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SKU:PB9101
Pack Size:100μg/vial
Sample Size:30ug for $99, contact us for details
Clonality:Polyclonal
Application:IHC-P, WB
Price: $240.00
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Overview

Product Name Anti-Crk p38 Picoband™ Antibody
SKU/Catalog Number PB9101
Description Rabbit IgG polyclonal antibody for Adapter molecule crk(CRK) detection. Tested with WB, IHC-P in Human;Mouse;Rat.
Cite This Product Anti-Crk p38 Picoband™ Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9101)
Replacement Item This antibody may replace the following items: sc-130186|sc-17989|sc-23775|sc-271260|sc-289|sc-31693|sc-31694|sc-319|sc-365092|sc-365471|sc-390132|sc-393160|sc-81234|sc-9004|sc-9005 from Santa Cruz Biotechnology.
Host Rabbit
Isotype N/A
Validated Species Human, Mouse, Rat
Application IHC-P, WB

*Our Boster Guarantee covers the use of this product in the above tested applications.

**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.

Recommended Detection Systems Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P).
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2017!
Immunogen E.coli-derived human Crk p38 recombinant protein (Position: A2-R246). Human Crk p38 shares 99% and 100% amino acid (aa) sequences identity with mouse and rat Crk p38, respectively.
Cross Reactivity No cross reactivity with other proteins
Pack Size 100μg/vial

Properties

Clonality Polyclonal
Form Lyophilized
Contents Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
*carrier free antibody available upon request.
Concentration Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Storage At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing.
Purification Immunogen affinity purified.
Isotype N/A

Protein Target Info (Source: Uniprot.org)

You can check the tissue specificity below for information on selecting positive and negative control.

Gene Name CRK
Protein Name Adapter molecule crk
Molecular Weight 33831 MW
Protein Function The Crk-I and Crk-II forms differ in their biological activities. Crk-II has less transforming activity than Crk-I. Crk- II mediates attachment-induced MAPK8 activation, membrane ruffling and cell motility in a Rac-dependent manner. Involved in phagocytosis of apoptotic cells and cell motility via its interaction with DOCK1 and DOCK4. May regulate the EFNA5-EPHA3 signaling. .
Sequence Similarities Belongs to the CRK family.
Subcellular Localization Cytoplasm . Cell membrane . Translocated to the plasma membrane upon cell adhesion. .
Uniprot ID P46108
Alternative Names Adapter molecule crk;Proto-oncogene c-Crk;p38;CRK;
Research Areas |signal transduction|adapters|cytoplasmic| cancer|
*if product is indicated to react with multiple species, protein info is based on the human gene.

Background for Adapter molecule crk

CRK, also known as p38 or CRKII, is a protein that in humans is encoded by the CRK gene. This gene is a member of an adapter protein family that binds to several tyrosine-phosphorylated proteins. It is mapped to 17p13.3. The CRK protein participates in the Reelin signaling cascade downstream of DAB1. The product of this gene has several SH2 and SH3 domains (src-homology domains) and is involved in several signaling pathways, recruiting cytoplasmic proteins in the vicinity of tyrosine kinase through SH2-phosphotyrosine interaction. The N-terminal SH2 domain of this protein functions as a positive regulator of transformation whereas the C-terminal SH3 domain functions as a negative regulator of transformation. Two alternative transcripts encoding different isoforms with distinct biological activity have been described.

Anti-Crk p38 Picoband™ Antibody Images

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Anti-Crk p38 Picoband™ Antibody
Anti-Crk p38 Picoband antibody, PB9101-1.jpg
All lanes: Anti Crk p38 (PB9101) at 0.5ug/ml
WB: Recombinant Human CRKp38 Protein 0.5ng
Predicted bind size: 38KD
Observed bind size: 38KD
Anti-Crk p38 Picoband™ Antibody
Anti-Crk p38 Picoband antibody, PB9101-2.jpg
All lanes: Anti Crk p38 (PB9101) at 0.5ug/ml
Lane 1: Rat Spleen Tissue Lysate at 50ug
Lane 2: Rat Thymus Tissue Lysate at 50ug
Lane 3: Rat Liver Tissue Lysate at 50ug
Lane 4: Mouse Brain Tissue Lysate at 50ug
Lane 5: HELA Whole Cell Lysate at 40ug
Lane 6: SMMC Whole Cell Lysate at 40ug
Predicted bind size: 34KD
Observed bind size: 34KD
Anti-Crk p38 Picoband™ Antibody
Anti-Crk p38 Picoband antibody, PB9101-3.JPG
IHC(P): Mouse Intestine Tissue
Anti-Crk p38 Picoband™ Antibody
Anti-Crk p38 Picoband antibody, PB9101-4.JPG
IHC(P): Rat Brain Tissue
Anti-Crk p38 Picoband™ Antibody
Anti-Crk p38 Picoband antibody, PB9101-5.JPG
IHC(P): Human Lung Cancer Tissue
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FAQs

Q: Do you offer BSA-free antibodies? Keyword: Bovine serum albumin, carrier protein, conjugation
A: Yes, please contact us at support@bosterbio.com for more information about BSA-free antibodies and availability. The new BSA-free formula uses trehalose as a replacement to BSA. We have tested many alternative chemicals and found that trehalose protects the antibodies the best.
Q: Can I conjugate markers to this antibody? Can I link custom conjugates to this antibody? Keyword: conjugation
A: The antibody is stored with BSA and cannot be conjugated with markers. Carrier free antibodies are available upon request. Please contact support@bosterbio.com
Q: What should I use for negative control?
A: Please contact us for negative control suggestions. You can also check expression databases such as genecards, uniprot etc. Due to logistic reasons, we do not sell serum or lysates that we use internally for positive or negative control.
Q: Where can I find troubleshooting information? What should I do if I have unexpected bands, high background, no signal, weak signal
A: You can find Boster's troubleshoot guides under tech support tab. Please contact us for further assistance on troubleshooting your experiment.
Q: What is the immunogen sequence of this antibody? Is this antibody polyclonal or monoclonal?
A: You can find the immunogen sequence under "Immunogen" and clonality in the product name.
Q: What is the expected band size? Why is it different than the observed band size?
A: The expected band size is predicted on the size of the protein. The actual band size may be affected by a few other factors including but not limited to:
1. Post-translational modification:phosphorylation, methylation, glycosylation etc. These modifications prevent SDS molecules from binding to the target protein and thus make the band size appear larger than expected
2. Post-translational cleavage: this can cause smaller bands and or multiple bands

3. Alternative splicing: the same gene can have alternative splicing patterns generating different size proteins, all with reactivities to the antibody.

4. Amino Acid R chain charge: SDS binds to positive charges. The different size and charge of the Amino Acid side chains can affect the amount of SDS binding and thus affect the observed band size.
5. Multimers: Multimers are usually broken up in reducing conditions. However if the interactions between the multimers are strong, the band may appear higher.,
Q: What is the suggested dilution ratio for Western Blot (WB), Immunohistochemistry (IHC) and or ELISA standards? What is the optimal pH for the sample?
A: Check the datasheet for the product for details on dilution ratios for different experiments. You can find the datasheet button on the right side of the product page.
Q: What is the protocol you used for your Western blotting (WB) and Immunohistochemistry (IHC)?
A: Check our protocols under the tech support tab.
Q: What are some alternative names that could be used to describe this product?
A: Some common names include but are not limited to p38 antibody, crk antibody