|Sample Size:||30ug for $99, contact us for details|
Data & Images
|Product Name||Anti-Crk p38 Picoband™ Antibody|
|Description||Rabbit IgG polyclonal antibody for Adapter molecule crk(CRK) detection. Tested with WB, IHC-P in Human;Mouse;Rat.|
|Cite This Product||Anti-Crk p38 Picoband™ Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9101)|
|Replacement Item||This antibody may replace the following items: sc-130186|sc-17989|sc-23775|sc-271260|sc-289|sc-31693|sc-31694|sc-319|sc-365092|sc-365471|sc-390132|sc-393160|sc-81234|sc-9004|sc-9005 from Santa Cruz Biotechnology.|
|Validated Species||Human, Mouse, Rat|
*Our Boster Guarantee covers the use of this product in the above tested applications.
**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.
|Recommended Detection Systems||Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P).
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2017!
|Immunogen||E.coli-derived human Crk p38 recombinant protein (Position: A2-R246). Human Crk p38 shares 99% and 100% amino acid (aa) sequences identity with mouse and rat Crk p38, respectively.|
|Cross Reactivity||No cross reactivity with other proteins|
|Contents||Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
*carrier free antibody available upon request.
|Concentration||Add 0.2ml of distilled water will yield a concentration of 500ug/ml.|
|Storage||At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing.|
|Purification||Immunogen affinity purified.|
Protein Target Info (Source: Uniprot.org)
You can check the tissue specificity below for information on selecting positive and negative control.
|Protein Name||Adapter molecule crk|
|Molecular Weight||33831 MW|
|Protein Function||The Crk-I and Crk-II forms differ in their biological activities. Crk-II has less transforming activity than Crk-I. Crk- II mediates attachment-induced MAPK8 activation, membrane ruffling and cell motility in a Rac-dependent manner. Involved in phagocytosis of apoptotic cells and cell motility via its interaction with DOCK1 and DOCK4. May regulate the EFNA5-EPHA3 signaling. .|
|Sequence Similarities||Belongs to the CRK family.|
|Subcellular Localization||Cytoplasm . Cell membrane . Translocated to the plasma membrane upon cell adhesion. .|
|Alternative Names||Adapter molecule crk;Proto-oncogene c-Crk;p38;CRK;|
|Research Areas|||signal transduction|adapters|cytoplasmic| cancer||
Background for Adapter molecule crk
Dilution Ratios/Recommended Concentrations
At Boster we strive to provide the best Anti-Crk p38 Picoband™ Antibody by testing all applications on non-spiked tissues and cell lines to ensure that the affinity of the antibody is enough to react to the endogenouse level of the target protein. Read more about our QC panel here.
|Recommended dilution ratios are listed below:|
Immunohistochemistry(Paraffin-embedded Section), 0.5-1μg/ml, Human, Mouse, Rat, By Heat|
Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat
**Boster provides high sensitivity secondary antibody kits for Western blotting and IHC. For more info see Related Products below.
Anti-Crk p38 Picoband™ Antibody Images
Click the images to enlarge.
All lanes: Anti Crk p38 (PB9101) at 0.5ug/ml
WB: Recombinant Human CRKp38 Protein 0.5ng
Predicted bind size: 38KD
Observed bind size: 38KD
All lanes: Anti Crk p38 (PB9101) at 0.5ug/ml
Lane 1: Rat Spleen Tissue Lysate at 50ug
Lane 2: Rat Thymus Tissue Lysate at 50ug
Lane 3: Rat Liver Tissue Lysate at 50ug
Lane 4: Mouse Brain Tissue Lysate at 50ug
Lane 5: HELA Whole Cell Lysate at 40ug
Lane 6: SMMC Whole Cell Lysate at 40ug
Predicted bind size: 34KD
Observed bind size: 34KD
IHC(P): Mouse Intestine Tissue
IHC(P): Rat Brain Tissue
IHC(P): Human Lung Cancer Tissue
1. Post-translational modification:phosphorylation, methylation, glycosylation etc. These modifications prevent SDS molecules from binding to the target protein and thus make the band size appear larger than expected
2. Post-translational cleavage: this can cause smaller bands and or multiple bands
3. Alternative splicing: the same gene can have alternative splicing patterns generating different size proteins, all with reactivities to the antibody.
4. Amino Acid R chain charge: SDS binds to positive charges. The different size and charge of the Amino Acid side chains can affect the amount of SDS binding and thus affect the observed band size.
5. Multimers: Multimers are usually broken up in reducing conditions. However if the interactions between the multimers are strong, the band may appear higher.,