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SKU:MA1032
Clonality:Monoclonal
Clone Number:CY-A1
Application:ICC, WB
Price: $200.00
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Overview

Product Name Anti-Cyclin A Antibody (Monoclonal, CY-A1)
SKU/Catalog Number MA1032
Description Mouse IgG monoclonal antibody for Cyclin A, cyclin A2 (CCNA2) detection. Tested with WB, ICC in Human;mouse;bovine. No cross reactivity with other proteins.
Cite This Product Anti-Cyclin A Antibody (Monoclonal, CY-A1) CY-A1 (Boster Biological Technology, Pleasanton CA, USA, Catalog # MA1032)
Replacement Item This antibody may replace the following items: sc-136253|sc-239|sc-239-X|sc-271645|sc-271645-X|sc-271682|sc-271682-X|sc-31084|sc-31085|sc-31086|sc-53227|sc-53228|sc-53229|sc-53230|sc-53231|sc-53232|sc-596|sc-596-G|sc-751|sc-751-X from Santa Cruz Biotechnology.
Host Mouse
Isotype Mouse IgG2a
Validated Species Bovine, Human, Mouse
Application ICC, WB

*Our Boster Guarantee covers the use of this product in the above tested applications.

**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.

Recommended Detection Systems Boster recommends Enhanced Chemiluminescent Kit with anti-Mouse IgG (EK1001) for Western blot, and HRP Conjugated anti-Mouse IgG Super Vision Assay Kit (SV0001-1) for ICC.
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2017!
Immunogen Recombinant bovine cyclin A.
Cross Reactivity No cross reactivity with other proteins
Pack Size 100μg/vial

Properties

Clonality Monoclonal
Clone Number CY-A1
Form Lyophilized
Contents Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.
*carrier free antibody available upon request.
Concentration Add 1ml of PBS buffer will yield a concentration of 100ug/ml.
Storage At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing.
Purification Ascites
Isotype Mouse IgG2a

Protein Target Info (Source: Uniprot.org)

You can check the tissue specificity below for information on selecting positive and negative control.

Gene Name Ccna2
Protein Name Cyclin-A2
Molecular Weight 47269 MW
Protein Function Essential for the control of the cell cycle at the G1/S (start) and the G2/M (mitosis) transitions. .
Tissue Specificity Ubiquitous. In the testis, expressed in germ cells and in the ovary, in both germline and somatic cells.
Sequence Similarities Belongs to the cyclin family. Cyclin AB subfamily.
Subcellular Localization Nucleus. Cytoplasm. Cytoplasmic when associated with SCAPER. .
Uniprot ID P51943
Alternative Names Cyclin-A2;Cyclin-A;Ccna2;Ccna, Cyca;
Research Areas |cell biology|cell cycle|cyclins| cell biology|cyclin a family| epigenetics and nuclear signaling| cancer| cardiovascular|lipids / lipoproteins|lipoproteins/apolipoproteins|
*if product is indicated to react with multiple species, protein info is based on the human gene.

Background for Cyclin-A2

The mammalian A-type cyclin family consists of 2 members, cyclin A1(CCNA1) and cyclin A2(CCNA2). Human cyclin A gene is assigned to 4q26-q27. Cyclin A protein is synthesized and localized into the nucleus at the onset of S phase in nontransformed mammalian fibroblasts.

Anti-Cyclin A Antibody (Monoclonal, CY-A1) Images

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Anti-Cyclin A Antibody (Monoclonal, CY-A1)
Anti-Cyclin A antibody (monoclonal), MA1032, Western blotting
Lane 1: HELA Cell Lysate
Lane 2: JURKAT Cell Lysate
Anti-Cyclin A Antibody (Monoclonal, CY-A1)
Anti-Cyclin A antibody (monoclonal), MA1032, ICC
ICC: A549 Cell
Anti-Cyclin A Antibody (Monoclonal, CY-A1)
Anti-Cyclin A antibody (monoclonal), MA1032, ICC
ICC: SMMC-7721 Cell
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Publications

Mao F, Wang B, Xiao Q, Xi G, Sun W, Zhang H, Ye F, Wan F, Guo D, Lei T, Chen X. Int J Oncol. 2013 Mar;42(3):1081-7. Doi: 10.3892/Ijo.2013.1776. Epub 2013 Jan 16. A Role For Lrig1 In The Regulation Of Malignant Glioma Aggressiveness.
Lin P, Fu J, Zhao B, Lin F, Zou H, Liu L, Zhu C, Wang H, Yu X. Mol Biol Rep. 2011 Mar;38(3):1741-7. Doi: 10.1007/S11033-010-0288-7. Epub 2010 Sep 29. Fbxw8 Is Involved In The Proliferation Of Human Choriocarcinoma Jeg-3 Cells.
Deng L, Lu Y, Zhao X, Sun Y, Shi Y, Fan H, Liu C, Zhou J, Nie Y, Wu K, Fan D, Guo X. Biochem Biophys Res Commun. 2013 Oct 18;440(2):322-9. Doi: 10.1016/J.Bbrc.2013.09.079. Epub 2013 Sep 25. Ran Gtpase Protein Promotes Human Pancreatic Cancer Proli...
Zhao P, Li Y, Gao G, Wang S, Yan Y, Zhan X, Liu Z, Mao Z, Chen S, Wang L. Eur J Med Chem. 2014 Oct 30;86:165-74. Doi: 10.1016/J.Ejmech.2014.08.049. Epub 2014 Aug 15. Design, Synthesis And Biological Evaluation Of N-Alkyl Or Aryl Substituted Isoind...
Li M, Tian L, Wang L, Yao H, Zhang J, Lu J, Sun Y, Gao X, Xiao H, Liu M. Plos One. 2013 Oct 23;8(10):E77829. Doi: 10.1371/Journal.Pone.0077829. Ecollection 2013. Down-Regulation Of Mir-129-5P Inhibits Growth And Induces Apoptosis In Laryngeal Squa...

FAQs

Q: Is your western blot protocol provided from the website applicable for all your antibodies? Keyword: applications, WB
A: The protocol is applicable for all our antibodies in WB, the NC Membrane(0.45μm or 0.22μm) and transfer time(70 mins or 50 mins) depends on the protein molecular weight, details can be found in included protocol.
Q: Can I conjugate markers to this antibody? Can I link custom conjugates to this antibody? Keyword: conjugation
A: The antibody is stored with BSA and cannot be conjugated with markers. Carrier free antibodies are available upon request. Please contact support@bosterbio.com
Q: Can you provide monoclonal antibodies carrier free?
A: We cannot.
Q: What are some alternative names that could be used to describe this product?
A: Some common names include but are not limited to cyclin-a antibody, ccn1 antibody, cyclin a2 antibody, cyclin-a2 antibody