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SKU:MA1033
Pack Size:100μg/vial
Sample Size:30ug for $99, contact us for details
Clonality:Monoclonal
Clone Number:DCS-6
Application:IHC-P, IHC-F, ICC, WB
Price: $200.00
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Overview

Product Name Anti-Cyclin D1 Antibody (Monoclonal, DCS-6)
SKU/Catalog Number MA1033
Description Mouse IgG monoclonal antibody for Cyclin D1, cyclin D1 (CCND1) detection. Tested with WB, IHC-P, IHC-F, ICC in Human;mouse;rat. No cross reactivity with other proteins.
Cite This Product Anti-Cyclin D1 Antibody (Monoclonal, DCS-6) DCS-6 (Boster Biological Technology, Pleasanton CA, USA, Catalog # MA1033)
Replacement Item This antibody may replace the following items: sc-181|sc-181-G|sc-20044|sc-246|sc-376676|sc-450|sc-452|sc-56302|sc-70899|sc-717|sc-718|sc-753|sc-755|sc-8396 from Santa Cruz Biotechnology.
Host Mouse
Isotype Mouse IgG2a
Validated Species Human, Mouse, Rat
Application IHC-P, IHC-F, ICC, WB

*Our Boster Guarantee covers the use of this product in the above tested applications.

**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.

Recommended Detection Systems Boster recommends Enhanced Chemiluminescent Kit with anti-Mouse IgG (EK1001) for Western blot, and HRP Conjugated anti-Mouse IgG Super Vision Assay Kit (SV0001-1) for IHC(P), IHC(F) and ICC.
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2017!
Immunogen Recombinant human cyclin D1 protein.
Cross Reactivity No cross reactivity with other proteins
Pack Size 100μg/vial

Properties

Clonality Monoclonal
Clone Number DCS-6
Form Lyophilized
Contents Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.
*carrier free antibody available upon request.
Concentration Add 1ml of PBS buffer will yield a concentration of 100ug/ml.
Storage At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing.
Purification Ascites
Isotype Mouse IgG2a

Protein Target Info (Source: Uniprot.org)

You can check the tissue specificity below for information on selecting positive and negative control.

Gene Name Ccnd1
Protein Name G1/S-specific cyclin-D1
Molecular Weight 33483 MW
Protein Function Regulatory component of the cyclin D1-CDK4 (DC) complex that phosphorylates and inhibits members of the retinoblastoma (RB) protein family including RB1 and regulates the cell-cycle during G(1)/S transition. Phosphorylation of RB1 allows dissociation of the transcription factor E2F from the RB/E2F complex and the subsequent transcription of E2F target genes which are responsible for the progression through the G(1) phase. Hypophosphorylates RB1 in early G(1) phase. Cyclin D-CDK4 complexes are major integrators of various mitogenenic and antimitogenic signals. Also substrate for SMAD3, phosphorylating SMAD3 in a cell-cycle-dependent manner and repressing its transcriptional activity. Component of the ternary complex, cyclin D1/CDK4/CDKN1B, required for nuclear translocation and activity of the cyclin D-CDK4 complex, Exhibits transcriptional corepressor activity with INSM1 on the NEUROD1 and INS promoters in a cell cycle-independent manner (By similarity). .
Sequence Similarities Belongs to the cyclin family. Cyclin D subfamily.
Subcellular Localization Nucleus . Cytoplasm . Membrane . Cyclin D-CDK4 complexes accumulate at the nuclear membrane and are then translocated into the nucleus through interaction with KIP/CIP family members. .
Uniprot ID P39948
Alternative Names G1/S-specific cyclin-D1;Ccnd1;
Research Areas CCND1|
*if product is indicated to react with multiple species, protein info is based on the human gene.

Background for G1/S-specific cyclin-D1

D-type cyclins(cyclins D1, D2, and D3) are regarded as essential links between cell environment and the core cell cycle machinery. CyclinD1(CCND1), also known as BCL1. Expression of cyclin D1, but not of cyclins A and E, was induced by transfection of the RB gene into RB-deficient tumor cells. CCND1 gene to 11q13. cyclin D1 gene can function as an oncogene. Cyclin D1 expression is regulated by the retinoblastoma protein.

Anti-Cyclin D1 Antibody (Monoclonal, DCS-6) Images

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Anti-Cyclin D1 Antibody (Monoclonal, DCS-6)
Anti-Cyclin D1 antibody, MA1033, IHC(F)
IHC(F): Mouse Intestine Tissue
Anti-Cyclin D1 Antibody (Monoclonal, DCS-6)
Anti-Cyclin D1 antibody, MA1033, IHC(F)
IHC(F): Mouse Spleen Tissue
Anti-Cyclin D1 Antibody (Monoclonal, DCS-6)
Anti-Cyclin D1 antibody, MA1033, IHC(F)
IHC(F): Rat Intestine Tissue
Anti-Cyclin D1 Antibody (Monoclonal, DCS-6)
Anti-Cyclin D1 antibody, MA1033, IHC(F)
IHC(F): Human Placenta Tissue
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Publications

He H, Chen K, Wang F, Zhao L, Wan X, Wang L, Mo Z. Int J Mol Med. 2015 Jun;35(6):1587-95. Doi: 10.3892/Ijmm.2015.2160. Epub 2015 Mar 31. Mir-204-5P Promotes The Adipogenic Differentiation Of Human Adipose-Derived Mesenchymal Stem Cells By Modulati...
Li L, Zhao F, Lu J, Li T, Yang H, Wu C, Liu Y. Plos One. 2014 Apr 23;9(4):E95912. Doi: 10.1371/Journal.Pone.0095912. Ecollection 2014. Notch-1 Signaling Promotes The Malignant Features Of Human Breast Cancer Through Nf-??b Activation.
Liu C, Su T, Li F, Li L, Qin X, Pan W, Feng F, Chen F, Liao D, Chen L. Acta Biochim Biophys Sin (Shanghai). 2010 Jun 15;42(6):396-402. Pi3K/Akt Signaling Transduction Pathway Is Involved In Rat Vascular Smooth Muscle Cell Proliferation Induced By ...
Feng Z, Chen J, Wei H, Gao P, Shi J, Zhang J, Zhao F. Exp Mol Pathol. 2011 Oct;91(2):569-77. Doi: 10.1016/J.Yexmp.2011.06.004. Epub 2011 Jul 12. The Risk Factor Of Gallbladder Cancer: Hyperplasia Of Mucous Epithelium Caused By Gallstones Associate...
Li B, Gao Mh, Chu Xm, Teng L, Lv Cy, Yang P, Yin Qf. Eur J Pharmacol. 2015 Feb 15;749:107-14. Doi: 10.1016/J.Ejphar.2015.01.009. Epub 2015 Jan 21. The Synergistic Antitumor Effects Of All-Trans Retinoic Acid And C-Phycocyanin On The Lung Cancer A5...
Nie D, Huang K, Yin S, Li Y, Xie S, Ma L, Wang X, Wu Y, Xiao J. Leuk Lymphoma. 2012 Dec;53(12):2487-95. Doi: 10.3109/10428194.2012.698273. Epub 2012 Jun 18. Synergistic/Additive Interaction Of Valproic Acid With Bortezomib On Proliferation And Apo...
Wu Y, Tao Y, Chen Y, Xu W. Plos One. 2012;7(11):E48917. Doi: 10.1371/Journal.Pone.0048917. Epub 2012 Nov 7. Rhoc Regulates The Proliferation Of Gastric Cancer Cells Through Interaction With Iqgap1.
Cai H, Wang Z, Zhang Hq, Wang Fr, Yu Cx, Zhang Fx, Gao L, Zhang J, Zhao Jj. Acta Pharmacol Sin. 2014 Jan;35(1):65-73. Doi: 10.1038/Aps.2013.133. Epub 2013 Nov 18. Diosgenin Relieves Goiter Via The Inhibition Of Thyrocyte Proliferation In A Mouse M...
Yang L, Su T, Lv D, Xie F, Liu W, Cao J, Sheikh Ia, Qin X, Li L, Chen L. Acta Biochim Biophys Sin (Shanghai). 2014 Feb;46(2):100-11. Doi: 10.1093/Abbs/Gmt140. Epub 2013 Dec 29. Erk1/2 Mediates Lung Adenocarcinoma Cell Proliferation And Autophagy I...
Du Mj, Chen Xd, Zhou Xl, Wan Yj, Lan B, Zhang Cz, Cao Y. Plos One. 2014 Jun 6;9(6):E99052. Doi: 10.1371/Journal.Pone.0099052. Ecollection 2014. Estrogen Induces Vav1 Expression In Human Breast Cancer Cells.
Huang B, Lu Ys, Li X, Zhu Zc, Li K, Liu Jw, Zheng J, Hu Zl. Int J Clin Exp Pathol. 2014 Apr 15;7(5):2179-84. Ecollection 2014. Androglobin Knockdown Inhibits Growth Of Glioma Cell Lines.
Liu H, Yan Zq, Li B, Yin Sy, Sun Q, Kou Jj, Ye D, Ferns K, Liu Hy, Liu Sl. J Ovarian Res. 2014 Sep 5;7:87. Doi: 10.1186/S13048-014-0087-1. Reduced Expression Of Sox7 In Ovarian Cancer: A Novel Tumor Suppressor Through The Wnt/??-Catenin Signaling P...
Liu J, Ma J, Wu Z, Li W, Zhang D, Han L, Wang F, Reindl Km, Wu E, Ma Q. Bmc Cancer. 2014 Sep 20;14:686. Doi: 10.1186/1471-2407-14-686. Arginine Deiminase Augments The Chemosensitivity Of Argininosuccinate Synthetase-Deficient Pancreatic Cancer Cel...
Zhu Z, Li K, Xu D, Liu Y, Tang H, Xie Q, Xie L, Liu J, Wang H, Gong Y, Hu Z, Zheng J. J Neurooncol. 2013 Mar;112(1):17-25. Doi: 10.1007/S11060-012-1032-Z. Epub 2013 Jan 16. Zfx Regulates Glioma Cell Proliferation And Survival In Vitro And In Vivo.
Liu Y, Zhu Z, Xiong Z, Zheng J, Hu Z, Qiu J. Int J Clin Exp Pathol. 2014 Aug 15;7(9):5750-61. Ecollection 2014. Knockdown Of Protein Tyrosine Phosphatase Receptor U Inhibits Growth And Motility Of Gastric Cancer Cells.
Zhu Z, Liu Y, Li K, Liu J, Wang H, Sun B, Xiong Z, Jiang H, Zheng J, Hu Z. Carcinogenesis. 2014 Aug;35(8):1901-10. Doi: 10.1093/Carcin/Bgu123. Epub 2014 May 29. Protein Tyrosine Phosphatase Receptor U (Ptpru) Is Required For Glioma Growth And Moti...
Deng L, Lu Y, Zhao X, Sun Y, Shi Y, Fan H, Liu C, Zhou J, Nie Y, Wu K, Fan D, Guo X. Biochem Biophys Res Commun. 2013 Oct 18;440(2):322-9. Doi: 10.1016/J.Bbrc.2013.09.079. Epub 2013 Sep 25. Ran Gtpase Protein Promotes Human Pancreatic Cancer Proli...
Li M, Tian L, Wang L, Yao H, Zhang J, Lu J, Sun Y, Gao X, Xiao H, Liu M. Plos One. 2013 Oct 23;8(10):E77829. Doi: 10.1371/Journal.Pone.0077829. Ecollection 2013. Down-Regulation Of Mir-129-5P Inhibits Growth And Induces Apoptosis In Laryngeal Squa...
Zhang H, Zhao B, Huang C, Meng Xm, Bian Eb, Li J. Plos One. 2014 May 2;9(5):E95520. Doi: 10.1371/Journal.Pone.0095520. Ecollection 2014. Melittin Restores Pten Expression By Down-Regulating Hdac2 In Human Hepatocelluar Carcinoma Hepg2 Cells.
Han W, Liu Gn. Life Sci. 2010 Feb 13;86(7-8):234-43. Doi: 10.1016/J.Lfs.2009.12.005. Epub 2009 Dec 16. Egr-1 Decoy Odns Inhibit Vascular Smooth Muscle Cell Proliferation And Neointimal Hyperplasia Of Balloon-Injured Arteries In Rat.
Ren J, Huang Q, Xu Y, Yang M, Yang J, Hu K. Anticancer Drugs. 2015 Jul;26(6):599-611. Doi: 10.1097/Cad.0000000000000224. Isoflavone Lupiwighteone Induces Cytotoxic, Apoptotic, And Antiangiogenic Activities In Du-145 Prostate Cancer Cells.
Li K, Zhu Zc, Liu Yj, Liu Jw, Wang Ht, Xiong Zq, Shen X, Hu Zl, Zheng J. Int J Clin Exp Pathol. 2013 Sep 15;6(11):2460-7. Ecollection 2013. Zfx Knockdown Inhibits Growth And Migration Of Non-Small Cell Lung Carcinoma Cell Line H1299.
Li B, Xu Yj, Chu Xm, Gao Mh, Wang Xh, Nie Sm, Yang F, Lv Cy. Immunol Lett. 2013 Nov-Dec;156(1-2):68-81. Doi: 10.1016/J.Imlet.2013.09.025. Epub 2013 Sep 29. Molecular Mechanism Of Inhibitory Effects Of Cd59 Gene On Atherosclerosis In Apoe (-/-) Mice.

FAQs

Q: Is your western blot protocol provided from the website applicable for all your antibodies? Keyword: applications, WB
A: The protocol is applicable for all our antibodies in WB, the NC Membrane(0.45μm or 0.22μm) and transfer time(70 mins or 50 mins) depends on the protein molecular weight, details can be found in included protocol.
Q: Can I conjugate markers to this antibody? Can I link custom conjugates to this antibody? Keyword: conjugation
A: The antibody is stored with BSA and cannot be conjugated with markers. Carrier free antibodies are available upon request. Please contact support@bosterbio.com
Q: Can you provide monoclonal antibodies carrier free?
A: We cannot.
Q: What are some alternative names that could be used to describe this product?
A: Some common names include but are not limited to cyclin d1 antibody, cd1 antibody, ccnd1 antibody