|Validated Species:||Human, Mouse, Rat|
|Application:||IHC, ICC, WB|
Data & Images
|Product Name||Anti-Cyclin D1 Antibody (Monoclonal, DCS-6)|
|Description||Mouse IgG monoclonal antibody for Cyclin D1, cyclin D1 (CCND1) detection. Tested with WB, IHC-P, IHC-F, ICC in Human;mouse;rat. No cross reactivity with other proteins.|
|Cite This Product||Anti-Cyclin D1 Antibody (Monoclonal, DCS-6) DCS-6 (Boster Biological Technology, Pleasanton CA, USA, Catalog # MA1033)|
|Replacement Item||This antibody may replace the following items: sc-181|sc-181-G|sc-20044|sc-246|sc-376676|sc-450|sc-452|sc-56302|sc-70899|sc-717|sc-718|sc-753|sc-755|sc-8396 from Santa Cruz Biotechnology.|
|Validated Species||Human, Mouse, Rat|
|Application||IHC, ICC, WB
*Our Boster Guarantee covers the use of this product in the above tested applications.
**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.
|Recommended Detection Systems||Boster recommends Enhanced Chemiluminescent Kit with anti-Mouse IgG (EK1001) for Western blot, and HRP Conjugated anti-Mouse IgG Super Vision Assay Kit (SV0001-1) for IHC(P), IHC(F) and ICC.
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2018!
|Immunogen||Recombinant human cyclin D1 protein.|
|Cross Reactivity||No cross reactivity with other proteins|
|Contents||Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.|
|Concentration||Add 1ml of PBS buffer will yield a concentration of 100ug/ml.|
|Storage||At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing.|
Protein Target Info (Source: Uniprot.org)
You can check the tissue specificity below for information on selecting positive and negative control.
|Protein Name||G1/S-specific cyclin-D1|
|Molecular Weight||33483 MW|
|Protein Function||Regulatory component of the cyclin D1-CDK4 (DC) complex that phosphorylates and inhibits members of the retinoblastoma (RB) protein family including RB1 and regulates the cell-cycle during G(1)/S transition. Phosphorylation of RB1 allows dissociation of the transcription factor E2F from the RB/E2F complex and the subsequent transcription of E2F target genes which are responsible for the progression through the G(1) phase. Hypophosphorylates RB1 in early G(1) phase. Cyclin D-CDK4 complexes are major integrators of various mitogenenic and antimitogenic signals. Also substrate for SMAD3, phosphorylating SMAD3 in a cell-cycle-dependent manner and repressing its transcriptional activity. Component of the ternary complex, cyclin D1/CDK4/CDKN1B, required for nuclear translocation and activity of the cyclin D-CDK4 complex, Exhibits transcriptional corepressor activity with INSM1 on the NEUROD1 and INS promoters in a cell cycle-independent manner (By similarity). .|
|Sequence Similarities||Belongs to the cyclin family. Cyclin D subfamily.|
|Subcellular Localization||Nucleus . Cytoplasm . Membrane . Cyclin D-CDK4 complexes accumulate at the nuclear membrane and are then translocated into the nucleus through interaction with KIP/CIP family members. .|
|Alternative Names||G1/S-specific cyclin-D1;Ccnd1;|
Background for G1/S-specific cyclin-D1
Anti-Cyclin D1 Antibody (Monoclonal, DCS-6) Images
Click the images to enlarge.
IHC(F): Mouse Intestine Tissue
IHC(F): Mouse Spleen Tissue
IHC(F): Rat Intestine Tissue
IHC(F): Human Placenta Tissue