|Sample Size:||30ug for $99, contact us for details|
Data & Images
|Product Name||Anti-CUEDC2 Antibody|
|Description||Rabbit IgG polyclonal antibody for CUE domain-containing protein 2(CUEDC2) detection. Tested with WB, IHC-P in Human;Mouse;Rat.|
|Cite This Product||Anti-CUEDC2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1962)|
|Replacement Item||This antibody may replace the following items: sc-242520 from Santa Cruz Biotechnology.|
|Validated Species||Human, Mouse, Rat|
*This antibody is predicted to react with the above species based on antigen sequence similarities. Our Boster Guarantee covers the use of this product with the above species.
*Our Boster Guarantee covers the use of this product in the above tested applications.
**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.
|Recommended Detection Systems||Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P).
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2017!
|Immunogen||A synthetic peptide corresponding to a sequence at the C-terminus of human CUEDC2(267-287aa EAEEMKATYINLKPARKYRFH), identical to the related mouse sequence, and different from the related rat sequence by one amino acid.|
|Cross Reactivity||No cross reactivity with other proteins|
|Contents||Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
*carrier free antibody available upon request.
|Concentration||Add 0.2ml of distilled water will yield a concentration of 500ug/ml.|
|Storage||At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing.|
|Purification||Immunogen affinity purified.|
Protein Target Info (Source: Uniprot.org)
You can check the tissue specificity below for information on selecting positive and negative control.
|Protein Name||CUE domain-containing protein 2|
|Molecular Weight||32009 MW|
|Protein Function||Down-regulates ESR1 protein levels through the ubiquitination-proteasome pathway, regardless of the presence of 17 beta-estradiol. Also involved in 17 beta-estradiol-induced ESR1 degradation. Controls PGR protein levels through a similar mechanism. .|
|Tissue Specificity||Significantly up-regulated in breast tumor tissues compared with matched adjacent normal tissues (at protein level). Levels inversely correlate with ESR1 in breast cancers and are lower in low-grade tumors compared to high-grade tumors.|
|Sequence Similarities||Belongs to the CUEDC2 family.|
|Subcellular Localization||Cytoplasm . Nucleus .|
|Alternative Names||CUE domain-containing protein 2;CUEDC2;C10orf66;HOYS6;|
|Research Areas|||epigenetics and nuclear signaling|chromatin modifying enzymes|ubiquitylation| signal transduction|protein trafficking|nuclear import / export||
Background for CUE domain-containing protein 2
Dilution Ratios/Recommended Concentrations
At Boster we strive to provide the best Anti-CUEDC2 Antibody by testing all applications on non-spiked tissues and cell lines to ensure that the affinity of the antibody is enough to react to the endogenouse level of the target protein. Read more about our QC panel here.
|Recommended dilution ratios are listed below:|
Immunohistochemistry(Paraffin-embedded Section), 0.5-1μg/ml, Human, Mouse, Rat, By Heat|
Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat
**Boster provides high sensitivity secondary antibody kits for Western blotting and IHC. For more info see Related Products below.
Anti-CUEDC2 Antibody Images
Click the images to enlarge.
Recombinant Protein Detection Source: E.coli derived -recombinant human CuEDC2, 28.9KD (162aa tag+ N197-H287)
Lane 1: Recombinant Human CuEDC2 Protein 10ng
Lane 2: Recombinant Human CuEDC2 Protein 5ng
Lane 3: Recombinant Human CuEDC2 Protein 2.5ng
Lane 4: Recombinant Human CuEDC2 Protein 1.25ng
IHC(P): Human Thyroid Cancer Tissue
1. Post-translational modification:phosphorylation, methylation, glycosylation etc. These modifications prevent SDS molecules from binding to the target protein and thus make the band size appear larger than expected
2. Post-translational cleavage: this can cause smaller bands and or multiple bands
3. Alternative splicing: the same gene can have alternative splicing patterns generating different size proteins, all with reactivities to the antibody.
4. Amino Acid R chain charge: SDS binds to positive charges. The different size and charge of the Amino Acid side chains can affect the amount of SDS binding and thus affect the observed band size.
5. Multimers: Multimers are usually broken up in reducing conditions. However if the interactions between the multimers are strong, the band may appear higher.,