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SKU:PA1026
Clonality:Polyclonal
Application:IHC-P, WB
Price: $200.00
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Overview

Product Name Anti-Connexin 43/GJA1 Antibody
Description Rabbit IgG polyclonal antibody for Gap junction alpha-1 protein( GJA1) detection. Tested with WB, IHC-P in Human;Mouse;Rat.
Cite This Product Anti-Connexin 43/GJA1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1026)
Replacement Item This antibody may replace the following items: sc-101660|sc-12899-R|sc-12900|sc-12900-R|sc-13558|sc-17218|sc-17218-R|sc-17220|sc-17220-R|sc-25165|sc-25165-R|sc-271837|sc-59949|sc-6560|sc-6560-R|sc-9059 from Santa Cruz Biotechnology.
Host Rabbit
Isotype N/A
Validated Species Human, Mouse, Rat
Predicted Species Chicken

*This antibody is predicted to react with the above species based on antigen sequence similarities. Our Boster Guarantee covers the use of this product with the above species.

Application IHC-P, WB

*Our Boster Guarantee covers the use of this product in the above tested applications.

**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.

Recommended Detection Systems Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P).
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2017!
Immunogen A synthetic peptide corresponding to a sequence at the C-terminus of human Connexin 43(366-382aa RASSRASSRPRPDDLEI), identical to the related rat and mouse sequences.
Cross Reactivity No cross reactivity with other proteins
Pack Size 100μg/vial

Properties

Clonality Polyclonal
Form Lyophilized
Contents Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
*carrier free antibody available upon request.
Concentration Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Storage At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing.
Purification Immunogen affinity purified.
Isotype N/A

Protein Target Info (Source: Uniprot.org)

You can check the tissue specificity below for information on selecting positive and negative control.

Gene Name GJA1
Protein Name Gap junction alpha-1 protein
Molecular Weight 43008 MW
Protein Function Gap junction protein that acts as a regulator of bladder capacity. A gap junction consists of a cluster of closely packed pairs of transmembrane channels, the connexons, through which materials of low MW diffuse from one cell to a neighboring cell. May play a critical role in the physiology of hearing by participating in the recycling of potassium to the cochlear endolymph. Negative regulator of bladder functional capacity: acts by enhancing intercellular electrical and chemical transmission, thus sensitizing bladder muscles to cholinergic neural stimuli and causing them to contract (By similarity). .
Tissue Specificity Expressed in the heart and fetal cochlea. .
Sequence Similarities Belongs to the connexin family. Alpha-type (group II) subfamily.
Subcellular Localization Cell membrane ; Multi-pass membrane protein . Cell junction, gap junction . Endoplasmic reticulum .
Uniprot ID P17302
Alternative Names Gap junction alpha-1 protein;Connexin-43;Cx43;Gap junction 43 kDa heart protein;GJA1;GJAL;
Research Areas |signal transduction|cytoskeleton / ecm|cell adhesion|gap junctions| cardiovascular|atherosclerosis|vascular inflammation|leukocyte recruitment|cell junction molecules|heart|cardiac arrhythmias|
*if product is indicated to react with multiple species, protein info is based on the human gene.

Background for Gap junction alpha-1 protein

Connexins 43(Cx43), also called GAP Junction Protein, alpha-1(GJA1). Connexin 43 is a member of the connexin gene family which abundantly expressed in the heart and liver and was mapped to 6q21-q23.2. Connexin43, the major protein of gap junctions in the heart, is targeted by several protein kinases that regulate myocardial cell-cell coupling. Mutations in the connexin43 gap-junction gene, which lead to abnormally regulated cell-cell communication, are associated with visceroatrial heterotaxia. Cx43 must also play a critical role in the physiology of hearing, presumably by participating in the recycling of potassium to the cochlear endolymph.

Anti-Connexin 43/GJA1 Antibody Images

Click the images to enlarge.

Anti-Connexin 43/GJA1 Antibody
Anti-Connexin 43/GJA1 antibody, PA1026, Western blotting
Lane 1: Rat Cardiac Muscle Tissue Lysate
Lane 2: Rat Cardiac Muscle Tissue Lysate
Lane 3: Rat Brain Tissue Lysate
Anti-Connexin 43/GJA1 Antibody
Anti-Connexin 43/GJA1 antibody, PA1026, IHC(P)
IHC(P): Rat Cardiac Muscle Tissue
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Publications

Li H, Tang M, Liang H, Li Y, Wang J, Song Y, Zheng Y, Xi J, Zhang J, Hescheler J, Zhu M. Cell Physiol Biochem. 2013;32(1):53-63. Doi: 10.1159/000350124. Epub 2013 Jul 4. Coculture Of Embryonic Ventricular Myocytes And Mouse Embryonic Stem Cell Enh...
Zhang X, Gao Y, Dong J, Wang S, Yao B, Zhang J, Hu S, Xu X, Zuo H, Wang L, Zhou H, Zhao L, Peng R. Plos One. 2014 Jul 3;9(7):E101532. Doi: 10.1371/Journal.Pone.0101532. Ecollection 2014. The Compound Chinese Medicine "Kang Fu Ling" Protects Agains...
Peng Hj, Dai Dz, Ji H, Dai Y. Eur J Pharmacol. 2010 May 25;634(1-3):101-6. Doi: 10.1016/J.Ejphar.2010.02.001. Epub 2010 Feb 16. The Separate Roles Of Endothelin Receptors Participate In Remodeling Of Matrix Metalloproteinase And Connexin 43 Of Car...

FAQs

Q: Do you offer BSA-free antibodies? Keyword: Bovine serum albumin, carrier protein, conjugation
A: Yes, please contact us at support@bosterbio.com for more information about BSA-free antibodies and availability. The new BSA-free formula uses trehalose as a replacement to BSA. We have tested many alternative chemicals and found that trehalose protects the antibodies the best.
Q: Is your western blot protocol provided from the website applicable for all your antibodies? Keyword: applications, WB
A: The protocol is applicable for all our antibodies in WB, the NC Membrane(0.45μm or 0.22μm) and transfer time(70 mins or 50 mins) depends on the protein molecular weight, details can be found in included protocol.
Q: Can I conjugate markers to this antibody? Can I link custom conjugates to this antibody? Keyword: conjugation
A: The antibody is stored with BSA and cannot be conjugated with markers. Carrier free antibodies are available upon request. Please contact support@bosterbio.com
Q: What should I use for negative control?
A: Please contact us for negative control suggestions. You can also check expression databases such as genecards, uniprot etc. Due to logistic reasons, we do not sell serum or lysates that we use internally for positive or negative control.
Q: Where can I find troubleshooting information? What should I do if I have unexpected bands, high background, no signal, weak signal
A: You can find Boster's troubleshoot guides under tech support tab. Please contact us for further assistance on troubleshooting your experiment.
Q: What is the immunogen sequence of this antibody? Is this antibody polyclonal or monoclonal?
A: You can find the immunogen sequence under "Immunogen" and clonality in the product name.
Q: What is the expected band size? Why is it different than the observed band size?
A: The expected band size is predicted on the size of the protein. The actual band size may be affected by a few other factors including but not limited to:
1. Post-translational modification:phosphorylation, methylation, glycosylation etc. These modifications prevent SDS molecules from binding to the target protein and thus make the band size appear larger than expected
2. Post-translational cleavage: this can cause smaller bands and or multiple bands

3. Alternative splicing: the same gene can have alternative splicing patterns generating different size proteins, all with reactivities to the antibody.

4. Amino Acid R chain charge: SDS binds to positive charges. The different size and charge of the Amino Acid side chains can affect the amount of SDS binding and thus affect the observed band size.
5. Multimers: Multimers are usually broken up in reducing conditions. However if the interactions between the multimers are strong, the band may appear higher.,
Q: What is the suggested dilution ratio for Western Blot (WB), Immunohistochemistry (IHC) and or ELISA standards? What is the optimal pH for the sample?
A: Check the datasheet for the product for details on dilution ratios for different experiments. You can find the datasheet button on the right side of the product page.
Q: What is the protocol you used for your Western blotting (WB) and Immunohistochemistry (IHC)?
A: Check our protocols under the tech support tab.
Q: What are some alternative names that could be used to describe this product?
A: Some common names include but are not limited to connexin 43 antibody, connexin-43 antibody, connexin43 antibody, cx43 antibody, gja1 antibody
Q: What are some alternative names that could be used to describe this product?
A: Some common names include but are not limited to connexin 43 antibody, connexin-43 antibody, connexin43 antibody, cx43 antibody, gja1 antibody