Anti-Ctip1 Monoclonal Antibody

Ctip1 antibody

Boster Bio Anti-Ctip1 Monoclonal Antibody catalog # M00741. Tested in WB, IHC, ICC/IF, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.

Product Info Summary

SKU: M00741
Size: 100 μl
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: Flow Cytometry, IF, IHC, ICC, WB

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Product Name

Anti-Ctip1 Monoclonal Antibody

View all Ctip1 Antibodies

SKU/Catalog Number

M00741

Size

100 μl

Form

Liquid

Description

Boster Bio Anti-Ctip1 Monoclonal Antibody catalog # M00741. Tested in WB, IHC, ICC/IF, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Ctip1 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00741)

Host

Rabbit

Contents

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.

Clonality

Monoclonal

Clone Number

ADFD-2

Isotype

Rabbit IgG

Immunogen

A synthesized peptide derived from human Ctip1 Functions as a myeloid and B-cell proto-oncogene. May play important roles in leukemogenesis and hematopoiesis. An essential factor in lymphopoiesis, is required for B-cell formation in fetal liver. May function as a modulator of the transcriptional repression activity of ARP1.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

Detects ~20kDa. Does not cross-react with αB-crystallin, βL-crystallin, ΒH- crystallin, γ-crystallin, HSP25, HSP27 or HSP47 proteins.

Reactive Species

M00741 is reactive to Bcl11a in Human, Mouse, Rat

Applications

M00741 is guaranteed for Flow Cytometry, IF, IHC, ICC, WB Boster Guarantee

Observed Molecular Weight

133 kDa

Calculated molecular weight

83.855kDa

Background of Ctip1

Dynamin-related GTPase required for mitochondrial fusion and regulation of apoptosis. May form a diffusion barrier for proteins stored in mitochondrial cristae. Proteolytic processing in response to intrinsic apoptotic signals may lead to disassembly of OPA1 oligomers and release of the caspase activator cytochrome C (CYCS) into the mitochondrial intermembrane space.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB 1:1000-1:5000
IHC 1:50-1:200
ICC/IF 1:50-1:200
FC 1:100

Validation Images & Assay Conditions

Gene/Protein Information For Bcl11a (Source: Uniprot.org, NCBI)

Gene Name

Bcl11a

Full Name

B-cell lymphoma/leukemia 11A

Weight

83.855kDa

Alternative Names

B-cell CLL/lymphoma 11A (zinc finger protein) isoform 2; B-cell CLL/lymphoma 11A (zinc finger protein); B-cell CLL/lymphoma 11A; B-cell lymphoma/leukemia 11A; BCL11A B-cell CLL/lymphoma 11A (zinc finger protein) isoform 1; BCL-11A; BCL11A-S; BCL11A-XL; C2H2-type zinc finger protein; COUP-TF-interacting protein 1; CTIP1ecotropic viral integration site 9; ecotropic viral integration site 9 homolog; Ecotropic viral integration site 9 protein homolog; EVI-9; EVI9FLJ34997; HBFQTL5; KIAA1809BCL11A-L; Zinc finger protein 856; ZNF856FLJ10173 Bcl11a|2810047E18Rik, BCL-11A, CTI, Ctip1, D930021L15Rik, Ev, Evi, Evi9, Evi9a, Evi9b, Evi9c, mKIAA1809|B cell CLL/lymphoma 11A (zinc finger protein)|B-cell lymphoma/leukemia 11A|B-cell CLL/lymphoma 11A|COUP-TF interacting protein 1|EVI-9|ecotropic viral integration site 9 protein|myeloid leukemia

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on Bcl11a, check out the Bcl11a Infographic

Bcl11a infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for Bcl11a: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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7 Customer Q&As for Anti-Ctip1 Monoclonal Antibody

Question

I was wanting to use your anti-Ctip1 Monoclonal antibody for WB for rat lymph on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for rat lymph identification?

Verified Customer

Verified customer

Asked: 2020-02-10

Answer

You can see on the product datasheet, M00741 anti-Ctip1 Monoclonal antibody has been validated for IF, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in rat lymph in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2020-02-10

Question

I appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for lymph using anti-Ctip1 Monoclonal antibody M00741. Let me know if you need anything else.

Verified Customer

Verified customer

Asked: 2019-12-04

Answer

Thank you for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2019-12-04

Question

I see that the anti-Ctip1 Monoclonal antibody M00741 works with WB, what is the protocol used to produce the result images on the product page?

Verified Customer

Verified customer

Asked: 2019-12-02

Answer

You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2019-12-02

Question

See below the WB image, lot number and protocol we used for lymph using anti-Ctip1 Monoclonal antibody M00741. Please let me know if you require anything else.

Verified Customer

Verified customer

Asked: 2019-08-26

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2019-08-26

Question

I have a question about product M00741, anti-Ctip1 Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

Verified Customer

Verified customer

Asked: 2019-07-24

Answer

We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M00741 anti-Ctip1 Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2019-07-24

Question

We are currently using anti-Ctip1 Monoclonal antibody M00741 for rat tissue, and we are content with the IF results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on zebrafish tissues as well?

Verified Customer

Verified customer

Asked: 2017-05-24

Answer

The anti-Ctip1 Monoclonal antibody (M00741) has not been validated for cross reactivity specifically with zebrafish tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in zebrafish you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2017-05-24

Question

Does M00741 anti-Ctip1 Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

G. Li

Verified customer

Asked: 2017-01-27

Answer

You can see on the product datasheet, M00741 anti-Ctip1 Monoclonal antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2017-01-27

Order DetailsPrice
M00741

100μL

$370
M00741-30ul

30μL sample

$99
M00741-carrier-free

Carrier Free

$370

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*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
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