|Application:||IHC-P, IHC-F, ICC, WB|
Data & Images
|Product Name||Anti-Cyclin D1 Antibody (Monoclonal, DCS-6)|
|Description||Mouse IgG monoclonal antibody for Cyclin D1, cyclin D1 (CCND1) detection. Tested with WB, IHC-P, IHC-F, ICC in Human;mouse;rat. No cross reactivity with other proteins.|
|Cite This Product||Anti-Cyclin D1 Antibody (Monoclonal, DCS-6) DCS-6 (Boster Biological Technology, Pleasanton CA, USA, Catalog # MA1033)|
|Replacement Item||This antibody may replace the following items: sc-181|sc-181-G|sc-20044|sc-246|sc-376676|sc-450|sc-452|sc-56302|sc-70899|sc-717|sc-718|sc-753|sc-755|sc-8396 from Santa Cruz Biotechnology.|
|Validated Species||Human, Mouse, Rat|
|Application||IHC-P, IHC-F, ICC, WB
*Our Boster Guarantee covers the use of this product in the above tested applications.
**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.
|Recommended Detection Systems||Boster recommends Enhanced Chemiluminescent Kit with anti-Mouse IgG (EK1001) for Western blot, and HRP Conjugated anti-Mouse IgG Super Vision Assay Kit (SV0001-1) for IHC(P), IHC(F) and ICC.
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2017!
|Immunogen||Recombinant human cyclin D1 protein.|
|Cross Reactivity||No cross reactivity with other proteins|
|Contents||Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.
*carrier free antibody available upon request.
|Concentration||Add 1ml of PBS buffer will yield a concentration of 100ug/ml.|
|Storage||At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing.|
Protein Target Info (Source: Uniprot.org)
You can check the tissue specificity below for information on selecting positive and negative control.
|Protein Name||G1/S-specific cyclin-D1|
|Molecular Weight||33483 MW|
|Protein Function||Regulatory component of the cyclin D1-CDK4 (DC) complex that phosphorylates and inhibits members of the retinoblastoma (RB) protein family including RB1 and regulates the cell-cycle during G(1)/S transition. Phosphorylation of RB1 allows dissociation of the transcription factor E2F from the RB/E2F complex and the subsequent transcription of E2F target genes which are responsible for the progression through the G(1) phase. Hypophosphorylates RB1 in early G(1) phase. Cyclin D-CDK4 complexes are major integrators of various mitogenenic and antimitogenic signals. Also substrate for SMAD3, phosphorylating SMAD3 in a cell-cycle-dependent manner and repressing its transcriptional activity. Component of the ternary complex, cyclin D1/CDK4/CDKN1B, required for nuclear translocation and activity of the cyclin D-CDK4 complex, Exhibits transcriptional corepressor activity with INSM1 on the NEUROD1 and INS promoters in a cell cycle-independent manner (By similarity). .|
|Sequence Similarities||Belongs to the cyclin family. Cyclin D subfamily.|
|Subcellular Localization||Nucleus . Cytoplasm . Membrane . Cyclin D-CDK4 complexes accumulate at the nuclear membrane and are then translocated into the nucleus through interaction with KIP/CIP family members. .|
|Alternative Names||G1/S-specific cyclin-D1;Ccnd1;|
Background for G1/S-specific cyclin-D1
Dilution Ratios/Recommended Concentrations
At Boster we strive to provide the best Anti-Cyclin D1 Antibody (Monoclonal, DCS-6) by testing all applications on non-spiked tissues and cell lines to ensure that the affinity of the antibody is enough to react to the endogenouse level of the target protein. Read more about our QC panel here.
|Recommended dilution ratios are listed below:|
Immunohistochemistry(Paraffin-embedded Section), 8μg/ml, Human, mouse, rat, By Heat
Immunocytochemistry , 1μg/ml, Human, mouse, rat, -
Immunohistochemistry(Frozen Section), 8μg/ml, Human, mouse, rat, -
Western blot, 4μg/ml, Human, mouse, rat
**Boster provides high sensitivity secondary antibody kits for Western blotting and IHC. For more info see Related Products below.
Anti-Cyclin D1 Antibody (Monoclonal, DCS-6) Images
Click the images to enlarge.
IHC(F): Mouse Intestine Tissue
IHC(F): Mouse Spleen Tissue
IHC(F): Rat Intestine Tissue
IHC(F): Human Placenta Tissue