|Application:||Flow Cytometry, IHC-F, ICC, WB|
Data & Images
|Product Name||Anti-Cyclin B1 Picoband™ Antibody|
|Description||Rabbit IgG polyclonal antibody for G2/mitotic-specific cyclin-B1(CCNB1) detection. Tested with WB, IHC-F, ICC, FCM in Human.|
|Cite This Product||Anti-Cyclin B1 Picoband™ Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9104)|
|Replacement Item||This antibody may replace the following items: sc-130591|sc-166757|sc-245|sc-245-X|sc-293098|sc-53236|sc-53238|sc-594|sc-595|sc-70898|sc-7393|sc-752 from Santa Cruz Biotechnology.|
|Application||Flow Cytometry, IHC-F, ICC, WB
*Our Boster Guarantee covers the use of this product in the above tested applications.
**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.
|Recommended Detection Systems||Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(F) and ICC.
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2017!
|Immunogen||E.coli-derived human Cyclin B1 recombinant protein (Position: M1-V433). Human Cyclin B1 shares 86% and 85% amino acid (aa) sequences identity with mouse and rat Cyclin B1, respectively.|
|Cross Reactivity||No cross reactivity with other proteins|
|Contents||Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
*carrier free antibody available upon request.
|Concentration||Add 0.2ml of distilled water will yield a concentration of 500ug/ml.|
|Storage||At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing.|
|Purification||Immunogen affinity purified.|
Protein Target Info (Source: Uniprot.org)
You can check the tissue specificity below for information on selecting positive and negative control.
|Protein Name||G2/mitotic-specific cyclin-B1|
|Molecular Weight||48337 MW|
|Protein Function||Essential for the control of the cell cycle at the G2/M (mitosis) transition. .|
|Sequence Similarities||Belongs to the cyclin family. Cyclin AB subfamily.|
|Subcellular Localization||Cytoplasm. Nucleus. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome.|
|Alternative Names||G2/mitotic-specific cyclin-B1;CCNB1;CCNB;|
|Research Areas|||cell biology|proteolysis / ubiquitin|proteasome / ubiquitin|ubiquitin e3 enzymes|ring finger e3 ligase| cell biology|cell cycle|cell division|other cell division antibodies||
Background for G2/mitotic-specific cyclin-B1
Dilution Ratios/Recommended Concentrations
At Boster we strive to provide the best Anti-Cyclin B1 Picoband™ Antibody by testing all applications on non-spiked tissues and cell lines to ensure that the affinity of the antibody is enough to react to the endogenouse level of the target protein. Read more about our QC panel here.
|Recommended dilution ratios are listed below:|
Western blot, 0.1-0.5μg/ml, Human|
Immunohistochemistry(Frozen Section), 0.5-1μg/ml, Human
Immunocytochemistry, 0.5-1μg/ml, Human
Flow Cytometry, 1-3μg/1x106 cells, Human
**Boster provides high sensitivity secondary antibody kits for Western blotting and IHC. For more info see Related Products below.
Anti-Cyclin B1 Picoband™ Antibody Images
Click the images to enlarge.
All lanes: Anti Cyclin B1 (PB9104) at 0.5ug/ml
WB: Recombinant Human Cyclin B1 Protein 0.5ng
Predicted bind size: 39KD
Observed bind size: 39KD
All lanes: Anti Cyclin B1 (PB9104) at 0.5ug/ml
Lane 1: HELA Whole Cell Lysate at 40ug
Lane 2: 293T Whole Cell Lysate at 40ug
Lane 3: MCF-7 Whole Cell Lysate at 40ug
Lane 4: COLO320 Whole Cell Lysate at 40ug
Predicted bind size: 48KD
Observed bind size: 56KD
1. Post-translational modification:phosphorylation, methylation, glycosylation etc. These modifications prevent SDS molecules from binding to the target protein and thus make the band size appear larger than expected
2. Post-translational cleavage: this can cause smaller bands and or multiple bands
3. Alternative splicing: the same gene can have alternative splicing patterns generating different size proteins, all with reactivities to the antibody.
4. Amino Acid R chain charge: SDS binds to positive charges. The different size and charge of the Amino Acid side chains can affect the amount of SDS binding and thus affect the observed band size.
5. Multimers: Multimers are usually broken up in reducing conditions. However if the interactions between the multimers are strong, the band may appear higher.,