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Product Info Summary
|Reactive Species:||Human, Mouse, Rat|
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Anti-COX2/Cyclooxygenase 2/PTGS2 Antibody
Boster Bio Anti-COX2/Cyclooxygenase 2/PTGS2 Antibody catalog # PA1211. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-COX2/Cyclooxygenase 2/PTGS2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1211)
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
A synthetic peptide corresponding to a sequence at the N-terminus of human COX2 (26-41aa CQNRGVCMSVGFDQYK), different from the related rat sequence by two amino acids.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
No cross reactivity with other proteins
PA1211 is reactive to PTGS2 in Human, Mouse, Rat
PA1211 is guaranteed for IHC, WB Boster Guarantee
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.
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Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, By Heat
Western blot, 0.1-0.5μg/ml, Human, Rat, Mouse
Validation Images & Assay Conditions
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Anti-COX2/Cyclooxygenase 2 antibody, PA1211, Western blotting
Lane 1: MCF-7 Cell Lysate
Lane 2: HELA Cell Lysate
Lane 3: MM453 Cell Lysate
Lane 4: JURKAT Cell Lysate
Lane 5: COLO320 Cell Lysate
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Anti-COX2/Cyclooxygenase 2 antibody, PA1211, IHC(P)
IHC(P): Human Mammary Cancer Tissue
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Figure 3. Western blot analysis of PA1211 using anti-PA1211 antibody (PA1211).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: rat kidney tissue lysates
Lane 2: mouse kidney tissue lysates
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PA1211 antigen affinity purified polyclonal antibody (Catalog # PA1211) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PA1211 at approximately 75KD. The expected band size for PA1211 is at 69KD.
Gene/Protein Information For PTGS2 (Source: Uniprot.Org, NCBI)
Prostaglandin G/H synthase 2
prostaglandin G/H synthase family
COX2; COX-2; COX2cyclooxygenase 2b; cyclooxygenase-2; EC 1.14.99; EC 126.96.36.199; GRIPGHS; hCox-2; PGG/HS; PGH synthase 2; PGHS-2; PHS II; PHS-2; PHS-II; prostaglandin G/H synthase 2; prostaglandin G/H synthase and cyclooxygenase; Prostaglandin H2 synthase 2; prostaglandin-endoperoxide synthase 2 (prostaglandin G/H synthase andcyclooxygenase); Prostaglandin-endoperoxide synthase 2; PTGS2 PTGS2 COX-2, COX2, GRIPGHS, PGG/HS, PGHS-2, PHS-2, hCox-2 prostaglandin-endoperoxide synthase 2 prostaglandin G/H synthase 2|PGH synthase 2|PHS II|cyclooxygenase 2|cyclooxygenase 2b|prostaglandin H2 synthase 2|prostaglandin-endoperoxide synthase 2 (prostaglandin G/H synthase and cyclooxygenase)*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
For more info on PTGS2, check out the PTGS2 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for PTGS2: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]
PA1211 has been cited in 35 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Yuan L,Li Q,Bai D,Shang X,Hu F,Chen Z,An T,Chen Y,Zhang X.La2O3 Nanoparticles Induce Reproductive Toxicity Mediated by the Nrf-2/ARE Signaling Pathway in Kunming Mice.Int J Nanomedicine.2020 May 14;15:3415-3431.doi:10.2147/IJN.S230949.PMID:32523341;PMCID:PMC7236057.
PA1211 usage in article: APP:WB, SAMPLE:TESTIS TISSUE, DILUTION:1:400
Li X,Liang X,Li S,Qi X,Du N,Yang D. Effect of environmental tobacco smoke on COX-2 and SHP-2 expression in a periodontitis rat model. Oral Dis.2020 Jul 8.doi:10.1111/odi.13538. Epub ahead of print. PMID:32640491.
PA1211 usage in article: APP:IHC, SAMPLE:MAXILLA TISSUE, DILUTION:1:50
Chitosan/hyaluronic acid/plasmid-DNA nanoparticles encoding interleukin-1 receptor antagonist attenuate inflammation in synoviocytes induced by %u2026
Induced pluripotent stem cells inhibit bleomycin-induced pulmonary fibrosis in mice through suppressing TGF-%u03B21/Smad-mediated epithelial to mesenchymal %u2026
Combination of fasudil and celecoxib promotes the recovery of injured spinal cord in rats better than celecoxib or fasudil alone
Puerarin protects brain tissue against cerebral ischemia/reperfusion injury by inhibiting the inflammatory response
Effect of cyclooxygenase-2 inhibition on the development of post-traumatic stress disorder in rats
MicroRNA-101 regulates the viability and invasion of cervical cancer cells
Granulocyte Macrophage-Colony Stimulating Factor (GM-CSF) Downregulates the Expression of Protumor Factors Cyclooxygenase-2 and Inducible Nitric Oxide Synthase in a GM-CSF Receptor-Independent Manner in Cervical Cancer Cells
Odontogenic epithelial proliferation is correlated with COX-2 expression in dentigerous cyst and ameloblastoma
Anti-inflammatory Effects of Phyllanthus emblica L on Benzopyrene-Induced Precancerous Lung Lesion by Regulating the IL-1?/miR-101/Lin28B Signaling Pathway
miR-144 and targets, c-fos and cyclooxygenase-2 (COX2), modulate synthesis of PGE2 in the amnion during pregnancy and labor
Glycyrrhizic Acid Attenuates Sepsis-Induced Acute Kidney Injury by Inhibiting NF-?B Signaling Pathway
MicroRNA-27b Regulates Mitochondria Biogenesis in Myocytes
Tolerance of neurite outgrowth to Rho kinase inhibitors decreased by cyclooxygenase-2 inhibitor
Toll-Like Receptor 4 Prompts Human Breast Cancer Cells Invasiveness via Lipopolysaccharide Stimulation and Is Overexpressed in Patients with Lymph Node Metastasis
Preparation and Characterization of a Novel Aspirin Derivative with Anti-Thrombotic and Gastric Mucosal Protection Properties
Chemokine CXCL1 enhances inflammatory pain and increases NMDA receptor activity and COX-2 expression in spinal cord neurons via activation of CXCR2
Wang N, Siu F, Zhang Y. Am J Transl Res. 2017 Nov 15;9(11):4902-4913. eCollection 2017. Effect of astragaloside IV on diabetic gastric mucosa in vivo and in vitro
Li, A., Zhang, J.Y., Xiao, X., Wang, S.S., Wan, J.B., Chai, Y.S.,..., & Wang, Y.T. Hepatorenal protective effects of medicinal herbs in An-Gong-Niu-Huang Wan (AGNH) against cinnabar- and realgar-induced oxidative stress and inflammatory damage in ...
Sun, G., Yang, W., Zhang, Y., & Zhao, M. (2017). Esculentoside A ameliorates cecal ligation and puncture-induced acute kidney injury in rats. Experimental Animals. Advance online publication. doi: 10.1538/expanim.16-0102
Li L, Chen X, Lv S, Dong M, Zhang L, Tu J, Yang J, Zhang L, Song Y, Xu L, Zou J. Plos One. 2014 Nov 13;9(11):E112845. Doi: 10.1371/Journal.Pone.0112845. Ecollection 2014. Influence Of Exercise On Bone Remodeling-Related Hormones And Cytokines In O...
Tu Xk, Yang Wz, Shi Ss, Wang Ch, Zhang Gl, Ni Tr, Chen Cm, Wang R, Jia Jw, Song Qm. Neurochem Res. 2010 Aug;35(8):1147-55. Doi: 10.1007/S11064-010-0167-6. Epub 2010 Apr 13. Spatio-Temporal Distribution Of Inflammatory Reaction And Expression Of Tl...
Tu Xk, Yang Wz, Shi Ss, Chen Y, Wang Ch, Chen Cm, Chen Z. Inflammation. 2011 Oct;34(5):463-70. Doi: 10.1007/S10753-010-9254-8. Baicalin Inhibits Tlr2/4 Signaling Pathway In Rat Brain Following Permanent Cerebral Ischemia.
Wang Qs, Yang L, Cui Wy, Chen L, Jiang Yh. Plos One. 2014 Mar 5;9(3):E89149. Doi: 10.1371/Journal.Pone.0089149. Ecollection 2014. Anti-Inflammatory And Anti-Nociceptive Activities Of Methanol Extract From Aerial Part Of Phlomis Younghusbandii Muke...
Zhang X, Xu X, Xu T, Qin S. Drug Dev Res. 2014 May;75(3):195-201. Doi: 10.1002/Ddr.21170. Epub 2014 Mar 11. ??-Ecdysterone Suppresses Interleukin-1??-Induced Apoptosis And Inflammation In Rat Chondrocytes Via Inhibition Of Nf-??b Signaling Pathway.
Alsaegh Ma, Miyashita H, Zhu Sr. Pathol Oncol Res. 2015 Jan;21(1):65-71. Doi: 10.1007/S12253-014-9789-3. Epub 2014 May 15. Expression Of Human Papillomavirus Is Correlated With Ki-67 And Cox-2 Expressions In Keratocystic Odontogenic Tumor.
Wang J, Du Jr, Wang Y, Kuang X, Wang Cy. Acta Pharmacol Sin. 2010 Jul;31(7):791-7. Doi: 10.1038/Aps.2010.71. Epub 2010 Jun 28. Z-Ligustilide Attenuates Lipopolysaccharide-Induced Proinflammatory Response Via Inhibiting Nf-Kappab Pathway In Primary...
Jiang W, Gao M, Sun S, Bi A, Xin Y, Han X, Wang L, Yin Z, Luo L. Biochem Biophys Res Commun. 2012 Jun 1;422(2):344-50. Doi: 10.1016/J.Bbrc.2012.05.022. Epub 2012 May 11. Protective Effect Of L-Theanine On Carbon Tetrachloride-Induced Acute Liver I...
Liao Y, Liu P, Guo F, Zhang Zy, Zhang Z. Plos One. 2013 Jul 24;8(7):E68963. Doi: 10.1371/Journal.Pone.0068963. Print 2013. Oxidative Burst Of Circulating Neutrophils Following Traumatic Brain Injury In Human.
Duan J, Zhang Y, Han S, Chen Y, Li B, Liao M, Chen W, Deng X, Zhao J, Huang B. Int J Pharm. 2010 Nov 15;400(1-2):211-20. Doi: 10.1016/J.Ijpharm.2010.08.033. Epub 2010 Sep 8. Synthesis And In Vitro/In Vivo Anti-Cancer Evaluation Of Curcumin-Loaded ...
Xie Y, Chen S, Wang Ch, Tang Cw. Cancer Biol Ther. 2011 Jul 1;12(1):86-92. Doi: 10.4161/Cbt.12.1.15730. Epub 2011 Jul 1. Som230 Combined With Celecoxib Prolongs The Survival In Nude Mice With Hepg-2 Xenografts.
Guo J, Li F, Wu Q, Gong Q, Lu Y, Shi J. Phytomedicine. 2010 Oct;17(12):950-5. Doi: 10.1016/J.Phymed.2010.03.007. Epub 2010 Apr 9. Protective Effects Of Icariin On Brain Dysfunction Induced By Lipopolysaccharide In Rats.
Ni Yf, Kuai Jk, Lu Zf, Yang Gd, Fu Hy, Wang J, Tian F, Yan Xl, Zhao Yc, Wang Yj, Jiang T. J Surg Res. 2011 Jan;165(1):E29-35. Doi: 10.1016/J.Jss.2010.10.004. Epub 2010 Nov 10. Glycyrrhizin Treatment Is Associated With Attenuation Of Lipopolysaccha...
Liu H, Yan Zq, Li B, Yin Sy, Sun Q, Kou Jj, Ye D, Ferns K, Liu Hy, Liu Sl. J Ovarian Res. 2014 Sep 5;7:87. Doi: 10.1186/S13048-014-0087-1. Reduced Expression Of Sox7 In Ovarian Cancer: A Novel Tumor Suppressor Through The Wnt/??-Catenin Signaling ...
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