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SKU:PB9079
Clonality:Polyclonal
Application:IHC-P, WB
Price: $240.00
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Overview

Product Name Anti-CXCR3 Picoband™ Antibody
Description Rabbit IgG polyclonal antibody for C-X-C chemokine receptor type 3(CXCR3) detection. Tested with WB, IHC-P in Human;Rat.
Cite This Product Anti-CXCR3 Picoband™ Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9079)
Replacement Item This antibody may replace the following items: sc-133087|sc-133121|sc-137140|sc-13951|sc-6226|sc-9900|sc-9901 from Santa Cruz Biotechnology.
Host Rabbit
Isotype N/A
Validated Species Human, Rat
Application IHC-P, WB

*Our Boster Guarantee covers the use of this product in the above tested applications.

**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.

Recommended Detection Systems Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P).
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2017!
Immunogen E.coli-derived human CXCR3 recombinant protein (Position: M1-L368). Human CXCR3 shares 86% amino acid (aa) sequence identity with both mouse and rat CXCR3.
Cross Reactivity No cross reactivity with other proteins
Pack Size 100μg/vial

Properties

Clonality Polyclonal
Form Lyophilized
Contents Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
*carrier free antibody available upon request.
Concentration Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Storage At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing.
Purification Immunogen affinity purified.
Isotype N/A

Protein Target Info (Source: Uniprot.org)

You can check the tissue specificity below for information on selecting positive and negative control.

Gene Name CXCR3
Protein Name C-X-C chemokine receptor type 3
Molecular Weight 40660 MW
Protein Function Isoform 1: Receptor for the C-X-C chemokine CXCL9, CXCL10 and CXCL11 and mediates the proliferation, survival and angiogenic activity of human mesangial cells (HMC) through a heterotrimeric G-protein signaling pathway (PubMed:12782716). Binds to CCL21. Probably promotes cell chemotaxis response. .
Tissue Specificity Isoform 1 and isoform 2 are mainly expressed in heart, kidney, liver and skeletal muscle. Isoform 1 is also expressed in placenta. Isoform 2 is expressed in endothelial cells. Expressed in T-cells (at protein level). .
Sequence Similarities Belongs to the G-protein coupled receptor 1 family.
Subcellular Localization Isoform 1: Cell membrane ; Multi-pass membrane protein .
Uniprot ID P49682
Alternative Names C-X-C chemokine receptor type 3;CXC-R3;CXCR-3;CKR-L2;G protein-coupled receptor 9;Interferon-inducible protein 10 receptor;IP-10 receptor;CD183;CXCR3;GPR9;
Research Areas |immunology|innate immunity|chemokines|alpha chemokine rec. (cxcr)| signal transduction|signaling pathway|calcium signaling|g protein signaling|gpcr| cardiovascular|atherosclerosis|vascular inflammation|leukocyte recruitment|
*if product is indicated to react with multiple species, protein info is based on the human gene.

Background for C-X-C chemokine receptor type 3

Chemokine receptor CXCR3 is a Galphai protein-coupled receptor in the CXC chemokine receptor family. Other names for CXCR3 are G protein-coupled receptor 9 (GPR9) and CD183. It is mapped to Xq13.1. CXCR3 is expressed on malignant B cells from chronic lymphoproliferative disorders, particularly in patients with CLL, and represents a fully functional receptor involved in chemotaxis of malignant B lymphocytes. It is found that in the absence of known etiologic agents, CXCR3 represents a novel target for therapeutic interference early in type 1 diabetes. CXCR3 signaling is associated with MG pathogenesis and proposed that and CXCR3 may serve as novel drug targets to treat MG. CXCR3A and CXCR3B are involved in the chemotactic and vascular effects of CXCL4L1.

Anti-CXCR3 Picoband™ Antibody Images

Click the images to enlarge.

Anti-CXCR3 Picoband™ Antibody
Anti-CXCR3 Picoband antibody, PB9079-1.JPG
IHC(P): Rat Kidney Tissue
Anti-CXCR3 Picoband™ Antibody
Anti-CXCR3 Picoband antibody, PB9079-2.JPG
IHC(P): Human Tonsil Tissue
Anti-CXCR3 Picoband™ Antibody
Anti-CXCR3 Picoband antibody, PB9079-3.JPG
IHC(P): Human Lung Cancer Tissue
Anti-CXCR3 Picoband™ Antibody
Anti-CXCR3 Picoband antibody, PB9079-4.jpg
All lanes: Anti-CXCR3(PB9079) at 0.5ug/ml
Lane 1: Colo320 Whole Cell Lysate at 40ug
Lane 2: SGC Whole Cell Lysate at 40ug
Predicted bind size: 41KD
Observed bind size: 41KD
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FAQs

Q: Do you offer BSA-free antibodies? Keyword: Bovine serum albumin, carrier protein, conjugation
A: Yes, please contact us at support@bosterbio.com for more information about BSA-free antibodies and availability. The new BSA-free formula uses trehalose as a replacement to BSA. We have tested many alternative chemicals and found that trehalose protects the antibodies the best.
Q: Can I conjugate markers to this antibody? Can I link custom conjugates to this antibody? Keyword: conjugation
A: The antibody is stored with BSA and cannot be conjugated with markers. Carrier free antibodies are available upon request. Please contact support@bosterbio.com
Q: What should I use for negative control?
A: Please contact us for negative control suggestions. You can also check expression databases such as genecards, uniprot etc. Due to logistic reasons, we do not sell serum or lysates that we use internally for positive or negative control.
Q: Where can I find troubleshooting information? What should I do if I have unexpected bands, high background, no signal, weak signal
A: You can find Boster's troubleshoot guides under tech support tab. Please contact us for further assistance on troubleshooting your experiment.
Q: What is the immunogen sequence of this antibody? Is this antibody polyclonal or monoclonal?
A: You can find the immunogen sequence under "Immunogen" and clonality in the product name.
Q: What is the expected band size? Why is it different than the observed band size?
A: The expected band size is predicted on the size of the protein. The actual band size may be affected by a few other factors including but not limited to:
1. Post-translational modification:phosphorylation, methylation, glycosylation etc. These modifications prevent SDS molecules from binding to the target protein and thus make the band size appear larger than expected
2. Post-translational cleavage: this can cause smaller bands and or multiple bands

3. Alternative splicing: the same gene can have alternative splicing patterns generating different size proteins, all with reactivities to the antibody.

4. Amino Acid R chain charge: SDS binds to positive charges. The different size and charge of the Amino Acid side chains can affect the amount of SDS binding and thus affect the observed band size.
5. Multimers: Multimers are usually broken up in reducing conditions. However if the interactions between the multimers are strong, the band may appear higher.,
Q: What is the suggested dilution ratio for Western Blot (WB), Immunohistochemistry (IHC) and or ELISA standards? What is the optimal pH for the sample?
A: Check the datasheet for the product for details on dilution ratios for different experiments. You can find the datasheet button on the right side of the product page.
Q: What is the protocol you used for your Western blotting (WB) and Immunohistochemistry (IHC)?
A: Check our protocols under the tech support tab.
Q: What are some alternative names that could be used to describe this product?
A: Some common names include but are not limited to cxcr3 antibody, ip10 antibody