Product Info Summary
SKU: | PA1367 |
---|---|
Size: | 100 μg/vial |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | IHC, WB |
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Product info
Product Name
Anti-Connexin 32/GJB1 Antibody
View all Connexin 32/GJB1 Antibodies
SKU/Catalog Number
PA1367
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Connexin 32/GJB1 Antibody catalog # PA1367. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Connexin 32/GJB1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1367)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence in the middle region of human Connexin 32/GJB1, identical to the related mouse and rat sequences.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PA1367 is reactive to GJB1 in Human, Mouse, Rat
Applications
PA1367 is guaranteed for IHC, WB Boster Guarantee
Observed Molecular Weight
50 kDa
Calculated molecular weight
32.025kDa
Background of Connexin 32/GJB1
Gap junction beta-1 protein (GJB1), also known as connexin 32 (Cx32) is a transmembrane protein that in humans is encoded by the GJB1 gene. This gene encodes a member of the gap junction protein family. The gap junction proteins are membrane-spanning proteins that assemble to form gap junction channels that facilitate the transfer of ions and small molecules between cells. According to sequence similarities at the nucleotide and amino acid levels, the gap junction proteins are divided into two categories, alpha and beta. Mutations in this gene cause X-linked Charcot-Marie-Tooth disease, an inherited peripheral neuropathy. Alternatively spliced transcript variants encoding the same protein have been found for this gene.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Reconsitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Mouse, Rat, By Heat
Western blot, 0.1-0.5μg/ml, Human, Rat, Mouse
Validation Images & Assay Conditions
Click image to see more details
Anti-Connexin 32/GJB1 antibody, PA1367, Western blotting
Lane 1: Rat Cardiac Muscle Tissue Lysate
Lane 2: Rat Cardiac Muscle Tissue Lysate
Lane 3: Rat Skeletal Muscle Tissue Lysate
Lane 4: Rat Brain Tissue Lysate
Lane 5: MCF-7 Cell Lysate
Lane 6: HELA Cell Lysate
Lane 7: SMMC Cell Lysate
Lane 8: JURKAT Cell Lysate
Lane 9: COLO320 Cell Lysate
Click image to see more details
Anti-Connexin 32/GJB1 antibody, PA1367, IHC(P)
IHC(P):Rat Kidney Tissue
Protein Target Info & Infographic
Gene/Protein Information For GJB1 (Source: Uniprot.org, NCBI)
Gene Name
GJB1
Full Name
Gap junction beta-1 protein
Weight
32.025kDa
Superfamily
connexin family
Alternative Names
CMTX; CMTX1; Connexin 32; Connexin-32; CX32; CX32beta 1, 32kD (connexin 32, Charcot-Marie-Toothneuropathy, X-linked); gap junction beta-1 protein; gap junction protein, beta 1, 32kDa (connexin 32); gap junction protein, beta 1, 32kDa (connexin 32, Charcot-Marie-Toothneuropathy, X-linked); gap junction protein, beta 1, 32kDa; GJB1 GJB1 CMTX, CMTX1, CX32 gap junction protein beta 1 gap junction beta-1 protein|GAP junction 28 kDa liver protein|connexin-32|gap junction protein, beta 1, 32kDa
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on GJB1, check out the GJB1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for GJB1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Connexin 32/GJB1 Antibody (PA1367)
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No publications found for PA1367
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Customer Reviews
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Customer Q&As
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4 Customer Q&As for Anti-Connexin 32/GJB1 Antibody
Question
I am interested in to test anti-Connexin 32/GJB1 antibody PA1367 on human placenta skin for research purposes, then I may be interested in using anti-Connexin 32/GJB1 antibody PA1367 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-12-11
Answer
The products we sell, including anti-Connexin 32/GJB1 antibody PA1367, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-12-11
Question
I have a question about product PA1367, anti-Connexin 32/GJB1 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-11-13
Answer
We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free PA1367 anti-Connexin 32/GJB1 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-11-13
Question
I have attached the WB image, lot number and protocol we used for placenta skin using anti-Connexin 32/GJB1 antibody PA1367. Please let me know if you require anything else.
P. Yang
Verified customer
Asked: 2019-04-19
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-04-19
Question
We are currently using anti-Connexin 32/GJB1 antibody PA1367 for mouse tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on primate tissues as well?
C. Banerjee
Verified customer
Asked: 2014-05-29
Answer
The anti-Connexin 32/GJB1 antibody (PA1367) has not been validated for cross reactivity specifically with primate tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in primate you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2014-05-29