|Application:||IHC-P, IHC-F, WB|
Data & Images
|Product Name||Anti-CTNNA1 Picoband™ Antibody|
|Description||Rabbit IgG polyclonal antibody for Catenin alpha-1(CTNNA1) detection. Tested with WB, IHC-P, IHC-F in Human;Mouse;Rat.|
|Cite This Product||Anti-CTNNA1 Picoband™ Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9137)|
|Replacement Item||This antibody may replace the following items: sc-83177|sc-83176|sc-382220 from Santa Cruz Biotechnology.|
|Validated Species||Human, Mouse, Rat|
|Application||IHC-P, IHC-F, WB
*Our Boster Guarantee covers the use of this product in the above tested applications.
**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.
|Recommended Detection Systems||Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P) and IHC(F).
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2017!
|Immunogen||E.coli-derived human CTNNA1 recombinant protein (Position: D143-D292). Human CTNNA1 shares 98% amino acid (aa) sequence identity with mouse CTNNA1.|
|Cross Reactivity||No cross reactivity with other proteins|
|Contents||Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
*carrier free antibody available upon request.
|Concentration||Add 0.2ml of distilled water will yield a concentration of 500ug/ml.|
|Storage||At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing.|
|Purification||Immunogen affinity purified.|
Protein Target Info (Source: Uniprot.org)
You can check the tissue specificity below for information on selecting positive and negative control.
|Protein Name||Catenin alpha-1|
|Molecular Weight||100071 MW|
|Protein Function||Associates with the cytoplasmic domain of a variety of cadherins. The association of catenins to cadherins produces a complex which is linked to the actin filament network, and which seems to be of primary importance for cadherins cell-adhesion properties. Can associate with both E- and N-cadherins. Originally believed to be a stable component of E-cadherin/catenin adhesion complexes and to mediate the linkage of cadherins to the actin cytoskeleton at adherens junctions. In contrast, cortical actin was found to be much more dynamic than E-cadherin/catenin complexes and CTNNA1 was shown not to bind to F-actin when assembled in the complex suggesting a different linkage between actin and adherens junctions components. The homodimeric form may regulate actin filament assembly and inhibit actin branching by competing with the Arp2/3 complex for binding to actin filaments. May play a crucial role in cell differentiation.|
|Tissue Specificity||Expressed ubiquitously in normal tissues.|
|Sequence Similarities||Belongs to the vinculin/alpha-catenin family.|
|Subcellular Localization||Isoform 1: Cytoplasm, cytoskeleton. Cell junction, adherens junction. Cell membrane; Peripheral membrane protein; Cytoplasmic side. Cell junction. Found at cell-cell boundaries and probably at cell-matrix boundaries.|
|Alternative Names||Catenin alpha-1;Alpha E-catenin;Cadherin-associated protein;Renal carcinoma antigen NY-REN-13;CTNNA1;|
Background for Catenin alpha-1
Dilution Ratios/Recommended Concentrations
At Boster we strive to provide the best Anti-CTNNA1 Picoband™ Antibody by testing all applications on non-spiked tissues and cell lines to ensure that the affinity of the antibody is enough to react to the endogenouse level of the target protein. Read more about our QC panel here.
|Recommended dilution ratios are listed below:|
Immunohistochemistry(Frozen Section), 0.5-1μg/ml, Rat, -|
Immunohistochemistry(Paraffin-embedded Section), 0.5-1μg/ml, Human, Mouse, Rat, By Heat
Western blot, 0.1-0.5μg/ml, Human, Rat
**Boster provides high sensitivity secondary antibody kits for Western blotting and IHC. For more info see Related Products below.
Anti-CTNNA1 Picoband™ Antibody Images
Click the images to enlarge.
All lanes: Anti CTNNA1 (PB9137) at 0.5ug/ml
WB: Human Recombinant CTNNA1 Protein 0.5ng
Predicted bind size: 47KD
Observed bind size: 47KD
All lanes: Anti CTNNA1 (PB9137) at 0.5ug/ml
Lane 1: Rat Liver Tissue Lysate at 50ug
Lane 2: Rat Lung Tissue Lysate at 50ug
Lane 3: Rat Cardiac Muscle Tissue Lysate at 50ug
Lane 4: NIH3T3 Whole Cell Lysate at 40ug
Lane 5: PC-12 Whole Cell Lysate at 40ug
Lane 6: HEPG2 Whole Cell Lysate at 40ug
Lane 7: HELA Whole Cell Lysate at 40ug
Lane 8: MCF-7 Whole Cell Lysate at 40ug
Lane 9: HEPA Whole Cell Lysate at 40ug
Predicted bind size: 100KD
Observed bind size: 100KD
IHC(P): Rat Intestine Tissue
IHC(P): Human Mammary Tissue
IHC(P): Mouse Intestine Tissue
1. Post-translational modification:phosphorylation, methylation, glycosylation etc. These modifications prevent SDS molecules from binding to the target protein and thus make the band size appear larger than expected
2. Post-translational cleavage: this can cause smaller bands and or multiple bands
3. Alternative splicing: the same gene can have alternative splicing patterns generating different size proteins, all with reactivities to the antibody.
4. Amino Acid R chain charge: SDS binds to positive charges. The different size and charge of the Amino Acid side chains can affect the amount of SDS binding and thus affect the observed band size.
5. Multimers: Multimers are usually broken up in reducing conditions. However if the interactions between the multimers are strong, the band may appear higher.,