Product Info Summary
SKU: | M00132 |
---|---|
Size: | 100 μl |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | Flow Cytometry, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-CTCF Rabbit Monoclonal Antibody
SKU/Catalog Number
M00132
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-CTCF Rabbit Monoclonal Antibody catalog # M00132. Tested in WB, IHC, ICC/IF, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-CTCF Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00132)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
AAAE-3
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human CTCF
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
Detects ~20kDa. Does not cross-react with αB-crystallin, βL-crystallin, ΒH- crystallin, γ-crystallin, HSP25, HSP27 or HSP47 proteins.
Reactive Species
M00132 is reactive to CTCF in Human, Mouse, Rat
Applications
M00132 is guaranteed for Flow Cytometry, IF, IHC, ICC, WB Boster Guarantee
Observed Molecular Weight
90 kDa
Calculated molecular weight
82.785kDa
Background of CTCF
Phosphoinositide-3-kinase (PI3K) that phosphorylates PtdIns (Phosphatidylinositol), PtdIns4P (Phosphatidylinositol 4-phosphate) and PtdIns (4,5) P2 (Phosphatidylinositol 4,5-bisphosphate) to generate phosphatidylinositol 3,4,5-trisphosphate (PIP3) . PIP3 plays a key role by recruiting PH domain-containing proteins to the membrane, including AKT1 and PDPK1, activating signaling cascades involved in cell growth, survival, proliferation, motility and morphology. Participates in cellular signaling in response to various growth factors.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Innovating Scientists Reward
If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.
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Assay dilution & Images
Reconsitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:100-1:500
ICC/IF 1:50-1:200
FC 1:50
Validation Images & Assay Conditions
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Western blot analysis of CTCF expression in (1) HeLa cell lysate; (2) Mouse brain lysate.
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Immunofluorescent analysis using the Antibody at 1:500 dilution.
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Immunofluorescent analysis using the Antibody at 1:50 dilution.
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Immunofluorescent analysis using the Antibody at 1:50 dilution.
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Immunofluorescent analysis using the Antibody at 1:50 dilution.
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Immunofluorescent analysis using the Antibody at 1:150 dilution.
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Immunofluorescent analysis using the Antibody at 1:150 dilution.
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Immunofluorescent analysis using the Antibody at 1:500 dilution.
Protein Target Info & Infographic
Gene/Protein Information For CTCF (Source: Uniprot.org, NCBI)
Gene Name
CTCF
Full Name
Transcriptional repressor CTCF
Weight
82.785kDa
Superfamily
CTCF zinc-finger protein family
Alternative Names
11 zinc finger transcriptional repressor; 11-zinc finger protein; 11-Zinc Finger Protein2; CCCTC-binding factor (zinc finger protein); CCCTC-binding Factor; CTCF; CTCFL paralog; transcriptional repressor CTCF CTCF CFAP108, FAP108, MRD21 CCCTC-binding factor transcriptional repressor CTCF|11 zinc finger transcriptional repressor|11-zinc finger protein|CCCTC-binding factor (zinc finger protein)|CTCFL paralog
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CTCF, check out the CTCF Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CTCF: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-CTCF Rabbit Monoclonal Antibody (M00132)
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No publications found for M00132
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Customer Reviews
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Customer Q&As
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5 Customer Q&As for Anti-CTCF Rabbit Monoclonal Antibody
Question
My colleagues were happy with the WB result of your anti-CTCF Rabbit Monoclonal antibody. However we have been able to see positive staining in leukemic t-cell nucleus using this antibody. Is that expected? Could you tell me where is CTCF supposed to be expressed?
Verified Customer
Verified customer
Asked: 2020-01-21
Answer
From literature, leukemic t-cell does express CTCF. Generally CTCF expresses in nucleus, nucleoplasm. Regarding which tissues have CTCF expression, here are a few articles citing expression in various tissues:
Brain, Pubmed ID: 15616553
Cervix carcinoma, Pubmed ID: 18669648, 20068231
Leukemic T-cell, Pubmed ID: 19690332
Placenta, Pubmed ID: 15489334
Boster Scientific Support
Answered: 2020-01-21
Question
We have been able to see staining in rat placenta. Any tips? Is anti-CTCF Rabbit Monoclonal antibody supposed to stain placenta positively?
Verified Customer
Verified customer
Asked: 2017-12-20
Answer
From what I have seen in literature placenta does express CTCF. From what I have seen in Uniprot.org, CTCF is expressed in amniotic fluid, brain, placenta, cervix carcinoma, leukemic t-cell, among other tissues. Regarding which tissues have CTCF expression, here are a few articles citing expression in various tissues:
Brain, Pubmed ID: 15616553
Cervix carcinoma, Pubmed ID: 18669648, 20068231
Leukemic T-cell, Pubmed ID: 19690332
Placenta, Pubmed ID: 15489334
Boster Scientific Support
Answered: 2017-12-20
Question
My question regards using your anti-CTCF Rabbit Monoclonal antibody for centromeric region studies. Has this antibody been tested with western blotting on mouse brain? We would like to see some validation images before ordering.
Verified Customer
Verified customer
Asked: 2017-06-01
Answer
Thanks for your inquiry. This M00132 anti-CTCF Rabbit Monoclonal antibody is tested on hela cell lysate, mouse brain. It is guaranteed to work for IF, WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2017-06-01
Question
We bought anti-CTCF Rabbit Monoclonal antibody for WB on leukemic t-cell in a previous project. I am using human, and I plan to use the antibody for IF next. My lab would like examining leukemic t-cell as well as brain in our next experiment. Could you please give me some suggestion on which antibody would work the best for IF?
M. Krishna
Verified customer
Asked: 2017-04-17
Answer
I looked at the website and datasheets of our anti-CTCF Rabbit Monoclonal antibody and it seems that M00132 has been tested on human in both WB and IF. Thus M00132 should work for your application. Our Boster satisfaction guarantee will cover this product for IF in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for IF detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2017-04-17
Question
We are currently using anti-CTCF Rabbit Monoclonal antibody M00132 for human tissue, and we are content with the IF results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on feline tissues as well?
M. Baker
Verified customer
Asked: 2016-09-05
Answer
The anti-CTCF Rabbit Monoclonal antibody (M00132) has not been tested for cross reactivity specifically with feline tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in feline you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2016-09-05