SKU MA1033
Size 100μg/vial
Reactivity Human, Mouse, Rat
Clonality Monoclonal
Clone Number DCS-6
Host Mouse
Ig Isotype Mouse IgG2a
Applications IHC, ICC, WB

Overview

Product Name Anti-Cyclin D1 Antibody (Monoclonal, DCS-6)
SKU/Catalog Number MA1033
Storage & Handling At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing.
Size 100μg/vial
Description Mouse IgG monoclonal antibody for Cyclin D1, cyclin D1 (CCND1) detection. Tested with WB, IHC-P, IHC-F, ICC in Human;mouse;rat. No cross reactivity with other proteins.
Cite This Product Anti-Cyclin D1 Antibody (Monoclonal, DCS-6) (Boster Biological Technology, Pleasanton CA, USA, Catalog # MA1033)
Host Mouse
Immunogen Recombinant human cyclin D1 protein.
Reactivity Human, Mouse, Rat

Assay Details

Assay Dilutions Overview

Immunohistochemistry(Paraffin-embedded Section), 8μg/ml, Human, mouse, rat, By Heat
Immunocytochemistry , 1μg/ml, Human, mouse, rat, -
Immunohistochemistry(Frozen Section), 8μg/ml, Human, mouse, rat, -
Western blot, 4μg/ml, Human, mouse, rat

Boster's Secondary Antibodies And IHC, WB Kits

The following reagents are used to generate the images below.

Boster recommends Enhanced Chemiluminescent Kit with anti-Mouse IgG (EK1001) for Western blot, and HRP Conjugated anti-Mouse IgG Super Vision Assay Kit (SV0001-1) for IHC(P), IHC(F) and ICC.

Images And Assay Conditions

/antibody/ma1033 1 IHC anti cyclin d1 antibody monoclonal dcs 6.jpg

Anti-Cyclin D1 antibody, MA1033, IHC(F)
IHC(F): Mouse Intestine Tissue

/antibody/ma1033 2 IHC anti cyclin d1 antibody monoclonal dcs 6.jpg

Anti-Cyclin D1 antibody, MA1033, IHC(F)
IHC(F): Mouse Spleen Tissue

/antibody/ma1033 3 IHC anti cyclin d1 antibody monoclonal dcs 6.jpg

Anti-Cyclin D1 antibody, MA1033, IHC(F)
IHC(F): Rat Intestine Tissue

/antibody/ma1033 4 IHC anti cyclin d1 antibody monoclonal dcs 6.jpg

Anti-Cyclin D1 antibody, MA1033, IHC(F)
IHC(F): Human Placenta Tissue

Target Info

Protein Target Info (Source: Uniprot.org)

Uniprot Id P39948
Gene Name Ccnd1
Protein Name G1/S-specific cyclin-D1
Alternative Names G1/S-specific cyclin-D1;Ccnd1;
Subcellular Localization Nucleus . Cytoplasm . Membrane . Cyclin D-CDK4 complexes accumulate at the nuclear membrane and are then translocated into the nucleus through interaction with KIP/CIP family members. .
Molecular Weight 33483 MW

*if product is indicated to react with multiple species, protein info is based on the human gene.

Ontology

Protein Function Regulatory component of the cyclin D1-CDK4 (DC) complex that phosphorylates and inhibits members of the retinoblastoma (RB) protein family including RB1 and regulates the cell-cycle during G(1)/S transition. Phosphorylation of RB1 allows dissociation of the transcription factor E2F from the RB/E2F complex and the subsequent transcription of E2F target genes which are responsible for the progression through the G(1) phase. Hypophosphorylates RB1 in early G(1) phase. Cyclin D-CDK4 complexes are major integrators of various mitogenenic and antimitogenic signals. Also substrate for SMAD3, phosphorylating SMAD3 in a cell-cycle-dependent manner and repressing its transcriptional activity. Component of the ternary complex, cyclin D1/CDK4/CDKN1B, required for nuclear translocation and activity of the cyclin D-CDK4 complex, Exhibits transcriptional corepressor activity with INSM1 on the NEUROD1 and INS promoters in a cell cycle-independent manner (By similarity). .
Background D-type cyclins(cyclins D1, D2, and D3) are regarded as essential links between cell environment and the core cell cycle machinery. CyclinD1(CCND1), also known as BCL1. Expression of cyclin D1, but not of cyclins A and E, was induced by transfection of the RB gene into RB-deficient tumor cells. CCND1 gene to 11q13. cyclin D1 gene can function as an oncogene. Cyclin D1 expression is regulated by the retinoblastoma protein.

Order Product (MA1033)

Promotion:

Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
Option Price
30ug sample size $99
100ug $280
100ug+Free HRP Secondary BA1050 $280
100ug+Free Biotin Secondary BA1001 $280

USD $280

Ships in 5-7 business days.

Troubleshooting

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Publications

Triptolide inhibits proliferation, differentiation and induces apoptosis of osteoblastic MC3T3-E1 cells
LRIG2 promotes the proliferation and cell cycle progression of glioblastoma cells in vitro and in vivo through enhancing PDGFR? signaling
Arginine deiminase augments the chemosensitivity of argininosuccinate synthetase-deficient pancreatic cancer cells to gemcitabine via inhibition of NF-?B signaling
Up-Regulation of Long Noncoding RNA SRA Promotes Cell Growth, Inhibits Cell Apoptosis, and Induces Secretion of Estradiol and Progesterone in Ovarian Granular Cells of Mice
Effects of low-intensity ultrasound combined with low-dose carboplatin in an orthotopic hamster model of tongue cancer: A preclinical study
Piwil2 is reactivated by HPV oncoproteins and initiates cell reprogramming via epigenetic regulation during cervical cancer tumorigenesis
Silencing of carboxypeptidase E inhibits cell proliferation, tumorigenicity, and metastasis of osteosarcoma cells
Fucoidan inhibits lymphangiogenesis by downregulating the expression of VEGFR3 and PROX1 in human lymphatic endothelial cells
TALENs-directedknockoutof thefull-lengthtranscriptionfactorNrf1? thatrepressesmalignantbehaviourofhumanhepatocellularcarcinoma(HepG2)cells
Sirt1 AS lncRNA interacts with its mRNA to inhibit muscle formation by attenuating function of miR-34a
The expression of AEG-1 and Cyclin D1 in human bladder urothelial carcinoma and their clinicopathological significance
Overexpression of miR-30a in lung adenocarcinoma A549 cell line inhibits migration and invasion via targeting EYA2
CD11b deficiency suppresses intestinal tumor growth by reducing myeloid cell recruitment
Glycyrrhizic acid alleviates bleomycin-induced pulmonary fibrosis in rats
miR-204-5p promotes the adipogenic differentiation of human adipose-derived mesenchymal stem cells by modulating DVL3 expression and suppressing Wnt/?-catenin signaling
Reduced expression of SOX7 in ovarian cancer: a novel tumor suppressor through the Wnt/?-catenin signaling pathway
Androglobin knockdown inhibits growth of glioma cell lines
Estrogen Induces Vav1 Expression in Human Breast Cancer Cells
Melittin Restores PTEN Expression by Down-Regulating HDAC2 in Human Hepatocelluar Carcinoma HepG2 Cells
ZFX knockdown inhibits growth and migration of non-small cell lung carcinoma cell line H1299
RhoC Regulates the Proliferation of Gastric Cancer Cells through Interaction with IQGAP1
He H, Chen K, Wang F, Zhao L, Wan X, Wang L, Mo Z. Int J Mol Med. 2015 Jun;35(6):1587-95. Doi: 10.3892/Ijmm.2015.2160. Epub 2015 Mar 31. Mir-204-5P Promotes The Adipogenic Differentiation Of Human Adipose-Derived Mesenchymal Stem Cells By Modulati...
Li L, Zhao F, Lu J, Li T, Yang H, Wu C, Liu Y. Plos One. 2014 Apr 23;9(4):E95912. Doi: 10.1371/Journal.Pone.0095912. Ecollection 2014. Notch-1 Signaling Promotes The Malignant Features Of Human Breast Cancer Through Nf-??b Activation.
Liu C, Su T, Li F, Li L, Qin X, Pan W, Feng F, Chen F, Liao D, Chen L. Acta Biochim Biophys Sin (Shanghai). 2010 Jun 15;42(6):396-402. Pi3K/Akt Signaling Transduction Pathway Is Involved In Rat Vascular Smooth Muscle Cell Proliferation Induced By ...
Feng Z, Chen J, Wei H, Gao P, Shi J, Zhang J, Zhao F. Exp Mol Pathol. 2011 Oct;91(2):569-77. Doi: 10.1016/J.Yexmp.2011.06.004. Epub 2011 Jul 12. The Risk Factor Of Gallbladder Cancer: Hyperplasia Of Mucous Epithelium Caused By Gallstones Associate...
Li B, Gao Mh, Chu Xm, Teng L, Lv Cy, Yang P, Yin Qf. Eur J Pharmacol. 2015 Feb 15;749:107-14. Doi: 10.1016/J.Ejphar.2015.01.009. Epub 2015 Jan 21. The Synergistic Antitumor Effects Of All-Trans Retinoic Acid And C-Phycocyanin On The Lung Cancer A5...
Nie D, Huang K, Yin S, Li Y, Xie S, Ma L, Wang X, Wu Y, Xiao J. Leuk Lymphoma. 2012 Dec;53(12):2487-95. Doi: 10.3109/10428194.2012.698273. Epub 2012 Jun 18. Synergistic/Additive Interaction Of Valproic Acid With Bortezomib On Proliferation And Apo...
Wu Y, Tao Y, Chen Y, Xu W. Plos One. 2012;7(11):E48917. Doi: 10.1371/Journal.Pone.0048917. Epub 2012 Nov 7. Rhoc Regulates The Proliferation Of Gastric Cancer Cells Through Interaction With Iqgap1.
Cai H, Wang Z, Zhang Hq, Wang Fr, Yu Cx, Zhang Fx, Gao L, Zhang J, Zhao Jj. Acta Pharmacol Sin. 2014 Jan;35(1):65-73. Doi: 10.1038/Aps.2013.133. Epub 2013 Nov 18. Diosgenin Relieves Goiter Via The Inhibition Of Thyrocyte Proliferation In A Mouse M...
Yang L, Su T, Lv D, Xie F, Liu W, Cao J, Sheikh Ia, Qin X, Li L, Chen L. Acta Biochim Biophys Sin (Shanghai). 2014 Feb;46(2):100-11. Doi: 10.1093/Abbs/Gmt140. Epub 2013 Dec 29. Erk1/2 Mediates Lung Adenocarcinoma Cell Proliferation And Autophagy I...
Du Mj, Chen Xd, Zhou Xl, Wan Yj, Lan B, Zhang Cz, Cao Y. Plos One. 2014 Jun 6;9(6):E99052. Doi: 10.1371/Journal.Pone.0099052. Ecollection 2014. Estrogen Induces Vav1 Expression In Human Breast Cancer Cells.
Huang B, Lu Ys, Li X, Zhu Zc, Li K, Liu Jw, Zheng J, Hu Zl. Int J Clin Exp Pathol. 2014 Apr 15;7(5):2179-84. Ecollection 2014. Androglobin Knockdown Inhibits Growth Of Glioma Cell Lines.
Liu H, Yan Zq, Li B, Yin Sy, Sun Q, Kou Jj, Ye D, Ferns K, Liu Hy, Liu Sl. J Ovarian Res. 2014 Sep 5;7:87. Doi: 10.1186/S13048-014-0087-1. Reduced Expression Of Sox7 In Ovarian Cancer: A Novel Tumor Suppressor Through The Wnt/??-Catenin Signaling P...
Liu J, Ma J, Wu Z, Li W, Zhang D, Han L, Wang F, Reindl Km, Wu E, Ma Q. Bmc Cancer. 2014 Sep 20;14:686. Doi: 10.1186/1471-2407-14-686. Arginine Deiminase Augments The Chemosensitivity Of Argininosuccinate Synthetase-Deficient Pancreatic Cancer Cel...
Zhu Z, Li K, Xu D, Liu Y, Tang H, Xie Q, Xie L, Liu J, Wang H, Gong Y, Hu Z, Zheng J. J Neurooncol. 2013 Mar;112(1):17-25. Doi: 10.1007/S11060-012-1032-Z. Epub 2013 Jan 16. Zfx Regulates Glioma Cell Proliferation And Survival In Vitro And In Vivo.
Liu Y, Zhu Z, Xiong Z, Zheng J, Hu Z, Qiu J. Int J Clin Exp Pathol. 2014 Aug 15;7(9):5750-61. Ecollection 2014. Knockdown Of Protein Tyrosine Phosphatase Receptor U Inhibits Growth And Motility Of Gastric Cancer Cells.
Zhu Z, Liu Y, Li K, Liu J, Wang H, Sun B, Xiong Z, Jiang H, Zheng J, Hu Z. Carcinogenesis. 2014 Aug;35(8):1901-10. Doi: 10.1093/Carcin/Bgu123. Epub 2014 May 29. Protein Tyrosine Phosphatase Receptor U (Ptpru) Is Required For Glioma Growth And Moti...
Deng L, Lu Y, Zhao X, Sun Y, Shi Y, Fan H, Liu C, Zhou J, Nie Y, Wu K, Fan D, Guo X. Biochem Biophys Res Commun. 2013 Oct 18;440(2):322-9. Doi: 10.1016/J.Bbrc.2013.09.079. Epub 2013 Sep 25. Ran Gtpase Protein Promotes Human Pancreatic Cancer Proli...
Li M, Tian L, Wang L, Yao H, Zhang J, Lu J, Sun Y, Gao X, Xiao H, Liu M. Plos One. 2013 Oct 23;8(10):E77829. Doi: 10.1371/Journal.Pone.0077829. Ecollection 2013. Down-Regulation Of Mir-129-5P Inhibits Growth And Induces Apoptosis In Laryngeal Squa...
Zhang H, Zhao B, Huang C, Meng Xm, Bian Eb, Li J. Plos One. 2014 May 2;9(5):E95520. Doi: 10.1371/Journal.Pone.0095520. Ecollection 2014. Melittin Restores Pten Expression By Down-Regulating Hdac2 In Human Hepatocelluar Carcinoma Hepg2 Cells.
Han W, Liu Gn. Life Sci. 2010 Feb 13;86(7-8):234-43. Doi: 10.1016/J.Lfs.2009.12.005. Epub 2009 Dec 16. Egr-1 Decoy Odns Inhibit Vascular Smooth Muscle Cell Proliferation And Neointimal Hyperplasia Of Balloon-Injured Arteries In Rat.
Ren J, Huang Q, Xu Y, Yang M, Yang J, Hu K. Anticancer Drugs. 2015 Jul;26(6):599-611. Doi: 10.1097/Cad.0000000000000224. Isoflavone Lupiwighteone Induces Cytotoxic, Apoptotic, And Antiangiogenic Activities In Du-145 Prostate Cancer Cells.
Li K, Zhu Zc, Liu Yj, Liu Jw, Wang Ht, Xiong Zq, Shen X, Hu Zl, Zheng J. Int J Clin Exp Pathol. 2013 Sep 15;6(11):2460-7. Ecollection 2013. Zfx Knockdown Inhibits Growth And Migration Of Non-Small Cell Lung Carcinoma Cell Line H1299.
Li B, Xu Yj, Chu Xm, Gao Mh, Wang Xh, Nie Sm, Yang F, Lv Cy. Immunol Lett. 2013 Nov-Dec;156(1-2):68-81. Doi: 10.1016/J.Imlet.2013.09.025. Epub 2013 Sep 29. Molecular Mechanism Of Inhibitory Effects Of Cd59 Gene On Atherosclerosis In Apoe (-/-) Mice.

Customer Q&As

Q: Is your western blot protocol provided from the website applicable for all your antibodies? Keyword: applications, WB
A: The protocol is applicable for all our antibodies in WB, the NC Membrane(0.45μm or 0.22μm) and transfer time(70 mins or 50 mins) depends on the protein molecular weight, details can be found in included protocol.
Q: Can I conjugate markers to this antibody? Can I link custom conjugates to this antibody? Keyword: conjugation
A: The antibody is stored with BSA and cannot be conjugated with markers. Carrier free antibodies are available upon request. Please contact support@bosterbio.com
Q: Can you provide monoclonal antibodies carrier free?
A: We cannot.
Q: What are some alternative names that could be used to describe this product?
A: Some common names include but are not limited to cyclin d1 antibody, cd1 antibody, ccnd1 antibody