Anti-Cullin 4B/CUL4B Antibody

CUL4B antibody

Boster Bio Anti-Cullin 4B/CUL4B Antibody catalog # PA1233. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat, Zebrafish.

Product Info Summary

SKU: PA1233
Size: 100 μg/vial
Reactive Species: Human, Mouse, Rat, Zebrafish
Host: Rabbit
Application: IHC, WB

Product Name

Anti-Cullin 4B/CUL4B Antibody

View all CUL4B Antibodies

SKU/Catalog Number

PA1233

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-Cullin 4B/CUL4B Antibody catalog # PA1233. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat, Zebrafish.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Cullin 4B/CUL4B Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1233)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of human Cullin 4B, identical to the related rat and mouse sequences.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins

Reactive Species

PA1233 is reactive to CUL4B in Human, Mouse, Rat, Zebrafish

Applications

PA1233 is guaranteed for IHC, WB Boster Guarantee

Observed Molecular Weight

104 kDa

Calculated molecular weight

103.982kDa

Background of CUL4B

Cullin 4B/CUL4B encodes a scaffold protein that organizes a cullin-RING (really interesting new gene) ubiquitin ligase (E3) complex in ubiquitylation. The CUL4 gene encodes a protein of 913 amino acids. The cullin domain is located between amino acid residues 217 and 815 and is characterized a by C-terminal globular domain (cullin homology domain) and a series of N-terminal repeats (cullin repeats). Ohtake et al. (2007) characterize a fat-soluble ligand-dependent ubiquitin ligase complex in human cell lines, in which dioxin receptor (AhR) is integrated as a component of a novel cullin 4B ubiquitin ligase complex, CUL4B (AhR). Complex assembly and ubiquitin ligase activity of CUL4B (AhR) in vitro and in vivo are dependent on the AhR ligand. In the CUL4B (AhR) complex, ligand-activated AhR acts as a substrate-specific adaptor component that targets sex steroid receptors for degradation. Their findings uncover a function for AhR as an atypical component of the ubiquitin ligase complex and demonstrate a non-genomic signalling pathway in which fat-soluble ligands regulate target-protein-selective degradation through a ubiquitin ligase complex.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Zebrafish, By Heat
Western blot, 0.1-0.5μg/ml, Human, Rat, Zebrafish, Mouse

Validation Images & Assay Conditions

Gene/Protein Information For CUL4B (Source: Uniprot.org, NCBI)

Gene Name

CUL4B

Full Name

Cullin-4B

Weight

103.982kDa

Superfamily

cullin family

Alternative Names

CUL-4B; cullin 4B; cullin-4B; DKFZp686F1470; KIAA0695; MRXHF2; MRXS15; MRXSC; SFM2 CUL4B CUL-4B, MRXHF2, MRXS15, MRXSC, SFM2 cullin 4B cullin-4B

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on CUL4B, check out the CUL4B Infographic

CUL4B infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for CUL4B: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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3 Customer Q&As for Anti-Cullin 4B/CUL4B Antibody

Question

Is this PA1233 anti-Cullin 4B/CUL4B antibody reactive to the isotypes of CUL4B?

Verified Customer

Verified customer

Asked: 2020-04-27

Answer

The immunogen of PA1233 anti-Cullin 4B/CUL4B antibody is A synthetic peptide corresponding to a sequence at the C-terminus of human Cullin 4B(895-913aa DRDYMERDKENPNQYNYIA), identical to the related rat and mouse sequences. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2020-04-27

Question

We are currently using anti-Cullin 4B/CUL4B antibody PA1233 for mouse tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human, mouse, rat, zebrafish. Is it true that the antibody can work on goat tissues as well?

J. Huang

Verified customer

Asked: 2019-04-29

Answer

The anti-Cullin 4B/CUL4B antibody (PA1233) has not been tested for cross reactivity specifically with goat tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in goat you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2019-04-29

Question

Is there a BSA free version of anti-Cullin 4B/CUL4B antibody PA1233 available?

D. Krishna

Verified customer

Asked: 2013-11-28

Answer

I appreciate your recent telephone inquiry. I can confirm that some lots of this anti-Cullin 4B/CUL4B antibody PA1233 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2013-11-28

Order DetailsPrice
PA1233

100μg

$370
PA1233-10ug

10μg sample (liquid)

$99
PA1233-Biotin

100 μg Biotin conjugated

$570
PA1233-Cy3

100 μg Cy3 conjugated

$570
PA1233-Dylight488

100 μg Dylight488 conjugated

$570
PA1233-Dylight550

100 μg Dylight550 conjugated

$570
PA1233-Dylight594

100 μg Dylight594 conjugated

$570
PA1233-FITC

100 μg FITC conjugated

$570
PA1233-HRP

100 μg HRP conjugated

$570
PA1233-APC

100 μg APC conjugated

$670
PA1233-PE

100 μg PE conjugated

$670
PA1233-carrier-free

Carrier Free

$370

More conjugates/formats

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PA1233
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00

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