Anti-CST9L Antibody

CST9L antibody

Boster Bio Anti-CST9L Antibody (Catalog # A14479). Tested in IHC applications. This antibody reacts with Human.

Product Info Summary

SKU: A14479
Size: 100 µl
Reactive Species: Human
Host: Rabbit
Application: IHC

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Product Name

Anti-CST9L Antibody

View all CST9L Antibodies

SKU/Catalog Number

A14479

Size

100 µl

Form

Liquid

Description

Boster Bio Anti-CST9L Antibody (Catalog # A14479). Tested in IHC applications. This antibody reacts with Human.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-CST9L Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A14479)

Host

Rabbit

Contents

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Clonality

Polyclonal

Isotype

IgG

Immunogen

Synthesized peptide derived from internal of human CST9L.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

A14479 is reactive to CST9L in Human

Applications

A14479 is guaranteed for IHC Boster Guarantee

Observed Molecular Weight

Calculated molecular weight

17.276kDa

Background of CST9L

The cystatin superfamily encompasses proteins that contain multiple cystatin-like sequences. Some of the members are active cysteine protease inhibitors, while others have lost or perhaps never acquired this inhibitory activity. There are three inhibitory families in the superfamily, including the type 1 cystatins (stefins), type 2 cystatins and the kininogens. The type 2 cystatin proteins are a class of cysteine proteinase inhibitors found in a variety of human fluids and secretions. The cystatin locus on chromosome 20 contains the majority of the type 2 cystatin genes and pseudogenes. This gene is located in the cystatin locus and encodes a protein similar to mouse cystatin 9. Based on its testis-specific expression, it is likely to have a role in tissue reorganization during early testis development.

Zhang Z., Protein Sci. 13:2819-2824(2004).
Deloukas P., Nature 414:865-871(2001).
Clark H.F., Genome Res. 13:2265-2270(2003).

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Immunohistochemistry: 1:50~1:100

Validation Images & Assay Conditions

Gene/Protein Information For CST9L (Source: Uniprot.org, NCBI)

Gene Name

CST9L

Full Name

Cystatin-9-like

Weight

17.276kDa

Superfamily

cystatin family

Alternative Names

Cystatin-9-like CST9L CTES7B, bA218C14.1 cystatin 9 like cystatin-9-like|testatin|testicular tissue protein Li 45

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on CST9L, check out the CST9L Infographic

CST9L infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for CST9L: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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7 Customer Q&As for Anti-CST9L Antibody

Question

Does anti-CST9L antibody A14479 work on horse IHC with testis?

Verified Customer

Verified customer

Asked: 2020-03-20

Answer

Our lab technicians have not tested anti-CST9L antibody A14479 on horse. You can run a BLAST between horse and the immunogen sequence of anti-CST9L antibody A14479 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated horse samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in horse testis in IHC, you can get your next antibody for free.

Boster Scientific Support

Answered: 2020-03-20

Question

We are currently using anti-CST9L antibody A14479 for human tissue, and we are happy with the IHC results. The species of reactivity given in the datasheet says human. Is it likely that the antibody can work on feline tissues as well?

Verified Customer

Verified customer

Asked: 2020-02-12

Answer

The anti-CST9L antibody (A14479) has not been tested for cross reactivity specifically with feline tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in feline you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2020-02-12

Question

Is this A14479 anti-CST9L antibody reactive to the isotypes of CST9L?

Verified Customer

Verified customer

Asked: 2020-01-08

Answer

The immunogen of A14479 anti-CST9L antibody is Synthesized peptide derived from internal of human CST9L. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2020-01-08

Question

Would A14479 anti-CST9L antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

Verified Customer

Verified customer

Asked: 2019-09-26

Answer

It shows on the product datasheet, A14479 anti-CST9L antibody as been validated on IHC. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2019-09-26

Question

My question regarding product A14479, anti-CST9L antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

Verified Customer

Verified customer

Asked: 2019-08-13

Answer

We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A14479 anti-CST9L antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2019-08-13

Question

I am interested in to test anti-CST9L antibody A14479 on human brain for research purposes, then I may be interested in using anti-CST9L antibody A14479 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

Verified Customer

Verified customer

Asked: 2017-07-27

Answer

The products we sell, including anti-CST9L antibody A14479, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2017-07-27

Question

We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for brain using anti-CST9L antibody A14479. Let me know if you need anything else.

J. Banerjee

Verified customer

Asked: 2015-11-10

Answer

We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2015-11-10

Order DetailsPrice
A14479-100uL

100uL

$360

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