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SKU:PA1725
Pack Size:100μg/vial
Sample Size:30ug for $99, contact us for details
Clonality:Polyclonal
Application:IHC-P, IHC-F, WB
Price: $200.00
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Overview

Product Name Anti-COX2/Cyclooxygenase 2 Antibody
SKU/Catalog Number PA1725
Description Rabbit IgG polyclonal antibody for Prostaglandin G/H synthase 2(PTGS2) detection. Tested with WB, IHC-P, IHC-F in Human.
Cite This Product Anti-COX2/Cyclooxygenase 2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1725)
Replacement Item This antibody may replace the following items: sc-56182|sc-99|sc-98|sc-53396|sc-53397|sc-1314|sc-1312|sc-65226|sc-6243|sc-374087|sc-47698|sc-53394|sc-17846|sc-55476|sc-263|sc-73566|sc-81168|sc-56180|sc-65334|sc-136023|sc-71821|sc-71820|sc-71818|sc-71819|sc-71817|sc-17577|sc-17578|sc-25767|sc-1313|sc-100|sc-74573|sc-1315|sc-74574|sc-126|sc-393031|sc-6243-G|sc-56179|sc-71815|sc-98-X|sc-6243-X|sc-126-X|sc-1311-R|sc-71817-X|sc-1315-X from Santa Cruz Biotechnology.
Host Rabbit
Isotype N/A
Validated Species Human
Predicted Species Bovine

*This antibody is predicted to react with the above species based on antigen sequence similarities. Our Boster Guarantee covers the use of this product with the above species.

Application IHC-P, IHC-F, WB

*Our Boster Guarantee covers the use of this product in the above tested applications.

**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.

Recommended Detection Systems Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P) and IHC(F).
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2017!
Immunogen A synthetic peptide corresponding to a sequence at the C-terminus of human COX2(589-604aa DDINPTVLLKERSTEL).
Cross Reactivity No cross reactivity with other proteins
Pack Size 100μg/vial

Properties

Clonality Polyclonal
Form Lyophilized
Contents Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
*carrier free antibody available upon request.
Concentration Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Storage At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing.
Purification Immunogen affinity purified.
Isotype N/A

Protein Target Info (Source: Uniprot.org)

You can check the tissue specificity below for information on selecting positive and negative control.

Gene Name PTGS2
Protein Name Prostaglandin G/H synthase 2
Molecular Weight 68996 MW
Protein Function Converts arachidonate to prostaglandin H2 (PGH2), a committed step in prostanoid synthesis. Constitutively expressed in some tissues in physiological conditions, such as the endothelium, kidney and brain, and in pathological conditions, such as in cancer. PTGS2 is responsible for production of inflammatory prostaglandins. Up-regulation of PTGS2 is also associated with increased cell adhesion, phenotypic changes, resistance to apoptosis and tumor angiogenesis. In cancer cells, PTGS2 is a key step in the production of prostaglandin E2 (PGE2), which plays important roles in modulating motility, proliferation and resistance to apoptosis. .
Sequence Similarities Belongs to the prostaglandin G/H synthase family.
Subcellular Localization Microsome membrane; Peripheral membrane protein. Endoplasmic reticulum membrane; Peripheral membrane protein.
Uniprot ID P35354
Alternative Names Prostaglandin G/H synthase 2;1.14.99.1;Cyclooxygenase-2;COX-2;PHS II;Prostaglandin H2 synthase 2;PGH synthase 2;PGHS-2;Prostaglandin-endoperoxide synthase 2;PTGS2;COX2;
Research Areas |cardiovascular|blood|platelets| cardiovascular|lipids / lipoproteins|lipid metabolism|cholesterol metabolism| signal transduction|signaling pathway|lipid signaling|prostaglandins| cancer|tumor biomarkers|atherosclerosis|lipoprotein metabolism|cancer metabolism|metabolic signaling pathway|hormone biosynthesis| metabolism|pathways and processes|metabolic signaling pathways|lipid and lipoprotein metabolism|endocrine metabolism|types of disease|heart disease|
*if product is indicated to react with multiple species, protein info is based on the human gene.

Background for Prostaglandin G/H synthase 2

Cyclooxygenase(Cox) is the key enzyme in conversion of arachidonic acid to PGs, and two isoforms, Cox-1 and Cox-2, have been identified. Cox-2 gene encodes an inducible prostaglandin synthase enzyme that is overexpressed in adenocarcinomas and other tumors. Deletion of the murine Cox-2 gene in Min mice reduced the incidence of intestinal tumors, suggesting that it is required for tumorigenesis. This gene is localized to sites associated with retinal blood vessels, and plays an important role in blood vessel formation in the retina. And the glucocorticoid receptor suppression of COX-2 is also crucial for curtailing lethal immune activation, and suggest new therapeutic approaches for regulation of T-cell-mediated inflammatory diseases.

Anti-COX2/Cyclooxygenase 2 Antibody Images

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Anti-COX2/Cyclooxygenase 2 Antibody
Anti-COX2/Cyclooxygenase 2 antibody, PA1725, Western blotting
All lanes: Anti COX2/Cyclooxygenase 2 (PA1725) at 0.5ug/ml
Lane 1: Human Placenta Tissue Lysate at 50ug
Lane 2: COLO320 Whole Cell Lysate at 40ug
Lane 3: HELA Whole Cell Lysate at 40ug
Lane 4: PANC Whole Cell Lysate at 40ug
Lane 5: SKOV Whole Cell Lysate at 40ug
Predicted bind size: 69KD
Observed bind size: 69KD
Anti-COX2/Cyclooxygenase 2 Antibody
Anti-COX2/Cyclooxygenase 2 antibody, PA1725, IHC(P)
IHC(P): Human Intestinal Cancer Tissue
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Publications

Zhang, Y., Zhang, L., Yu, C.F., Du, X.Y., Liu, X.R., Liu, J.Z.,…, & Cao, B.Y. (2017). Effects of interferon tau on endometrial epithelial cells in caprine in vitro. Gene Expression Patterns. Advance online publication. doi: 10.1016/j.gep.2017.06.010
Xian-Xiang Xu, , Xiao-Hong Zhang, Yong Diao, Yu-Xiang Huang http://dx.doi.org/10.1016/j.kjms.2016.11.004 Achyranthes bidentate saponins protect rat articular chondrocytes against interleukin-1β-induced inflammation and apoptosis in vitro
Li L, Chen X, Lv S, Dong M, Zhang L, Tu J, Yang J, Zhang L, Song Y, Xu L, Zou J. Plos One. 2014 Nov 13;9(11):E112845. Doi: 10.1371/Journal.Pone.0112845. Ecollection 2014. Influence Of Exercise On Bone Remodeling-Related Hormones And Cytokines In O...
Tu Xk, Yang Wz, Shi Ss, Wang Ch, Zhang Gl, Ni Tr, Chen Cm, Wang R, Jia Jw, Song Qm. Neurochem Res. 2010 Aug;35(8):1147-55. Doi: 10.1007/S11064-010-0167-6. Epub 2010 Apr 13. Spatio-Temporal Distribution Of Inflammatory Reaction And Expression Of Tl...
Tu Xk, Yang Wz, Shi Ss, Chen Y, Wang Ch, Chen Cm, Chen Z. Inflammation. 2011 Oct;34(5):463-70. Doi: 10.1007/S10753-010-9254-8. Baicalin Inhibits Tlr2/4 Signaling Pathway In Rat Brain Following Permanent Cerebral Ischemia.
Wang Qs, Yang L, Cui Wy, Chen L, Jiang Yh. Plos One. 2014 Mar 5;9(3):E89149. Doi: 10.1371/Journal.Pone.0089149. Ecollection 2014. Anti-Inflammatory And Anti-Nociceptive Activities Of Methanol Extract From Aerial Part Of Phlomis Younghusbandii Muke...
Zhang X, Xu X, Xu T, Qin S. Drug Dev Res. 2014 May;75(3):195-201. Doi: 10.1002/Ddr.21170. Epub 2014 Mar 11. ??-Ecdysterone Suppresses Interleukin-1??-Induced Apoptosis And Inflammation In Rat Chondrocytes Via Inhibition Of Nf-??b Signaling Pathway.
Alsaegh Ma, Miyashita H, Zhu Sr. Pathol Oncol Res. 2015 Jan;21(1):65-71. Doi: 10.1007/S12253-014-9789-3. Epub 2014 May 15. Expression Of Human Papillomavirus Is Correlated With Ki-67 And Cox-2 Expressions In Keratocystic Odontogenic Tumor.
Wang J, Du Jr, Wang Y, Kuang X, Wang Cy. Acta Pharmacol Sin. 2010 Jul;31(7):791-7. Doi: 10.1038/Aps.2010.71. Epub 2010 Jun 28. Z-Ligustilide Attenuates Lipopolysaccharide-Induced Proinflammatory Response Via Inhibiting Nf-Kappab Pathway In Primary...
Jiang W, Gao M, Sun S, Bi A, Xin Y, Han X, Wang L, Yin Z, Luo L. Biochem Biophys Res Commun. 2012 Jun 1;422(2):344-50. Doi: 10.1016/J.Bbrc.2012.05.022. Epub 2012 May 11. Protective Effect Of L-Theanine On Carbon Tetrachloride-Induced Acute Liver I...
Liao Y, Liu P, Guo F, Zhang Zy, Zhang Z. Plos One. 2013 Jul 24;8(7):E68963. Doi: 10.1371/Journal.Pone.0068963. Print 2013. Oxidative Burst Of Circulating Neutrophils Following Traumatic Brain Injury In Human.
Duan J, Zhang Y, Han S, Chen Y, Li B, Liao M, Chen W, Deng X, Zhao J, Huang B. Int J Pharm. 2010 Nov 15;400(1-2):211-20. Doi: 10.1016/J.Ijpharm.2010.08.033. Epub 2010 Sep 8. Synthesis And In Vitro/In Vivo Anti-Cancer Evaluation Of Curcumin-Loaded ...
Xie Y, Chen S, Wang Ch, Tang Cw. Cancer Biol Ther. 2011 Jul 1;12(1):86-92. Doi: 10.4161/Cbt.12.1.15730. Epub 2011 Jul 1. Som230 Combined With Celecoxib Prolongs The Survival In Nude Mice With Hepg-2 Xenografts.
Guo J, Li F, Wu Q, Gong Q, Lu Y, Shi J. Phytomedicine. 2010 Oct;17(12):950-5. Doi: 10.1016/J.Phymed.2010.03.007. Epub 2010 Apr 9. Protective Effects Of Icariin On Brain Dysfunction Induced By Lipopolysaccharide In Rats.
Ni Yf, Kuai Jk, Lu Zf, Yang Gd, Fu Hy, Wang J, Tian F, Yan Xl, Zhao Yc, Wang Yj, Jiang T. J Surg Res. 2011 Jan;165(1):E29-35. Doi: 10.1016/J.Jss.2010.10.004. Epub 2010 Nov 10. Glycyrrhizin Treatment Is Associated With Attenuation Of Lipopolysaccha...
Liu H, Yan Zq, Li B, Yin Sy, Sun Q, Kou Jj, Ye D, Ferns K, Liu Hy, Liu Sl. J Ovarian Res. 2014 Sep 5;7:87. Doi: 10.1186/S13048-014-0087-1. Reduced Expression Of Sox7 In Ovarian Cancer: A Novel Tumor Suppressor Through The Wnt/??-Catenin Signaling P...

FAQs

Q: Do you offer BSA-free antibodies? Keyword: Bovine serum albumin, carrier protein, conjugation
A: Yes, please contact us at support@bosterbio.com for more information about BSA-free antibodies and availability. The new BSA-free formula uses trehalose as a replacement to BSA. We have tested many alternative chemicals and found that trehalose protects the antibodies the best.
Q: Is your western blot protocol provided from the website applicable for all your antibodies? Keyword: applications, WB
A: The protocol is applicable for all our antibodies in WB, the NC Membrane(0.45μm or 0.22μm) and transfer time(70 mins or 50 mins) depends on the protein molecular weight, details can be found in included protocol.
Q: Can I conjugate markers to this antibody? Can I link custom conjugates to this antibody? Keyword: conjugation
A: The antibody is stored with BSA and cannot be conjugated with markers. Carrier free antibodies are available upon request. Please contact support@bosterbio.com
Q: What should I use for negative control?
A: Please contact us for negative control suggestions. You can also check expression databases such as genecards, uniprot etc. Due to logistic reasons, we do not sell serum or lysates that we use internally for positive or negative control.
Q: Where can I find troubleshooting information? What should I do if I have unexpected bands, high background, no signal, weak signal
A: You can find Boster's troubleshoot guides under tech support tab. Please contact us for further assistance on troubleshooting your experiment.
Q: What is the immunogen sequence of this antibody? Is this antibody polyclonal or monoclonal?
A: You can find the immunogen sequence under "Immunogen" and clonality in the product name.
Q: What is the expected band size? Why is it different than the observed band size?
A: The expected band size is predicted on the size of the protein. The actual band size may be affected by a few other factors including but not limited to:
1. Post-translational modification:phosphorylation, methylation, glycosylation etc. These modifications prevent SDS molecules from binding to the target protein and thus make the band size appear larger than expected
2. Post-translational cleavage: this can cause smaller bands and or multiple bands

3. Alternative splicing: the same gene can have alternative splicing patterns generating different size proteins, all with reactivities to the antibody.

4. Amino Acid R chain charge: SDS binds to positive charges. The different size and charge of the Amino Acid side chains can affect the amount of SDS binding and thus affect the observed band size.
5. Multimers: Multimers are usually broken up in reducing conditions. However if the interactions between the multimers are strong, the band may appear higher.,
Q: What is the suggested dilution ratio for Western Blot (WB), Immunohistochemistry (IHC) and or ELISA standards? What is the optimal pH for the sample?
A: Check the datasheet for the product for details on dilution ratios for different experiments. You can find the datasheet button on the right side of the product page.
Q: What is the protocol you used for your Western blotting (WB) and Immunohistochemistry (IHC)?
A: Check our protocols under the tech support tab.
Q: What are some alternative names that could be used to describe this product?
A: Some common names include but are not limited to cox 2 antibody, cox-2 antibody, cox2 antibody