570
PA1367-FITC

100 μg FITC conjugated

$570
PA1367-HRP

100 μg HRP conjugated

$570
PA1367-APC

100 μg APC conjugated

$670
PA1367-PE

100 μg PE conjugated

$670
PA1367-carrier-free

Carrier Free

$370

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Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
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